##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512287_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 441905 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.468428734682792 31.0 30.0 34.0 16.0 34.0 2 30.220717122458446 31.0 30.0 34.0 25.0 34.0 3 30.82987519942069 33.0 31.0 34.0 27.0 34.0 4 34.63724329889909 37.0 35.0 37.0 32.0 37.0 5 34.85119426120999 37.0 35.0 37.0 32.0 37.0 6 35.08483497584322 37.0 35.0 37.0 33.0 37.0 7 35.040112693904796 37.0 35.0 37.0 33.0 37.0 8 35.079170862515696 37.0 35.0 37.0 33.0 37.0 9 36.62471571944196 39.0 37.0 39.0 33.0 39.0 10 36.61034611511524 39.0 37.0 39.0 33.0 39.0 11 36.654762901528606 39.0 37.0 39.0 33.0 39.0 12 36.58377931908442 39.0 37.0 39.0 33.0 39.0 13 36.55562168339349 39.0 37.0 39.0 33.0 39.0 14 37.603842454826264 40.0 38.0 41.0 32.0 41.0 15 37.394521446917324 40.0 38.0 41.0 32.0 41.0 16 37.318704246387796 40.0 38.0 41.0 32.0 41.0 17 37.49528971158959 40.0 38.0 41.0 32.0 41.0 18 37.346760050237044 40.0 38.0 41.0 32.0 41.0 19 37.44741969427818 40.0 38.0 41.0 32.0 41.0 20 37.46857582512079 40.0 38.0 41.0 32.0 41.0 21 37.43635623041151 40.0 38.0 41.0 32.0 41.0 22 37.30743485590794 40.0 38.0 41.0 32.0 41.0 23 37.13170930403594 40.0 38.0 41.0 32.0 41.0 24 36.89300641540603 40.0 37.0 41.0 31.0 41.0 25 36.73235650196309 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0421596701784992 1101 2 0.23315626023191882 1101 3 -0.1420983548235064 1101 4 -0.31393416039963284 1101 5 -0.5820682041192882 1101 6 -0.5600281746898759 1101 7 -0.5143513920294964 1101 8 -0.6969454085372178 1101 9 -1.0400031451010676 1101 10 -1.1595457078015485 1101 11 -1.0112673965555885 1101 12 -1.1788073734771771 1101 13 -1.3525400085955184 1101 14 -1.5125367056575882 1101 15 -1.7805739428761314 1101 16 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-0.08449656490915913 2315 9 -0.05976140150156084 2315 10 -0.22257035803473002 2315 11 -0.2754912473217459 2315 12 -0.23370410898684213 2315 13 -0.22984085564986856 2315 14 -0.39556706636949457 2315 15 -0.9415904080898017 2315 16 -0.6929438496182243 2315 17 -0.36612725009207026 2315 18 -0.19411837351419337 2315 19 -0.28362316923788455 2315 20 -0.25560809879240765 2315 21 -0.42419553362615403 2315 22 -0.09421615165095432 2315 23 -0.11497989280725562 2315 24 -0.2136412175017668 2315 25 0.1142041651525787 2316 1 1.297912977497429 2316 2 0.55977660857112 2316 3 0.15817765164836928 2316 4 -0.09802095967630464 2316 5 -0.06629776536416898 2316 6 -0.20434148962277732 2316 7 -0.07485391415760034 2316 8 -0.3356243844580362 2316 9 -0.3259268150354018 2316 10 -0.30978840314751466 2316 11 -0.227370946569863 2316 12 -0.22618531199435665 2316 13 -0.32006641955963744 2316 14 -0.41511593854994544 2316 15 -0.2528686035785199 2316 16 -0.3305378345806247 2316 17 -0.17514980648304856 2316 18 -0.23171235847659943 2316 19 -0.3933976053281114 2316 20 -0.258615617589399 2316 21 -0.17757899227277107 2316 22 -0.43256201631260893 2316 23 -0.34054380258169203 2316 24 -0.3625133979528954 2316 25 -0.351961248381258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8078.0 3 2.0 4 53.0 5 315.0 6 430.0 7 382.0 8 341.0 9 358.0 10 338.0 11 337.0 12 400.0 13 431.0 14 421.0 15 417.0 16 386.0 17 419.0 18 486.0 19 623.0 20 801.0 21 892.0 22 1107.0 23 1337.0 24 1608.0 25 1851.0 26 2209.0 27 2465.0 28 2995.0 29 3660.0 30 4666.0 31 6070.0 32 8341.0 33 11632.0 34 16781.0 35 25840.0 36 45443.0 37 83191.0 38 206799.