##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512293_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1725979 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.596150358723946 31.0 30.0 34.0 16.0 34.0 2 29.497691455110402 31.0 30.0 34.0 16.0 34.0 3 30.143569533580653 31.0 30.0 34.0 25.0 34.0 4 33.78242493101017 37.0 35.0 37.0 29.0 37.0 5 33.94951676700585 37.0 35.0 37.0 30.0 37.0 6 34.100552208340886 37.0 35.0 37.0 30.0 37.0 7 34.03411397241797 37.0 35.0 37.0 30.0 37.0 8 34.021619614143624 37.0 35.0 37.0 30.0 37.0 9 35.545367006203435 39.0 37.0 39.0 30.0 39.0 10 35.46544366994036 39.0 37.0 39.0 30.0 39.0 11 35.49067340912028 39.0 37.0 39.0 30.0 39.0 12 35.42404281859744 39.0 37.0 39.0 30.0 39.0 13 35.39027241930522 39.0 37.0 39.0 30.0 39.0 14 36.55498763310562 40.0 37.0 41.0 30.0 41.0 15 36.47357992188781 40.0 37.0 41.0 29.0 41.0 16 36.3921884333471 40.0 37.0 41.0 29.0 41.0 17 36.34913113079591 40.0 37.0 41.0 29.0 41.0 18 36.134188770547034 40.0 37.0 41.0 27.0 41.0 19 36.233161585395884 40.0 37.0 41.0 27.0 41.0 20 36.160942282611785 40.0 37.0 41.0 27.0 41.0 21 36.09575319282564 40.0 37.0 41.0 27.0 41.0 22 36.007070190309385 40.0 37.0 41.0 27.0 41.0 23 35.87077536864585 40.0 37.0 41.0 27.0 41.0 24 35.73193764234675 40.0 36.0 41.0 27.0 41.0 25 35.60497781259216 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9917230043399456 1101 2 0.04943311190606181 1101 3 -0.3910116221751103 1101 4 -0.5239985624820065 1101 5 -0.6218645808601337 1101 6 -0.7157178197010126 1101 7 -0.8623730609468367 1101 8 -0.8068832874895335 1101 9 -1.2184750716400927 1101 10 -1.1692226839552475 1101 11 -1.1355323821917835 1101 12 -1.1887142351432018 1101 13 -1.2524491533472286 1101 14 -1.3284749521412849 1101 15 -1.6230043131643725 1101 16 -1.695336584833143 1101 17 -1.8525291942970625 1101 18 -1.7534230117470102 1101 19 -1.6436448529245098 1101 20 -2.0086430265881603 1101 21 -1.8768494022059627 1101 22 -1.967139939724703 1101 23 -2.2404706015501716 1101 24 -2.312521253854875 1101 25 -2.3759226899918744 1102 1 1.395846675498607 1102 2 0.41841983080069767 1102 3 -0.1290435241112462 1102 4 -0.004901898511192826 1102 5 -0.28009941834604746 1102 6 -0.2527824648074031 1102 7 -0.2999901922410757 1102 8 -0.43137456821163056 1102 9 -0.29148379903801214 1102 10 -0.49156150967037604 1102 11 -0.5650977012598659 1102 12 -0.7617668106512738 1102 13 -0.5720271721272141 1102 14 -1.08876220598286 1102 15 -1.079479379829742 1102 16 -1.1507528008386743 1102 17 -1.3505690213731043 1102 18 -0.9963025658604536 1102 19 -1.0992267173461485 1102 20 -1.3927881995608402 1102 21 -1.2417014067141992 1102 22 -1.2403104179289457 1102 23 -1.410175779849574 1102 24 -1.5727971396678981 1102 25 -1.437027467487077 1103 1 0.9689843689112152 1103 2 0.182551905929337 1103 3 -0.3484929686466032 1103 4 -0.4040970353924678 1103 5 -0.6179661674384533 1103 6 -0.5808460053566264 1103 7 -0.7317192539815878 1103 8 -0.687494376450573 1103 9 -0.9292741831056048 1103 10 -1.0150043601464418 1103 11 -0.9009947175692545 1103 12 -0.9607583074663637 1103 13 -0.993201496508874 1103 14 -1.2235373837133707 1103 15 -1.3115805695483402 1103 16 -1.4473854668871482 1103 17 -1.4152550459674913 1103 18 -1.2047972657640855 1103 19 -1.6677916003440245 1103 20 -1.4032308136689764 1103 21 -1.4937575047038578 1103 22 -1.6304144804270493 1103 23 -1.8418230219206464 1103 24 -1.8627418053284899 1103 25 -1.9688644099769377 