##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512294_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1701267 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.446463723801145 31.0 30.0 34.0 15.0 34.0 2 29.365200171401668 31.0 30.0 34.0 16.0 34.0 3 30.02260785637998 31.0 30.0 34.0 25.0 34.0 4 33.65458449496757 37.0 35.0 37.0 28.0 37.0 5 33.82322939315228 37.0 35.0 37.0 30.0 37.0 6 33.976750269064176 37.0 35.0 37.0 30.0 37.0 7 33.913155900866826 37.0 35.0 37.0 30.0 37.0 8 33.902948214477796 37.0 35.0 37.0 30.0 37.0 9 35.41853806604137 39.0 37.0 39.0 30.0 39.0 10 35.31703077764983 39.0 37.0 39.0 30.0 39.0 11 35.34725589810418 39.0 37.0 39.0 30.0 39.0 12 35.27060596602414 39.0 37.0 39.0 30.0 39.0 13 35.248362543915796 39.0 37.0 39.0 30.0 39.0 14 36.3969429842582 40.0 37.0 41.0 29.0 41.0 15 36.335822654527476 40.0 37.0 41.0 29.0 41.0 16 36.257446949832094 40.0 37.0 41.0 28.0 41.0 17 36.20975602301109 40.0 37.0 41.0 28.0 41.0 18 35.997706415277555 40.0 37.0 41.0 27.0 41.0 19 36.089996455582806 40.0 37.0 41.0 27.0 41.0 20 36.023593592305026 40.0 37.0 41.0 27.0 41.0 21 35.9477124989787 40.0 37.0 41.0 27.0 41.0 22 35.862239730741855 40.0 37.0 41.0 27.0 41.0 23 35.72869749427927 40.0 37.0 41.0 27.0 41.0 24 35.59877550084731 40.0 36.0 41.0 27.0 41.0 25 35.47410136092689 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6506714970322207 1101 2 -0.09527418165813373 1101 3 -0.4902530875479094 1101 4 -0.6744649375040837 1101 5 -0.8816198239860213 1101 6 -1.0123263085307102 1101 7 -1.1158883226271712 1101 8 -1.0314283098188781 1101 9 -1.1656314042094849 1101 10 -1.2408425877006266 1101 11 -1.3708225065728286 1101 12 -1.4957634778499127 1101 13 -1.539416178485908 1101 14 -1.8170108486205763 1101 15 -1.8645440850365702 1101 16 -1.8553768766936187 1101 17 -2.0090915077652127 1101 18 -2.020394140759656 1101 19 -1.804929802517556 1101 20 -2.014786495580509 1101 21 -2.036286376364309 1101 22 -2.0027002905448654 1101 23 -2.4778358272165377 1101 24 -2.7567605194089495 1101 25 -2.6800554730044297 1102 1 0.9680972767515321 1102 2 0.2624114952246295 1102 3 -0.3014393641383286 1102 4 -0.23524763349403344 1102 5 -0.4214250720042827 1102 6 -0.5455272213263029 1102 7 -0.4714330948376144 1102 8 -0.7206915159060898 1102 9 -0.7715300794477216 1102 10 -0.8413279971988246 1102 11 -1.0170271797619606 1102 12 -1.218259390379231 1102 13 -1.0146791318136295 1102 14 -1.1627691390126174 1102 15 -1.10030311463089 1102 16 -1.2786941780806984 1102 17 -1.3952078582043228 1102 18 -1.5842453210590364 1102 19 -1.6162873673785825 1102 20 -1.6143614145265772 1102 21 -1.7390189607982975 1102 22 -1.8168704008608714 1102 23 -2.087706721166448 1102 24 -1.9463734556078478 1102 25 -1.9911284847291313 1103 1 0.8765723373105914 1103 2 0.01321553005830367 1103 3 -0.49933824263003146 1103 4 -0.5560536154684357 1103 5 -0.4806681341031549 1103 6 -0.7170255573043178 1103 7 -0.8548694923437878 1103 8 -0.7003136810818091 1103 9 -0.9812833894004456 1103 10 -1.0480311596985317 1103 11 -0.9459129005061726 1103 12 -1.2182642395802716 1103 13 -1.2278250174157535 1103 14 -1.4963770117278088 1103 15 -1.373367220322585 1103 16 -1.4851604757604662 1103 17 -1.5675454789774008 1103 18 -1.421408777181604 1103 19 -1.6849054271461057 1103 20 -1.6994104324887687 1103 21 -1.6485403297352121 1103 22 -1.87032873913666 1103 23 -1.9260086204865914 1103 24 -2.0457824499059356 1103 25 -1.926033556023576 1104 1 1.0836041317349014 1104 2 0.34796397673081003 1104 3 -0.12438952712163953 1104 4 -0.16613214534746135 1104 5 -0.3710642196929115 1104 6 -0.47641173317973085 1104 7 -0.4508945566031173 1104 8 -0.5173662602060745 1104 9 -0.6946120817323518 1104 10 -0.8408624878127 1104 11 -0.8212414153251189 1104 12 -0.6888211490582847 1104 13 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-0.8659361104245633 2314 17 -1.0692550821518836 2314 18 -1.9172600580702905 2314 19 -1.6976060656762044 2314 20 -2.098408422162386 2314 21 -1.3759538849501212 2314 22 -0.9306664559523838 2314 23 -0.8030673283226619 2314 24 -0.9055316320378992 2314 25 -0.7205818031060502 2315 1 -2.295796574131167 2315 2 -0.9923319026414461 2315 3 -0.46804326828869947 2315 4 -0.4551304767969526 2315 5 -0.27699564764920837 2315 6 -0.3013408005599558 2315 7 -0.23782296271447478 2315 8 -0.21617905191406805 2315 9 -0.25639304795381435 2315 10 -0.2597624747411089 2315 11 -0.4586676953801714 2315 12 -0.4544443949657264 2315 13 -0.47366945994893683 2315 14 -0.4448303244195273 2315 15 -0.9881947768719925 2315 16 -0.8665600915971723 2315 17 -0.7259985049800974 2315 18 -0.3798503356231606 2315 19 -0.3561447838159211 2315 20 -0.5113166278386672 2315 21 -0.5621895943778483 2315 22 -0.35549831569696266 2315 23 -0.3985839480288078 2315 24 -0.3296963285217984 2315 25 -0.17876426901919018 2316 1 1.4085955384450877 2316 2 0.5934012048307906 2316 3 -0.02481411892144436 2316 4 -0.12899701946144404 2316 5 -0.3361989337067115 2316 6 -0.40347098069525344 2316 7 -0.38924621976433116 2316 8 -0.37773173622890255 2316 9 -0.5323631988600255 2316 10 -0.5716290395488457 2316 11 -0.5605830860423637 2316 12 -0.5045855581503673 2316 13 -0.6878743513916774 2316 14 -0.6681726257669425 2316 15 -0.6452645613075063 2316 16 -0.573033669170492 2316 17 -0.5004031284895802 2316 18 -0.5033277107629388 2316 19 -0.7046351681119347 2316 20 -0.5645257375695181 2316 21 -0.5959358992239032 2316 22 -0.7175381513940877 2316 23 -0.6217169957142801 2316 24 -0.6376236101063668 2316 25 -0.6472765751274068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64061.0 3 3.0 4 502.0 5 2474.0 6 2980.0 7 2749.0 8 2554.0 9 2529.0 10 2433.0 11 2369.0 12 2365.0 13 2168.0 14 2250.0 15 2370.0 16 2504.0 17 2644.0 18 2959.0 19 3533.0 20 4302.0 21 5288.0 22 6379.0 23 7702.0 24 8986.0 25 10420.0 26 11692.0 27 12857.0 28 14851.0 29 18178.0 30 22428.0 31 28907.0 32 37557.0 33 50198.0 34 68954.0 35 97937.0 36 167182.0 37 282113.0 38 741889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.869198720368416 19.6359396232825 11.846854710856107 24.648006945492977 