FastQCFastQC Report
Thu 26 May 2016
SRR1512293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725979
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74560.4319867159449796No Hit
GGTATCAACGCAGAGTACTTTTTTT49080.28436035432644313No Hit
TATCAACGCAGAGTACTTTTTTTTT47750.2766545827034976No Hit
GTCCTACAGTGGACATTTCTAAATT41570.2408488168164271No Hit
CTGTAGGACGTGGAATATGGCAAGA41050.23783603392625285No Hit
GTCCTAAAGTGTGTATTTCTCATTT39880.23105727242336088No Hit
CTTTAGGACGTGAAATATGGCGAGG36190.20967810152962463No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23060.13360533355272572No Hit
GTCCTACAGTGTGCATTTCTCATTT22960.13302595222769223No Hit
CTGAAGGACCTGGAATATGGCGAGA19600.11355873970656652No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19290.11176265759896267No Hit
CTGTAGGACCTGGAATATGGCGAGA17370.10063853615831941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.014.0601161
AAGACCG801.99641E-613.0644535
TAGGACC7100.012.1775244
GTGACGT550.003058597212.0962239
GTGGTAT3900.011.9470351
GCGTGCG1052.7048736E-711.7670769
GGCGAGG11000.011.39344719
GTCCTAT2550.011.1868991
ATGACCG600.005869567411.084995
CGCCCTA1658.185452E-1110.93310516
ACTGTTC4600.010.7444748
AGACCGT803.7538295E-410.6928196
ACCGTGC909.4676696E-510.5608088
AGGACGT23300.010.5208855
AAATGTC5800.010.4879757
TTAGGAC14500.010.4184973
AGGCGTG1106.009517E-610.36879357
TCCCGCC1856.002665E-1110.26882913
GGACGTG23050.010.1851096
TGGTATC4200.010.1797982
Produced by FastQC (version 0.11.5)