FastQCFastQC Report
Thu 26 May 2016
SRR1512292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741746
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62450.3585482613423542No Hit
GTCCTACAGTGGACATTTCTAAATT41660.23918527730220135No Hit
GGTATCAACGCAGAGTACTTTTTTT38900.22333910914679866No Hit
TATCAACGCAGAGTACTTTTTTTTT37810.21708102099846938No Hit
GTCCTAAAGTGTGTATTTCTCATTT37800.2170236073457324No Hit
CTGTAGGACGTGGAATATGGCAAGA37720.21656429812383662No Hit
CTTTAGGACGTGAAATATGGCGAGG30890.1773507733044887No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18920.10862663097834012No Hit
GTCCTACAGTGTGCATTTCTCATTT18440.10587077564696575No Hit
CTGAAGGACCTGGAATATGGCGAGA18260.10483732989770035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11150.014.4853171
TACAGCG701.0906226E-412.2147875
CTGTCGG550.00310574112.0715489
TAGGACC7000.011.6719074
AGGACCT14550.011.4265855
TATGTCG752.0533674E-411.41232316
GGCGAGG9300.011.04067519
GGACCTG14450.010.9796896
GCGCCAC1053.4622626E-610.85821513
GTCTTAG2550.010.8046761
TGGCGAG21950.010.65012718
TCCAACG1355.6617864E-810.5587318
TGTAGGA23350.010.4568332
TAGAAAT6100.010.43485454
CACCTTT6050.010.37364114
GTTTAGG2200.010.364361
GTAGGAC22150.010.3370653
TAGGACG26200.010.2981324
GACGTGG12950.010.27098757
AGGACGT25900.010.2340115
Produced by FastQC (version 0.11.5)