2 14.31185478280144 19.904935837880412 42.93273470696705 22.8504746723511 3 18.989271125199295 24.775882319644523 29.476398362248702 26.75844819290748 4 11.829243271797678 16.535447462007944 39.06802724296994 32.56728202322444 5 12.52735929526346 38.327476837810735 36.28828459208197 12.856879274843832 6 31.48220273718832 37.60584700287483 18.196522037329796 12.715428222607054 7 27.88465395241949 31.395090944697035 22.463304776218713 18.256950326664764 8 24.38304616643691 38.36186920472596 20.549188263637724 16.705896365199404 9 26.7216887314772 14.652350322206148 20.352703025231378 38.273257921085275 10 14.903507386887185 28.786177365418197 35.359316857279545 20.950998390415073 11 34.38411117370184 21.609847904533165 25.821110464252968 18.184930457512028 12 24.03396352044499 24.144798673042192 32.5996765098417 19.221561296671116 13 29.687456720537998 22.208706752214876 25.688508098379266 22.415328428867866 14 20.170242104425764 22.922047501613147 27.817701289632556 29.090009104328534 15 22.200805562616893 32.979702865474344 23.15800835289045 21.661483219018308 16 21.149961553464287 27.845323951159035 29.663139892869005 21.34157460250767 17 20.950672116391967 29.16746100839592 28.684127429136357 21.197739446075754 18 20.796862734020905 26.935280942780437 31.88374838317109 20.384107940027565 19 23.567491532107365 26.32235965074909 28.001341914523575 22.10880690261997 20 23.652522344840502 27.20955829740472 29.010631650670803 20.12728770708398 21 22.232822797833478 26.648904813309137 27.590353711762106 23.52791867709528 22 23.03104971439066 29.01513046672286 27.696689003687258 20.25713081519922 23 21.33632284353096 28.51527213479902 28.87837387930196 21.270031142368058 24 23.20070166456588 26.499280109614443 29.65452929313901 20.645488932680664 25 22.10272090860598 27.776020694766355 29.185686434637297 20.93557196199036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 666.0 1 666.0 2 1084.0 3 1502.0 4 1502.0 5 1502.0 6 2511.5 7 3521.0 8 3521.0 9 3521.0 10 4183.0 11 4845.0 12 4845.0 13 4845.0 14 6525.0 15 8205.0 16 8205.0 17 8205.0 18 14360.0 19 20515.0 20 20515.0 21 20515.0 22 32720.5 23 44926.0 24 44926.0 25 44926.0 26 66989.0 27 89052.0 28 89052.0 29 89052.0 30 116550.5 31 144049.0 32 144049.0 33 144049.0 34 159852.5 35 175656.0 36 175656.0 37 175656.0 38 184934.0 39 194212.0 40 194212.0 41 194212.0 42 202208.0 43 210204.0 44 210204.0 45 210204.0 46 214399.0 47 218594.0 48 218594.0 49 218594.0 50 207644.0 51 196694.0 52 196694.0 53 196694.0 54 172623.0 55 148552.0 56 148552.0 57 148552.0 58 127566.0 59 106580.0 60 106580.0 61 106580.0 62 87456.5 63 68333.0 64 68333.0 65 68333.0 66 52725.5 67 37118.0 68 37118.0 69 37118.0 70 27133.0 71 17148.0 72 17148.0 73 17148.0 74 12082.0 75 7016.0 76 7016.0 77 7016.0 78 4738.5 79 2461.0 80 2461.0 81 2461.0 82 1655.5 83 850.0 84 850.0 85 850.0 86 598.5 87 347.0 88 347.0 89 347.0 90 232.5 91 118.0 92 118.0 93 118.0 94 86.5 95 55.0 96 55.0 97 55.0 98 51.5 99 48.