FastQCFastQC Report
Thu 26 May 2016
SRR1512289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525894
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61110.24193414292127857No Hit
GGTATCAACGCAGAGTACTTTTTTT39730.1572908443505547No Hit
GTCCTACAGTGGACATTTCTAAATT39650.15697412480492054No Hit
CTGTAGGACGTGGAATATGGCAAGA39410.15602396616801814No Hit
TATCAACGCAGAGTACTTTTTTTTT36450.14430534297955494No Hit
GTCCTAAAGTGTGTATTTCTCATTT34940.13832726155571057No Hit
CTTTAGGACGTGAAATATGGCGAGG32530.12878608524348212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.014.3815941
TAGGACC9750.012.0854164
TAGGACT3500.011.4031744
GTCCTAC21200.010.6794921
CTAGGAC3600.010.2937163
ACACCGT1303.818932E-710.2356466
AGACCGT750.002638100410.1381646
TACACCG750.002642006810.1363555
TGGCGAG22250.010.112518
GGCGAGG10050.010.1080319
CTAGAAC3600.010.0297763
TCCTACA24100.010.0140462
GCGAACC951.6460443E-49.99921811
AAGACCG1151.0233187E-59.9160015
CCGGTTT1252.5739446E-69.87922812
GTAGGAC23000.09.8737053
TGTAGGA23200.09.870112
GTATCAA25850.09.7888261
GTCCTAA11500.09.7609851
GGACCTG15650.09.717096
Produced by FastQC (version 0.11.5)