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.73065184216264 17.463529176658675 11.506930818981179 17.298888162197514 2 18.951198309497276 20.4499079620227 38.837756679278854 21.761137049201164 3 22.347460686094898 30.516943515560975 26.377717693584234 20.757878104759893 4 12.954614584893925 17.405535958305528 35.007195747018415 34.63265370978213 5 13.221332255992518 39.06048920654303 29.379628725961265 18.338549811503185 6 28.331533526321046 34.081916930587944 19.874179877855113 17.712369665235897 7 27.36857406322254 34.855071476267696 18.400766774323962 19.375587686185796 8 29.757088696678696 27.510953710640422 21.296056664169445 21.43590092851143 9 31.797544522824683 11.652738984727575 19.234495574377906 37.31522091806983 10 23.092121231368086 25.296760978398204 24.43023933690679 27.180878453326923 11 35.68007695431518 24.805061924175497 18.785973079601888 20.72888804190743 12 26.702345112967883 26.293986465179486 26.47343740429094 20.53023101756169 13 35.007147557350635 20.413196886841686 25.314379070136823 19.26527648567085 14 25.69431367255121 24.860978448539907 27.399780832609206 22.044927046299676 15 29.682046204470563 24.526566548653676 27.119067462705548 18.67231978417021 16 22.71552870118095 27.204370223760364 24.56137560246234 25.518725472596344 17 21.979053498129453 25.946297314865742 24.82578674388265 27.248862443122157 18 22.381239351868633 20.41654583680719 31.830889549425457 25.37132526189872 19 22.19296655488081 24.753013686214086 32.901749297561864 20.152270461343242 20 26.870361930933157 17.840793921791356 32.66266259457523 22.626181552700256 21 27.967902191929294 21.3100331725722 31.68105354093967 19.041011094558836 22 26.69961768658458 21.828351021277655 30.618840096904098 20.853191195233663 23 26.458123474267875 21.592115651629413 31.85411051518699 20.09565035891572 24 24.96650300451017 21.500983335528616 30.615118386330504 22.91739527363071 25 23.62929591032291 23.291007526859733 32.589962218026756 20.48973434479061 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1002.0 1 1002.0 2 1185.5 3 1369.0 4 1369.0 5 1369.0 6 3143.5 7 4918.0 8 4918.0 9 4918.0 10 4358.5 11 3799.0 12 3799.0 13 3799.0 14 2316.0 15 833.0 16 833.0 17 833.0 18 1867.0 19 2901.0 20 2901.0 21 2901.0 22 4842.5 23 6784.0 24 6784.0 25 6784.0 26 13096.5 27 19409.0 28 19409.0 29 19409.0 30 22175.5 31 24942.0 32 24942.0 33 24942.0 34 25377.0 35 25812.0 36 25812.0 37 25812.0 38 21892.0 39 17972.0 40 17972.0 41 17972.0 42 25522.0 43 33072.0 44 33072.0 45 33072.0 46 53489.5 47 73907.0 48 73907.0 49 73907.0 50 72686.0 51 71465.0 52 71465.0 53 71465.0 54 61995.5 55 52526.0 56 52526.0 57 52526.0 58 44075.5 59 35625.0 60 35625.0 61 35625.0 62 34881.0 63 34137.0 64 34137.0 65 34137.0 66 25121.5 67 16106.0 68 16106.0 69 16106.0 70 12646.5 71 9187.0 72 9187.0 73 9187.0 74 6683.5 75 4180.0 76 4180.0 77 4180.0 78 2799.0 79 1418.0 80 1418.0 81 1418.0 82 899.5 83 381.0 84 381.0 85 381.0 86 238.5 87 96.0 88 96.0 89 96.0 90 71.5 91 47.0 92 47.0 93 47.0 94 30.0 95 13.0 96 13.0 97 13.0 98 8.