FastQCFastQC Report
Thu 26 May 2016
SRR1512287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441905
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT153223.467261062898134No Hit
GGTATCAACGCAGAGTACTTTTTTT98852.236906122356615No Hit
TATCAACGCAGAGTACTTTTTTTTT89732.0305269232074767No Hit
GTACTGGTTCACTATCGGTCAGTCA63631.439902241431982No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45111.0208076396510561No Hit
GTACTTTTTTTTTTTTTTTTTTTTT42890.9705705977529107No Hit
GGATACCACGTGTCCCGCCCTACTC39110.8850318507371493No Hit
ATCATTAACTGAATCCATAGGTTAA35140.7951935370724478No Hit
GTACATGGGGGTTAAGCGACTAAGC34620.7834263020332424No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31740.7182539233545672No Hit
GTATTTAGCCTTGGAGGATGGTCCC27020.6114436360756271No Hit
ATACAGGGTGACAGCCCCGTACACA26480.5992238150733755No Hit
GGTTAATGAGGCGAACCGGGGGAAC26210.5931139045722498No Hit
GTATCAACGCAGAGTACATGGGGGT24870.5627906450481438No Hit
GTGGATGCCCTGGCAGTCAGAGGCG24540.555322976657879No Hit
GTTCACTATCGGTCAGTCAGGAGTA22610.5116484312239056No Hit
GGTATCAACGCAGAGTACATGGGGG22360.5059911066858261No Hit
GTTAATGATAGTGTGTCGAAACACA21320.4824566366074156No Hit
CTATCGGTCAGTCAGGAGTATTTAG21160.4788359489030448No Hit
CCCATATTCAGACAGGATACCACGT21090.47725189803238255No Hit
AGCGTACACGGTGGATGCCCTGGCA21070.4767993120693361No Hit
TATTCAGACAGGATACCACGTGTCC20730.4691053506975481No Hit
GCCTTGGAGGATGGTCCCCCCATAT20580.4657109559747004No Hit
ACTTAGATGTTTCAGTTCCCCCGGT20010.4528122560278793No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19620.44398682974847536No Hit
GTCAGGAGTATTTAGCCTTGGAGGA18750.4242993403559589No Hit
ACCCTGTATCGCGCGCCTTTCCAGA17700.40053857729602516No Hit
GTGGTATCAACGCAGAGTACATGGG17490.3957864246840384No Hit
TCTAAGTACCCCGAGGAAAAGAAAT17300.39148685803509803No Hit
CGGTACTGGTTCACTATCGGTCAGT15980.36161618447403854No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG15230.3446442108598002No Hit
GAGTACATGGGGGTTAAGCGACTAA15110.34192869508152207No Hit
GGTTCACTATCGGTCAGTCAGGAGT15080.34124981613695254No Hit
GCCCAGAGCCTGAATCAGTGTGTGT14970.3387605933401976No Hit
GTGCTAATCTGCGATAAGCGTCGGT14800.3349136126543035No Hit
TATCAACGCAGAGTACATGGGGGTT14200.32133603376291286No Hit
ACGCAGAGTACATGGGGGTTAAGCG14120.31952568991072744No Hit
GTACTTAGATGTTTCAGTTCCCCCG13980.3163575881694029No Hit
GTTATAACCGGCGATTTCCGAATGG13420.303685181204105No Hit
TCACGGTACTGGTTCACTATCGGTC13320.3014222513888732No Hit
GATATGAACCGTTATAACCGGCGAT13150.2975752707029791No Hit
CATCTAAGTACCCCGAGGAAAAGAA13130.29712268473993275No Hit
GGTGATATGAACCGTTATAACCGGC12610.28535544970072757No Hit
GGAGTATTTAGCCTTGGAGGATGGT12470.28218734795940303No Hit
TCATTAACCTATGGATTCAGTTAAT12220.27653002342132355No Hit
GCCTGAATCAGTGTGTGTGTTAGTG12220.27653002342132355No Hit
ATCAACGCAGAGTACTTTTTTTTTT11780.26657313223430373No Hit
GTGGTATCAACGCAGAGTACTTTTT11480.2597843427886084No Hit
GAATCAGTGTGTGTGTTAGTGGAAG11370.25729511999185345No Hit
CACTATCATTAACTGAATCCATAGG10880.24620676389721774No Hit
GGGTACTTAGATGTTTCAGTTCCCC10000.22629298152317803No Hit
GTCCCGCCCTACTCATCGAGCTCAC9820.22221970785576087No Hit
CTATCATTAACTGAATCCATAGGTT9740.22040936400357541No Hit
TCGGTAAGGTGATATGAACCGTTAT9670.21882531313291317No Hit
CCGTTATAACCGGCGATTTCCGAAT9620.2176938482252973No Hit
ATCCATAGGTTAATGAGGCGAACCG9550.21610979735463506No Hit
GCGTACACGGTGGATGCCCTGGCAG9550.21610979735463506No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG9400.21271540263178737No Hit
CCCCAGTAGCGGCGAGCGAACGGGG9060.2050214412599993No Hit
CCTATGGATTCAGTTAATGATAGTG9000.20366368337086027No Hit
GCCCTACTCATCGAGCTCACAGCAT8820.19959040970344305No Hit
CACACACACTGATTCAGGCTCTGGG8550.19348049920231725No Hit
