FastQCFastQC Report
Thu 26 May 2016
SRR1512293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725979
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71930.4167489870965985No Hit
GGTATCAACGCAGAGTACTTTTTTT44990.26066365813257286No Hit
TATCAACGCAGAGTACTTTTTTTTT41720.24171788880397735No Hit
GTCCTACAGTGGACATTTCTAAATT40970.23737252886622603No Hit
GTCCTAAAGTGTGTATTTCTCATTT38650.22393088212544882No Hit
CTGTAGGACGTGGAATATGGCAAGA37720.21854263580263722No Hit
CTTTAGGACGTGAAATATGGCGAGG32850.19032676527350564No Hit
GTCCTACAGTGTGCATTTCTCATTT22530.13053461253004817No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21620.1252622424722433No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20360.11796203777682115No Hit
CTGAAGGACCTGGAATATGGCGAGA17430.10098616495333954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.014.1003081
TAGGACC6550.012.1838044
AACCGGG1251.8055289E-811.41119414
CTAGGAC2200.011.2288043
GGCGAGG9500.011.10701419
CGTGGTA600.005917366611.07291310
GGCGTGC600.005955913611.0632588
GCATTAG951.35913615E-511.0008591
TATGTCG1158.705938E-710.74967516
TGGCGAG19200.010.69020418
GTCCTAA11250.010.556381
AGGACCT14500.010.4832735
CCCCGGT1002.3821105E-510.45660319
CTATACA2200.010.3641444
GTCCTAC21950.010.3447711
TTAGGAC13150.010.3322083
TCCTATA2400.010.2930712
GACGTGA11400.010.2505037
TCCTACA23950.010.2352172
GAACAGT2900.010.1556716
Produced by FastQC (version 0.11.5)