1104 1 1.3719718678348833 1104 2 0.46327686920367483 1104 3 -0.0654359584156019 1104 4 -0.08614347019821622 1104 5 -0.28206529932990065 1104 6 -0.3519211866365808 1104 7 -0.30851894191130924 1104 8 -0.36696283934787033 1104 9 -0.2869534343456692 1104 10 -0.5783270997895471 1104 11 -0.46463187585099064 1104 12 -0.4963169680690598 1104 13 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1108 21 -1.7030782122365196 1108 22 -1.9334809055406978 1108 23 -1.8928340498257867 1108 24 -2.0046764981515963 1108 25 -1.88055625808704 1109 1 0.6081000798518801 1109 2 -0.19453135136978617 1109 3 -0.5840956109874433 1109 4 -0.5483654646441565 1109 5 -0.6022477354015123 1109 6 -0.6925477822200747 1109 7 -0.6280854270501735 1109 8 -0.6471536663582853 1109 9 -0.7719038590494023 1109 10 -0.8715372514248898 1109 11 -0.8719346028424368 1109 12 -0.7273297632957707 1109 13 -0.8827854181068773 1109 14 -1.1410314626493658 1109 15 -1.0831174594258357 1109 16 -1.1184320241541954 1109 17 -1.1217866886841819 1109 18 -0.9742641042840745 1109 19 -1.1746612511373087 1109 20 -1.2489770918344476 1109 21 -1.507385716308825 1109 22 -1.5452568062863037 1109 23 -1.683633769412154 1109 24 -1.879106288036546 1109 25 -1.8102301572539545 1110 1 0.24682369552450467 1110 2 -0.2124039396030959 1110 3 -0.5083722844175966 1110 4 -0.4161836500670404 1110 5 -0.3641072373694527 1110 6 -0.40398770047826105 1110 7 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-1.2924691891746676 2314 18 -2.1451712754355654 2314 19 -1.9634044821861707 2314 20 -2.27023196147848 2314 21 -1.581516498552837 2314 22 -1.3039645029222484 2314 23 -1.2026124604393118 2314 24 -1.1716058473802136 2314 25 -0.9105189829211966 2315 1 -2.6666803618711974 2315 2 -1.2043547856846075 2315 3 -0.654283948722739 2315 4 -0.5772012119006504 2315 5 -0.5652202684564145 2315 6 -0.6488535883418365 2315 7 -0.560691444556582 2315 8 -0.5248878179093666 2315 9 -0.5483526775001835 2315 10 -0.45810727076540303 2315 11 -0.7216815087870714 2315 12 -0.7231879502694767 2315 13 -0.7261096763145432 2315 14 -0.7906356877305569 2315 15 -1.2899770892792333 2315 16 -1.2267070120130157 2315 17 -1.0819201688668088 2315 18 -0.9108145621334529 2315 19 -0.8503498080918845 2315 20 -0.7736130195890141 2315 21 -0.884580734079897 2315 22 -0.7451410946673818 2315 23 -0.7774957802932647 2315 24 -0.6429556479815446 2315 25 -0.5139300664022528 2316 1 0.932719614690388 2316 2 0.25785545690299116 2316 3 -0.4062906677352025 2316 4 -0.4644944655433676 2316 5 -0.6846432927933037 2316 6 -0.8794739250737464 2316 7 -0.862961845744131 2316 8 -0.8304173307809677 2316 9 -0.9603593965126436 2316 10 -0.9540426588664985 2316 11 -1.0030710161897076 2316 12 -0.9057286745165172 2316 13 -0.9552334702127396 2316 14 -1.0418072178684525 2316 15 -1.0795466037133892 2316 16 -1.069278987480807 2316 17 -0.9642515099348188 2316 18 -0.7971046515900753 2316 19 -1.1299306510935665 2316 20 -0.9820195979709823 2316 21 -1.0031205207903184 2316 22 -1.1387635017926598 2316 23 -0.9424365982939662 2316 24 -0.941780602081316 2316 25 -0.9236534930986124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60582.0 3 6.0 4 507.0 5 2320.0 6 2837.0 7 2742.0 8 2527.0 9 2285.0 10 2339.0 11 2200.0 12 2308.0 13 2392.0 14 2307.0 15 2440.0 16 2470.0 17 2658.0 18 2988.0 19 3486.0 20 4315.0 21 5251.0 22 6399.0 23 7588.0 24 8932.0 25 10402.0 26 11419.0 27 13217.0 28 14816.0 29 17931.0 30 