FastQCFastQC Report
Thu 26 May 2016
SRR1512290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894594
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58010.30618697198449907No Hit
CTGTAGGACGTGGAATATGGCAAGA43550.22986455145535137No Hit
GTCCTACAGTGGACATTTCTAAATT41670.21994158115142348No Hit
GGTATCAACGCAGAGTACTTTTTTT39560.20880463043797245No Hit
GTCCTAAAGTGTGTATTTCTCATTT39510.20854072165329354No Hit
CTTTAGGACGTGAAATATGGCGAGG39270.20727395948683466No Hit
TATCAACGCAGAGTACTTTTTTTTT36790.19418408376675952No Hit
CTGAAGGACCTGGAATATGGCGAGA21510.11353355916887733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.013.2804061
TTAACCC751.4739224E-512.6708934
GTACTAG1001.4220313E-712.363921
TATGTCG1501.4551915E-1112.02559416
TTATACT1950.011.2088674
TAGGACC6700.011.0633924
GGCGAGG10750.011.03969919
ATGTCGA1658.185452E-1110.93235917
CTAGACT1752.0008883E-1110.8607664
ATTATAC1855.456968E-1210.7868123
GGCGTGC909.46677E-510.561038
TAGGACA6450.010.16618254
GTATCGC750.002636660810.1385896
TGTAGGA23300.010.0736442
GTAGGAC23450.010.0497293
GTCCTAA10900.010.0342331
TAGAAAT6550.010.0109744
TTTATAC1909.822543E-1110.0028093
AATGCCG1151.034072E-59.9069318
GACGTGA14050.09.8770077
Produced by FastQC (version 0.11.5)