FastQCFastQC Report
Thu 26 May 2016
SRR1512290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894594
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57210.30196443142963614No Hit
GTCCTACAGTGGACATTTCTAAATT40700.21482175072865214No Hit
GTCCTAAAGTGTGTATTTCTCATTT39140.20658779664666943No Hit
CTGTAGGACGTGGAATATGGCAAGA39060.20616554259118314No Hit
GGTATCAACGCAGAGTACTTTTTTT37410.1974565526967783No Hit
CTTTAGGACGTGAAATATGGCGAGG35140.1854750938723547No Hit
TATCAACGCAGAGTACTTTTTTTTT33800.17840233844295927No Hit
CTGAAGGACCTGGAATATGGCGAGA18990.10023255642105906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.012.9054811
TAGGACC5950.011.6572694
GGCGAGG10600.011.38776619
GTCCTAA11300.010.9303181
GTGTAGG2700.010.9085191
AGGACGT24200.010.5615535
GTGGTAT4350.010.4838271
ACCTTTT6900.010.47233515
CACCTTT7050.010.3843814
GGACGTG24100.010.3688276
AGGACCG750.00264461710.1349045
GACGTGA13200.010.0775877
AAATGTC6150.010.0424737
CCACCTT7400.010.015861513
TATACTG3250.09.9400025
TGTAGGA22100.09.9314032
GACTGAT1451.6128251E-79.82937057
GTCCAGA2900.09.8285881
TTAGGAC14050.09.805793
TCAGGAC3200.09.7983933
Produced by FastQC (version 0.11.5)