22748.0 31 29048.0 32 37771.0 33 50618.0 34 69674.0 35 98624.0 36 166261.0 37 284859.0 38 766712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16888019175551 19.403967311335865 12.116164866487956 24.310987630420666 2 14.734511166091618 19.982638142397448 41.86340145983705 23.419449231673887 3 18.81640407736509 25.018234695496567 29.513704645008982 26.651656582129355 4 12.259198263147557 16.778222399399354 38.379278643711636 32.58330069374145 5 13.022750448123691 37.99560156066157 35.649205579103636 13.332442412111106 6 31.558604331321465 37.02276243710013 18.257923177355583 13.160710054222829 7 27.735670463689516 31.62376216900085 22.417125208727725 18.223442158581907 8 25.008122086437595 37.067284584541476 20.603364955710653 17.32122837331027 9 26.877033434735203 14.779443670263653 20.215354570606547 38.12816832439459 10 15.528154823363804 28.539547297524877 34.35825724994106 21.574040629170263 11 34.24628971714556 21.885746578083353 25.202277425136586 18.665686279634492 12 23.954261101782873 24.281540017179964 31.899327030915224 19.864871850121936 13 29.499297390964895 22.003019563261244 25.69783106130271 22.79985198447115 14 20.94467827058751 22.489286141628554 27.62008873130956 28.945946856474375 15 22.925855711145566 31.457067795890865 23.39657895761863 22.220497535344936 16 21.926630479040103 27.605648713229737 28.445347454728626 22.022373353001534 17 21.559336272425707 28.488077826607512 28.063154416376072 21.88943148459071 18 21.455895361146155 26.649265526892012 30.675425240353242 21.21941387160859 19 23.662032785439678 26.27511018109903 27.893265450444467 22.169591583016825 20 23.89051592772658 26.732980190116827 28.5051012263261 20.87140265583049 21 23.03024920534307 26.169637487839253 27.479515381168312 23.320597925649366 22 23.529981746255547 27.840847772126427 27.589844280358015 21.039326201260007 23 22.19381428449459 27.572149353474185 28.399509566189106 21.834526795842116 24 23.426886073947635 26.22073594150793 28.883483292132457 21.468894692411983 25 22.664087364365614 27.069413166074185 28.656486720393083 21.610012749167115 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 793.0 1 793.0 2 1258.0 3 1723.0 4 1723.0 5 1723.0 6 2881.0 7 4039.0 8 4039.0 9 4039.0 10 4605.5 11 5172.0 12 5172.0 13 5172.0 14 6747.0 15 8322.0 16 8322.0 17 8322.0 18 14222.0 19 20122.0 20 20122.0 21 20122.0 22 31987.5 23 43853.0 24 43853.0 25 43853.0 26 64435.5 27 85018.0 28 85018.0 29 85018.0 30 110266.5 31 135515.0 32 135515.0 33 135515.0 34 150126.5 35 164738.0 36 164738.0 37 164738.0 38 175071.0 39 185404.0 40 185404.0 41 185404.0 42 195237.0 43 205070.0 44 205070.0 45 205070.0 46 213065.0 47 221060.0 48 221060.0 49 221060.0 50 213215.0 51 205370.0 52 205370.0 53 205370.0 54 183593.0 55 161816.0 56 161816.0 57 161816.0 58 140309.5 59 118803.0 60 118803.0 61 118803.0 62 99347.0 63 79891.0 64 79891.0 65 79891.0 66 61942.5 67 43994.0 68 43994.0 69 43994.0 70 32544.0 71 21094.0 72 21094.0 73 21094.0 74 14975.5 75 8857.0 76 8857.0 77 8857.0 78 6147.0 79 3437.0 80 3437.0 81 3437.0 82 2344.0 83 1251.0 84 1251.0 85 1251.0 86 825.0 87 399.0 88 399.0 89 399.0 90 267.0 91 135.0 92 135.0 93 135.0 94 99.0 95 63.0 96 63.0 97 63.0 98 51.5 99 40.