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3514330003054955 2 0.30006449349973413 3 0.31952568991072744 4 0.3093425057421844 5 0.29734897772145596 6 0.2527692603613899 7 0.3663683370860253 8 0.32020456885529697 9 0.26612054627125736 10 0.24032314637761512 11 0.2543533112320522 12 0.2525429673798667 13 0.2704201129201978 14 0.25933175682556203 15 0.3525644652131114 16 0.415473914076555 17 0.43606657539516414 18 0.11608829952139035 19 0.13238139419105918 20 0.19325420622079406 21 0.19868523777735034 22 0.14573268010092666 23 0.25638994806576076 24 0.35460110204682 25 0.39556013170251525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 441905.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.62345979339451 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02357286932855 13.040811939217706 2 12.651583270553354 4.71232504723866 3 5.380446669420885 3.0060759665538974 4 2.93688789521009 2.1878005453660854 5 1.8979805098544313 1.7673481856960205 6 1.2965078130695764 1.448727667711386 7 0.831125908284527 1.0834907955329767 8 0.6427859729276532 0.9576718978060896 9 0.48968383192787185 0.8207646439845667 >10 2.966050207781477 10.668808906891753 >50 0.3256458237138205 4.199092565144092 >100 0.4362195922136625 18.07877258686822 >500 0.06075481785705607 8.01122413188355 >1k 0.055894432428491576 20.842488770210792 >5k 0.003645289071423364 5.707335286996074 >10k+ 0.0012150963571411213 3.467261062898134 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 15322 3.467261062898134 No Hit GGTATCAACGCAGAGTACTTTTTTT 9885 2.236906122356615 No Hit TATCAACGCAGAGTACTTTTTTTTT 8973 2.0305269232074767 No Hit GTACTGGTTCACTATCGGTCAGTCA 6363 1.439902241431982 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4511 1.0208076396510561 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4289 0.9705705977529107 No Hit GGATACCACGTGTCCCGCCCTACTC 3911 0.8850318507371493 No Hit ATCATTAACTGAATCCATAGGTTAA 3514 0.7951935370724478 No Hit GTACATGGGGGTTAAGCGACTAAGC 3462 0.7834263020332424 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3174 0.7182539233545672 No Hit GTATTTAGCCTTGGAGGATGGTCCC 2702 0.6114436360756271 No Hit ATACAGGGTGACAGCCCCGTACACA 2648 0.5992238150733755 No Hit GGTTAATGAGGCGAACCGGGGGAAC 2621 0.5931139045722498 No Hit GTATCAACGCAGAGTACATGGGGGT 2487 0.5627906450481438 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 2454 0.555322976657879 No Hit GTTCACTATCGGTCAGTCAGGAGTA 2261 0.5116484312239056 No Hit GGTATCAACGCAGAGTACATGGGGG 2236 0.5059911066858261 No Hit GTTAATGATAGTGTGTCGAAACACA 2132 0.4824566366074156 No Hit CTATCGGTCAGTCAGGAGTATTTAG 2116 0.4788359489030448 No Hit CCCATATTCAGACAGGATACCACGT 2109 0.47725189803238255 No Hit AGCGTACACGGTGGATGCCCTGGCA 2107 0.4767993120693361 No Hit TATTCAGACAGGATACCACGTGTCC 2073 0.4691053506975481 No Hit GCCTTGGAGGATGGTCCCCCCATAT 2058 0.4657109559747004 