FastQCFastQC Report
Thu 26 May 2016
SRR1512291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469305
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53270.3625523631921214No Hit
GGTATCAACGCAGAGTACTTTTTTT35330.24045382000333493No Hit
TATCAACGCAGAGTACTTTTTTTTT32640.2221458444638792No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16920.11515648554929032No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15530.10569623053076115No Hit
GTCCTACAGTGGACATTTCTAAATT15130.10297385498586066No Hit
GTCCTAAAGTGTGTATTTCTCATTT14990.10202102354514549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTCG707.3711344E-613.5518169
GGTATCA9450.013.4730581
GTGCGCC604.113442E-412.65873711
GTATAAA1900.012.0019231
TAGGACC3750.011.9093894
ACTTAGT752.104364E-411.381978
GTAATAC1104.952144E-711.2298393
TAATACT2550.011.1790274
ACAGCGT600.005945004511.06580458
GTGCTAA1553.110472E-1011.0340251
TTACACC1408.3164196E-910.8596264
GTCCTAC9500.010.70171451
TCGAACT909.468226E-510.56049819
AGCGTGG909.587146E-510.54750510
GTGTTAT1451.4386387E-810.4844381
GTTTAGG1553.6488927E-910.4210241
GTATCAA22100.010.3613881
TTAGGAC7350.010.3425013
ATAGGAC1201.5185306E-610.294023
TAGGACA3700.010.2726184
Produced by FastQC (version 0.11.5)