GGTATCAACGCAGAGTACATGGGGA8280.18737058870119142No Hit
GTACATGGGCATCTAAGTACCCCGA8050.18216585012615832No Hit
GTCCCCCCATATTCAGACAGGATAC7830.17718740453264842No Hit
AGCTCACAGCATGTGCATTTTTGTG7730.17492447471741662No Hit
TTTGTGTACGGGGCTGTCACCCTGT7140.16157318880754915No Hit
TAAGTACCCCGAGGAAAAGAAATCA7090.16044172389993325No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA7090.16044172389993325No Hit
CCCTGGCAGTCAGAGGCGATGAAGG7010.15863138004774782No Hit
GTGTTTCGACACACTATCATTAACT6970.1577262081216551No Hit
AGTCAGGAGTATTTAGCCTTGGAGG6860.15523698532490016No Hit
CATTAACTGAATCCATAGGTTAATG6760.15297405550966836No Hit
GGGAAACCCAGTGTGTTTCGACACA6750.15274776252814518No Hit
GGGTTTCCCCATTCGGAAATCGCCG6730.15229517656509883No Hit
ATGATAGTGTGTCGAAACACACTGG6610.1495796607868207No Hit
GGGTGACAGCCCCGTACACAAAAAT6590.14912707482377432No Hit
TAATGATAGTGTGTCGAAACACACT6490.14686414500854256No Hit
ATTTAGCCTTGGAGGATGGTCCCCC6450.14595897308244984No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA6340.1434697502856949No Hit
TTCCAGACGCTTCCACTAACACACA6220.14075423450741675No Hit
GTGGTATCCTGTCTGAATATGGGGG6150.1391701836367545No Hit
GTATCGCGCGCCTTTCCAGACGCTT6110.13826501171066177No Hit
GATTCAGGCTCTGGGCTGCTCCCCG6070.1373598397845691No Hit
GGGGTTAAGCGACTAAGCGTACACG6040.13668096083999953No Hit
GGTATCAACGCAGAGTACATGGGCA6010.13600208189543No Hit
GTGCATTTTTGTGTACGGGGCTGTC6000.13577578891390685No Hit
GTATCAACGCAGAGTACATGGGGAA5940.13441803102476776No Hit
GGAGGATGGTCCCCCCATATTCAGA5820.13170251524648963No Hit
CCACGTGTCCCGCCCTACTCATCGA5790.1310236363019201No Hit
GTACATGGGATAGGTTAATGAGGCG5760.13034475735735057No Hit
GGGGAAACCCAGTGTGTTTCGACAC5750.1301184643758274No Hit
CAGTAGCGGCGAGCGAACGGGGAGC5740.1298921713943042No Hit
AAGTACCCCGAGGAAAAGAAATCAA5690.12876070648668833No Hit
TCCCCCAGTAGCGGCGAGCGAACGG5300.11993528020728438No Hit
CCCTCACGGTACTGGTTCACTATCG5290.1197089872257612No Hit
AGGTTAATGAGGCGAACCGGGGGAA5290.1197089872257612No Hit
GTCAGAGGCGATGAAGGACGTGCTA5200.1176723503920526No Hit
GTATCAACGCAGAGTACATGGGGAG5160.11676717846595987No Hit
GTATCAACGCAGAGTACATGGGCAT5140.11631459250291352No Hit
GAATATGGGGGGACCATCCTCCAAG4950.11201502585397313No Hit
CATTAACCTATGGATTCAGTTAATG4860.10997838902026454No Hit
CGGTGGATGCCCTGGCAGTCAGAGG4860.10997838902026454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4840.10952580305721817No Hit
GTACGGGGCTGTCACCCTGTATCGC4810.10884692411264865No Hit
CACTGATTCAGGCTCTGGGCTGCTC4770.10794175218655593No Hit
GGTATCAACGCAGAGTACATGGGAT4710.10658399429741687No Hit
GGGCTGTCACCCTGTATCGCGCGCC4640.10499994342675462No Hit
ATTCAGACAGGATACCACGTGTCCC4610.10432106448218507No Hit
GACTAAGCGTACACGGTGGATGCCC4610.10432106448218507No Hit
GAGTAGGGCGGGACACGTGGTATCC4590.10386847851913875No Hit
CAACGCAGAGTACTTTTTTTTTTTT4560.10318959957456919No Hit
CAGTTAATGATAGTGTGTCGAAACA4490.10160554870390695No Hit
ACCCAGTGTGTTTCGACACACTATC4460.10092666975933741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTGC250.00598775319.020615
CTACATG359.888551E-519.0076181
TTAGGAC250.006015363619.0054573
ACACCGT307.7017077E-419.0032986
TAGGACC250.006019316619.0032984
GGACGTT307.7786145E-418.9752510
ATATCAA853.6379788E-1217.8895241
ATACATG453.5174642E-516.8956621
GAGGTTA402.795095E-416.5939249
GGAGGTT402.8023627E-416.5882768
ATTTGCC350.002151242716.3033716
TCGTAAG350.002161077216.29224413
CTTAGGA350.002162719816.2903922
TAGACCC350.002164363616.288544
GACCCGA350.002164363616.288546
ACGTTAG350.002177551916.27373712
GGTATCA21350.015.001331
CGTATCA456.7282084E-414.7837041
CGTAAGG400.005233376814.26544914
AGTATCA400.00525713814.2557141
Produced by FastQC (version 0.11.5)