FastQCFastQC Report
Thu 26 May 2016
SRR1512288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862529
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78590.421953161534666No Hit
GGTATCAACGCAGAGTACTTTTTTT51820.2782238558433184No Hit
TATCAACGCAGAGTACTTTTTTTTT50020.2685595767904822No Hit
GTCCTACAGTGGACATTTCTAAATT33090.17766166325463925No Hit
GTCCTAAAGTGTGTATTTCTCATTT30090.16155453149991222No Hit
CTGTAGGACGTGGAATATGGCAAGA29850.16026596095953405No Hit
CTTTAGGACGTGAAATATGGCGAGG26680.1432460917387058No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24790.13309859873322777No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21150.11355527887082564No Hit
GTCCTACAGTGTGCATTTCTCATTT18660.10018635951440219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.014.2371461
ACCGCTC701.0862794E-412.2201098
TAGGACC6800.012.0191434
GTCCTAA8400.011.3246571
GTATTAG1303.2030584E-810.9762061
TGTAGGA19350.010.9520682
CGCTCCA700.001494072610.85502410
GTAGGAC18700.010.8248513
ACTAGAC909.4809184E-510.5594533
GACGTGG8650.010.3286227
TTAGGAC10300.010.2416453
TAGGACA4950.010.1754734
AGGACGT17600.010.1517475
CTGTAGG20100.010.0806361
GTATCAA30750.010.0540871
GGCGAGG7750.010.04533319
GTGGTAT4750.010.0133821
TAGGACG18250.09.9982124
GGACGTG17650.09.9084046
AGGACCT12350.09.6961475
Produced by FastQC (version 0.11.5)