FastQCFastQC Report
Thu 26 May 2016
SRR1512288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862529
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70150.3766384308647006No Hit
GGTATCAACGCAGAGTACTTTTTTT43260.23226483990316393No Hit
TATCAACGCAGAGTACTTTTTTTTT42990.2308151980452385No Hit
GTCCTACAGTGGACATTTCTAAATT32140.17256107153230904No Hit
GTCCTAAAGTGTGTATTTCTCATTT29070.15607810670330502No Hit
CTGTAGGACGTGGAATATGGCAAGA28290.151890252447076No Hit
CTTTAGGACGTGAAATATGGCGAGG24000.1288570540378163No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20490.11001170988478569No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19600.10523326079755001No Hit
GTCCTACAGTGTGCATTTCTCATTT18720.10050850214949672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGT400.00534803314.2223688
GGTATCA12450.013.9676241
TGTACCG1052.714096E-711.7641275
TAGGACC5850.011.3696694
TAGAACG700.001489287610.8591944
GTATAAA2350.010.5134751
TGGCGAG14650.010.31169318
ATTAGAC1303.8288272E-710.2332543
GGCGAGA9150.010.07783719
CTAGGAC2550.010.0612673
TGTAGGA18350.010.0460362
GTATCAA27350.010.0063571
GTGTAGG4000.09.9776931
GACGTGG9250.09.9635067
TGTCGAG1054.100499E-59.95345618
CCGAATG1151.0251641E-59.91411418
GCGATTT1151.0357355E-59.9055611
TTTCCGA1252.5435293E-69.88800515
CTAATAC1356.4213054E-79.8542453
ACGAAAT1356.4903907E-79.84653812
Produced by FastQC (version 0.11.5)