FastQCFastQC Report
Thu 26 May 2016
SRR1512289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525894
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55480.21964500489727598No Hit
GTCCTACAGTGGACATTTCTAAATT39580.1566969952024907No Hit
GTCCTAAAGTGTGTATTTCTCATTT35410.14018798888631112No Hit
CTGTAGGACGTGGAATATGGCAAGA34940.13832726155571057No Hit
GGTATCAACGCAGAGTACTTTTTTT33690.13337851865517714No Hit
TATCAACGCAGAGTACTTTTTTTTT31310.12395611217256147No Hit
CTTTAGGACGTGAAATATGGCGAGG29480.11671115256618053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.013.1496911
GCACCGT905.393704E-712.6697056
TAGGACC8250.012.0938094
ATCCCGT658.1003626E-411.67953810
ACCGTCC658.149112E-411.67118558
AATGTCC6000.010.9052638
GTATCAA26100.010.4846481
TTAGGAC12550.010.4493453
AGGACGT20400.010.3872965
AGGACCG1659.258656E-1010.3661225
GGCGAGG11200.010.18466219
GGACGTG20800.010.1418556
GTCCTAA11850.010.10308551
TGTAGGA23650.010.0452932
GTAGGAC22550.010.0296123
ATAGGAC2950.09.9860423
ATTATAC3250.09.941443
TGTCCAC8050.09.90221710
GTCCTAC21500.09.81106951
ACGTGAA12200.09.7937518
Produced by FastQC (version 0.11.5)