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33968800899564855 2 0.2903130431613615 3 0.3105332672649267 4 0.3011285118679196 5 0.28566944518408927 6 0.24240757035785682 7 0.3497981210474311 8 0.30994547005261375 9 0.25698494122321774 10 0.23129820304514226 11 0.24540533614065282 12 0.24405340255233307 13 0.26133464059433353 14 0.25110696910008834 15 0.34127506146889347 16 0.39417681057705817 17 0.41098781084920827 18 0.11479679556471735 19 0.1294329461513096 20 0.18950582124969215 21 0.1907989751167806 22 0.14359885896805144 23 0.24816798303852364 24 0.3450369636276963 25 0.38436059713143206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1701267.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.9831810962086 #Duplication Level Percentage of deduplicated Percentage of total 1 85.45161639284375 59.801759449841306 2 9.97228798742661 13.95784872335244 3 2.3855748918165065 5.008503590176884 4 0.8143594282518744 2.2796585337902324 5 0.3915597717458795 1.3701299208040993 6 0.2294659961757207 0.9635256219472421 7 0.14087173804459757 0.6901056648438912 8 0.09913301424815195 0.5550114950993156 9 0.0690718542558217 0.4350481276532487 >10 0.38700873592477275 4.956071784142357 >50 0.030401468779154135 1.4989118877285263 >100 0.023761971976349442 3.4271000049031124 >500 0.0027803913940559893 1.4127143699251676 >1k 0.0017693399780356295 2.3680463212389773 >5k 3.3701713867345326E-4 1.2755645045532433 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5716 0.335984886558077 No Hit GTATCAACGCAGAGTACTTTTTTTT 5630 0.3309298305321857 No Hit CTGTAGGACGTGGAATATGGCAAGA 5233 0.30759428120336196 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5054 0.29707271110296035 No Hit CTTTAGGACGTGAAATATGGCGAGG 4297 0.2525764621308707 No Hit GGTATCAACGCAGAGTACTTTTTTT 3303 0.19414941922696438 No Hit GTCCTACAGTGTGCATTTCTCATTT 3295 0.19367918145711402 No Hit TATCAACGCAGAGTACTTTTTTTTT 3264 0.1918570100989439 No Hit CTGTAGGACCTGGAATATGGCGAGA 2372 0.13942549876062957 No Hit CTGAAGGACCTGGAATATGGCGAGA 2300 0.13519335883197642 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2101 0.12349619430694887 No Hit ATTTAGAAATGTCCACTGTAGGACG 2020 0.11873503688721405 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1721 0.10115990023905712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 5.877972123129409E-5 13 0.0 0.0 0.0 0.0 5.877972123129409E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACGG 80 1.2875171E-7 14.251019 5 GTGCGTT 40 0.005301722 14.240925 11 TAGGACC 955 0.0 14.226148 4 ATACCGA 50 0.00149917 13.300558 6 GGTATCA 1000 0.0 13.016315 1 AATGTCC 775 0.0 11.868738 8 GTCCTAG 250 0.0 11.401152 1 GTCTTGT 175 1.8189894E-12 11.401152 1 TAATACT 235 0.0 11.319959 4 AGGACCT 1775 0.0 11.24024 5 CCACCTT 665 0.0 11.144641 13 AAATGTC 810 0.0 11.143101 7 GTATTAG 180 3.6379788E-12 11.084453 1 TGTAGGA 3105 0.0 11.076476 2 GGACCTG 1775 0.0 10.865245 6 GTCCTAA 1325 0.0 10.827509 1 GTCCTAC 2705 0.0 10.818098 1 CACCTTT 695 0.0 10.807952 14 TGTCCAC 860 0.0 10.707345 10 ACCTTTT 720 0.0 10.696794 15 >>END_MODULE