FastQCFastQC Report
Thu 26 May 2016
SRR1512291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469305
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57470.39113730641357647No Hit
GGTATCAACGCAGAGTACTTTTTTT41060.2794518496840343No Hit
TATCAACGCAGAGTACTTTTTTTTT38760.26379819030085655No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18070.12298331524087919No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15230.10365444887208577No Hit
GTCCTACAGTGGACATTTCTAAATT14980.101952964156523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.014.1806121
TCCAACG655.4779972E-513.14720218
TAGGACC4350.012.4508874
AAGTCGT907.433813E-611.6147337
TCGAACT752.0827631E-411.39462919
CGCCCTA1105.0016206E-711.2212216
CCGTTTC600.005879632711.08226113
GTAGGAC10700.010.9228513
ATTGGAC1158.789593E-710.7413843
GGCGAGG4600.010.73407119
GACGTGA4900.010.6665927
TAGGACT1701.382432E-1010.6198744
TGTAGGA11300.010.5110532
TAGGACA3550.010.4388084
TTAGGAC6200.010.4215393
ACAGTCC1659.240466E-1010.3668698
GACTGTC1659.240466E-1010.3668697
CGTGAAA5500.010.3654579
ACGTGAA5850.010.233968
TAGACTG1951.4551915E-1110.2332645
Produced by FastQC (version 0.11.5)