FastQCFastQC Report
Thu 26 May 2016
SRR1512292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741746
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74080.4253203394754459No Hit
GGTATCAACGCAGAGTACTTTTTTT47270.27139433648763944No Hit
TATCAACGCAGAGTACTTTTTTTTT45690.26232297935519877No Hit
GTCCTACAGTGGACATTTCTAAATT42790.24567302006147854No Hit
CTGTAGGACGTGGAATATGGCAAGA42740.2453859517977937No Hit
GTCCTAAAGTGTGTATTTCTCATTT38010.21822929405320868No Hit
CTTTAGGACGTGAAATATGGCGAGG34280.19681400158232026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21730.12475986739742763No Hit
CTGAAGGACCTGGAATATGGCGAGA20310.11660712870877844No Hit
GTCCTACAGTGTGCATTTCTCATTT18760.10770801253454866No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17930.10294267935738047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.014.5605041
GTACTAG1156.983282E-811.5760531
TAGGACC7600.011.1280624
CGTGCGC600.00588043711.08224510
GGCGAGG10500.010.66931819
GACGTGG12250.010.320097
TGTAGGA24300.010.2456192
GTATCAA26350.010.1404211
GCGCCCA750.002662026810.1268119
GTAATAC856.5771263E-410.0615933
AGGACGT25400.09.9518435
GTAGGAC23800.09.9418123
CTGTAGG24200.09.9018311
GTTTTAG1252.539089E-69.8892561
TTTAGAA7250.09.8302912
GGACGTG24650.09.7942746
ATTTAGA6800.09.7885731
TTAGGAC15150.09.7848713
TGGCGAG21350.09.73845918
CGCCCTA1951.6552804E-109.73731816