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 2001 0.4528122560278793 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1962 0.44398682974847536 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 1875 0.4242993403559589 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1770 0.40053857729602516 No Hit GTGGTATCAACGCAGAGTACATGGG 1749 0.3957864246840384 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 1730 0.39148685803509803 No Hit CGGTACTGGTTCACTATCGGTCAGT 1598 0.36161618447403854 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 1523 0.3446442108598002 No Hit GAGTACATGGGGGTTAAGCGACTAA 1511 0.34192869508152207 No Hit GGTTCACTATCGGTCAGTCAGGAGT 1508 0.34124981613695254 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 1497 0.3387605933401976 No Hit GTGCTAATCTGCGATAAGCGTCGGT 1480 0.3349136126543035 No Hit TATCAACGCAGAGTACATGGGGGTT 1420 0.32133603376291286 No Hit ACGCAGAGTACATGGGGGTTAAGCG 1412 0.31952568991072744 No Hit GTACTTAGATGTTTCAGTTCCCCCG 1398 0.3163575881694029 No Hit GTTATAACCGGCGATTTCCGAATGG 1342 0.303685181204105 No Hit TCACGGTACTGGTTCACTATCGGTC 1332 0.3014222513888732 No Hit GATATGAACCGTTATAACCGGCGAT 1315 0.2975752707029791 No Hit CATCTAAGTACCCCGAGGAAAAGAA 1313 0.29712268473993275 No Hit GGTGATATGAACCGTTATAACCGGC 1261 0.28535544970072757 No Hit GGAGTATTTAGCCTTGGAGGATGGT 1247 0.28218734795940303 No Hit TCATTAACCTATGGATTCAGTTAAT 1222 0.27653002342132355 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 1222 0.27653002342132355 No Hit ATCAACGCAGAGTACTTTTTTTTTT 1178 0.26657313223430373 No Hit GTGGTATCAACGCAGAGTACTTTTT 1148 0.2597843427886084 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 1137 0.25729511999185345 No Hit CACTATCATTAACTGAATCCATAGG 1088 0.24620676389721774 No Hit GGGTACTTAGATGTTTCAGTTCCCC 1000 0.22629298152317803 No Hit GTCCCGCCCTACTCATCGAGCTCAC 982 0.22221970785576087 No Hit CTATCATTAACTGAATCCATAGGTT 974 0.22040936400357541 No Hit TCGGTAAGGTGATATGAACCGTTAT 967 0.21882531313291317 No Hit CCGTTATAACCGGCGATTTCCGAAT 962 0.2176938482252973 No Hit ATCCATAGGTTAATGAGGCGAACCG 955 0.21610979735463506 No Hit GCGTACACGGTGGATGCCCTGGCAG 955 0.21610979735463506 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 940 0.21271540263178737 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 906 0.2050214412599993 No Hit CCTATGGATTCAGTTAATGATAGTG 900 0.20366368337086027 No Hit GCCCTACTCATCGAGCTCACAGCAT 882 0.19959040970344305 No Hit CACACACACTGATTCAGGCTCTGGG 855 0.19348049920231725 No Hit GGTATCAACGCAGAGTACATGGGGA 828 0.18737058870119142 No Hit GTACATGGGCATCTAAGTACCCCGA 805 0.18216585012615832 No Hit GTCCCCCCATATTCAGACAGGATAC 783 0.17718740453264842 No Hit AGCTCACAGCATGTGCATTTTTGTG 773 0.17492447471741662 No Hit TTTGTGTACGGGGCTGTCACCCTGT 714 0.16157318880754915 No Hit TAAGTACCCCGAGGAAAAGAAATCA 709 0.16044172389993325 No Hit GTGTGTTAGTGGAAGCGTCTGGAAA 709 0.16044172389993325 No Hit CCCTGGCAGTCAGAGGCGATGAAGG 701 0.15863138004774782 No Hit GTGTTTCGACACACTATCATTAACT 697 0.1577262081216551 No Hit AGTCAGGAGTATTTAGCCTTGGAGG 686 0.15523698532490016 No Hit CATTAACTGAATCCATAGGTTAATG 676 0.15297405550966836 No Hit