FastQCFastQC Report
Thu 26 May 2016
SRR1512294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701267
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64740.38053991525139796No Hit
GTCCTACAGTGGACATTTCTAAATT58860.34597743916739704No Hit
CTGTAGGACGTGGAATATGGCAAGA58470.34368503003937656No Hit
GTCCTAAAGTGTGTATTTCTCATTT51590.3032445818322462No Hit
CTTTAGGACGTGAAATATGGCGAGG45770.269034784075633No Hit
GGTATCAACGCAGAGTACTTTTTTT40750.23952736401752342No Hit
TATCAACGCAGAGTACTTTTTTTTT39260.2307691855540606No Hit
GTCCTACAGTGTGCATTTCTCATTT33560.19726474445222295No Hit
CTGAAGGACCTGGAATATGGCGAGA27100.15929304453680698No Hit
CTGTAGGACCTGGAATATGGCGAGA26080.153297512971215No Hit
ATTTAGAAATGTCCACTGTAGGACG21480.1262588412048197No Hit
GTCCTTCAGTGTGCATTTCTCATTT21130.12420155096172442No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20920.12296717681586725No Hit
TTTCTAAATTTTCCACCTTTTTCAG18970.1115051311757649No Hit
GAATATGGCAAGAAAACTGAAAATC17800.10462790379170347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1101.2732926E-1114.67266618
TAGGACC9300.014.5078474
GGTATCA9500.014.0176421
AGGACCG1001.437329E-712.3535565
CCAACGA1001.4544821E-712.34229919
AGGCGTG1353.6743586E-1011.9692267
TAGGACT2900.011.7951124
AGGACCT18650.011.7701455
TAGAAAT6050.011.6218154
ATAGGAC2850.011.3352633
TGTAGGA29750.011.3061272
CACCTTT7150.011.28766114
GGACCTG18100.011.185416
GTCCGGT600.005821219611.09731
AGACCGT600.005853198511.0891346
GGCGAGG13500.011.04126119
ACCTTTT7700.010.97399515
CCACCTT7100.010.9701513
GTAGGAC29000.010.9104793
GGCGTGC1053.4445657E-610.8628268
Produced by FastQC (version 0.11.5)