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3402127140596728 2 0.2876628278791341 3 0.31043251395295074 4 0.2992504543798042 5 0.2847079831214632 6 0.24305046585155438 7 0.3496566296577189 8 0.31066426648296414 9 0.2575929371098953 10 0.22972469537578386 11 0.24391953783910464 12 0.24368778530909127 13 0.2629232453002035 14 0.2535952059671642 15 0.3395174564696326 16 0.3934578578302517 17 0.41025991625622327 18 0.11321111091154644 19 0.12897028295245772 20 0.19432449641623684 21 0.18673460105829792 22 0.14467151686086563 23 0.24554180554919847 24 0.34478982652743745 25 0.3853465192797827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1725979.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.77213411387416 #Duplication Level Percentage of deduplicated Percentage of total 1 85.11260699620847 60.2360083711713 2 10.210740806943527 14.452718355820299 3 2.426066429039037 5.150936960553554 4 0.894593745566739 2.532492341547291 5 0.4154241382433546 1.4700226412949646 6 0.22921943186454907 0.9733409024054346 7 0.13937596325720178 0.6904754054722356 8 0.09759144236900108 0.5525403718164292 9 0.07161583489187416 0.4561564924480308 >10 0.3460149772151662 4.379423874403353 >50 0.029948691477823815 1.4824711962726347 >100 0.022033763126130076 3.1073739677993597 >500 0.0032059208980890243 1.5993990088296703 >1k 0.0014796557991180112 2.498173753752178 >5k 8.220309995100061E-5 0.41846635641320457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7193 0.4167489870965985 No Hit GGTATCAACGCAGAGTACTTTTTTT 4499 0.26066365813257286 No Hit TATCAACGCAGAGTACTTTTTTTTT 4172 0.24171788880397735 No Hit GTCCTACAGTGGACATTTCTAAATT 4097 0.23737252886622603 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3865 0.22393088212544882 No Hit CTGTAGGACGTGGAATATGGCAAGA 3772 0.21854263580263722 No Hit CTTTAGGACGTGAAATATGGCGAGG 3285 0.19032676527350564 No Hit GTCCTACAGTGTGCATTTCTCATTT 2253 0.13053461253004817 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2162 0.1252622424722433 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2036 0.11796203777682115 No Hit CTGAAGGACCTGGAATATGGCGAGA 1743 0.10098616495333954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.793813250335027E-5 2 0.0 0.0 0.0 0.0 5.793813250335027E-5 3 0.0 0.0 0.0 0.0 5.793813250335027E-5 4 0.0 0.0 0.0 0.0 5.793813250335027E-5 5 0.0 0.0 0.0 0.0 5.793813250335027E-5 6 2.8969066251675137E-4 0.0 0.0 0.0 5.793813250335027E-5 7 2.8969066251675137E-4 0.0 0.0 0.0 5.793813250335027E-5 8 2.8969066251675137E-4 0.0 0.0 5.793813250335027E-5 5.793813250335027E-5 9 2.8969066251675137E-4 0.0 0.0 5.793813250335027E-5 5.793813250335027E-5 10 2.8969066251675137E-4 0.0 0.0 5.793813250335027E-5 5.793813250335027E-5 11 2.8969066251675137E-4 0.0 0.0 1.738143975100508E-4 5.793813250335027E-5 12 2.8969066251675137E-4 0.0 0.0 2.317525300134011E-4 5.793813250335027E-5 13 2.8969066251675137E-4 0.0 0.0 2.317525300134011E-4 5.793813250335027E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1260 0.0 14.100308 1 TAGGACC 655 0.0 12.183804 4 AACCGGG 125 1.8055289E-8 11.411194 14 CTAGGAC 220 0.0 11.228804 3 GGCGAGG 950 0.0 11.107014 19 CGTGGTA 60 0.0059173666 11.072913 10 GGCGTGC 60 0.0059559136 11.063258 8 GCATTAG 95 1.35913615E-5 11.000859 1 TATGTCG 115 8.705938E-7 10.749675 16 TGGCGAG 1920 0.0 10.690204 18 GTCCTAA 1125 0.0 10.55638 1 AGGACCT 1450 0.0 10.483273 5 CCCCGGT 100 2.3821105E-5 10.456603 19 CTATACA 220 0.0 10.364144 4 GTCCTAC 2195 0.0 10.344771 1 TTAGGAC 1315 0.0 10.332208 3 TCCTATA 240 0.0 10.293071 2 GACGTGA 1140 0.0 10.250503 7 TCCTACA 2395 0.0 10.235217 2 GAACAGT 290 0.0 10.155671 6 >>END_MODULE