GGGAAACCCAGTGTGTTTCGACACA 675 0.15274776252814518 No Hit GGGTTTCCCCATTCGGAAATCGCCG 673 0.15229517656509883 No Hit ATGATAGTGTGTCGAAACACACTGG 661 0.1495796607868207 No Hit GGGTGACAGCCCCGTACACAAAAAT 659 0.14912707482377432 No Hit TAATGATAGTGTGTCGAAACACACT 649 0.14686414500854256 No Hit ATTTAGCCTTGGAGGATGGTCCCCC 645 0.14595897308244984 No Hit GTGTGTGTTAGTGGAAGCGTCTGGA 634 0.1434697502856949 No Hit TTCCAGACGCTTCCACTAACACACA 622 0.14075423450741675 No Hit GTGGTATCCTGTCTGAATATGGGGG 615 0.1391701836367545 No Hit GTATCGCGCGCCTTTCCAGACGCTT 611 0.13826501171066177 No Hit GATTCAGGCTCTGGGCTGCTCCCCG 607 0.1373598397845691 No Hit GGGGTTAAGCGACTAAGCGTACACG 604 0.13668096083999953 No Hit GGTATCAACGCAGAGTACATGGGCA 601 0.13600208189543 No Hit GTGCATTTTTGTGTACGGGGCTGTC 600 0.13577578891390685 No Hit GTATCAACGCAGAGTACATGGGGAA 594 0.13441803102476776 No Hit GGAGGATGGTCCCCCCATATTCAGA 582 0.13170251524648963 No Hit CCACGTGTCCCGCCCTACTCATCGA 579 0.1310236363019201 No Hit GTACATGGGATAGGTTAATGAGGCG 576 0.13034475735735057 No Hit GGGGAAACCCAGTGTGTTTCGACAC 575 0.1301184643758274 No Hit CAGTAGCGGCGAGCGAACGGGGAGC 574 0.1298921713943042 No Hit AAGTACCCCGAGGAAAAGAAATCAA 569 0.12876070648668833 No Hit TCCCCCAGTAGCGGCGAGCGAACGG 530 0.11993528020728438 No Hit CCCTCACGGTACTGGTTCACTATCG 529 0.1197089872257612 No Hit AGGTTAATGAGGCGAACCGGGGGAA 529 0.1197089872257612 No Hit GTCAGAGGCGATGAAGGACGTGCTA 520 0.1176723503920526 No Hit GTATCAACGCAGAGTACATGGGGAG 516 0.11676717846595987 No Hit GTATCAACGCAGAGTACATGGGCAT 514 0.11631459250291352 No Hit GAATATGGGGGGACCATCCTCCAAG 495 0.11201502585397313 No Hit CATTAACCTATGGATTCAGTTAATG 486 0.10997838902026454 No Hit CGGTGGATGCCCTGGCAGTCAGAGG 486 0.10997838902026454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 484 0.10952580305721817 No Hit GTACGGGGCTGTCACCCTGTATCGC 481 0.10884692411264865 No Hit CACTGATTCAGGCTCTGGGCTGCTC 477 0.10794175218655593 No Hit GGTATCAACGCAGAGTACATGGGAT 471 0.10658399429741687 No Hit GGGCTGTCACCCTGTATCGCGCGCC 464 0.10499994342675462 No Hit ATTCAGACAGGATACCACGTGTCCC 461 0.10432106448218507 No Hit GACTAAGCGTACACGGTGGATGCCC 461 0.10432106448218507 No Hit GAGTAGGGCGGGACACGTGGTATCC 459 0.10386847851913875 No Hit CAACGCAGAGTACTTTTTTTTTTTT 456 0.10318959957456919 No Hit CAGTTAATGATAGTGTGTCGAAACA 449 0.10160554870390695 No Hit ACCCAGTGTGTTTCGACACACTATC 446 0.10092666975933741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTTGC 25 0.005987753 19.0206 15 CTACATG 35 9.888551E-5 19.007618 1 TTAGGAC 25 0.0060153636 19.005457 3 ACACCGT 30 7.7017077E-4 19.003298 6 TAGGACC 25 0.0060193166 19.003298 4 GGACGTT 30 7.7786145E-4 18.97525 10 ATATCAA 85 3.6379788E-12 17.889524 1 ATACATG 45 3.5174642E-5 16.895662 1 GAGGTTA 40 2.795095E-4 16.593924 9 GGAGGTT 40 2.8023627E-4 16.588276 8 ATTTGCC 35 0.0021512427 16.30337 16 TCGTAAG 35 0.0021610772 16.292244 13 CTTAGGA 35 0.0021627198 16.290392 2 TAGACCC 35 0.0021643636 16.28854 4 GACCCGA 35 0.0021643636 16.28854 6 ACGTTAG 35 0.0021775519 16.273737 12 GGTATCA 2135 0.0 15.00133 1 CGTATCA 45 6.7282084E-4 14.783704 1 CGTAAGG 40 0.0052333768 14.265449 14 AGTATCA 40 0.005257138 14.255714 1 >>END_MODULE