FastQCFastQC Report
Thu 26 May 2016
SRR1512287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441905
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT168953.823219922834093No Hit
GGTATCAACGCAGAGTACTTTTTTT111252.517509419445356No Hit
TATCAACGCAGAGTACTTTTTTTTT107112.42382412509476No Hit
GTACTGGTTCACTATCGGTCAGTCA63621.4396759484504589No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54901.2423484685622477No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44921.0165080730021159No Hit
GGATACCACGTGTCCCGCCCTACTC41390.936626650524434No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37300.8440728210814541No Hit
ATCATTAACTGAATCCATAGGTTAA37200.8418098912662224No Hit
GTACATGGGGGTTAAGCGACTAAGC35200.7965512949615868No Hit
ATACAGGGTGACAGCCCCGTACACA29400.6653013656781435No Hit
GGTTAATGAGGCGAACCGGGGGAAC27910.63158371143119No Hit
GTATTTAGCCTTGGAGGATGGTCCC26900.6087281202973489No Hit
GTGGATGCCCTGGCAGTCAGAGGCG26190.5926613186092033No Hit
GTATCAACGCAGAGTACATGGGGGT25020.5661850397709914No Hit
AGCGTACACGGTGGATGCCCTGGCA23310.527488939930528No Hit
CTATCGGTCAGTCAGGAGTATTTAG23140.523641959244634No Hit
CCCATATTCAGACAGGATACCACGT23000.5204738575033095No Hit
GTTCACTATCGGTCAGTCAGGAGTA22510.5093855014086738No Hit
GGTATCAACGCAGAGTACATGGGGG22510.5093855014086738No Hit
TATTCAGACAGGATACCACGTGTCC22420.5073488645749652No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22290.5044070558151639No Hit
ACTTAGATGTTTCAGTTCCCCCGGT21730.49173464884986595No Hit
GTTAATGATAGTGTGTCGAAACACA21670.49037689096072684No Hit
GTCAGGAGTATTTAGCCTTGGAGGA20500.463900612122515No Hit
GCCTTGGAGGATGGTCCCCCCATAT19550.4424027788778131No Hit
GTGGTATCAACGCAGAGTACATGGG18990.4297303719125152No Hit
TCTAAGTACCCCGAGGAAAAGAAAT18860.4267885631527138No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18480.41818942985483304No Hit
CGGTACTGGTTCACTATCGGTCAGT17640.3991808194068861No Hit
GCCCAGAGCCTGAATCAGTGTGTGT16000.3620687704370849No Hit
GGTTCACTATCGGTCAGTCAGGAGT15570.35233817223158825No Hit
ATCAACGCAGAGTACTTTTTTTTTT15560.3521118792500651No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG15260.3453230898043697No Hit
TCACGGTACTGGTTCACTATCGGTC15050.34057093719238296No Hit
GTGCTAATCTGCGATAAGCGTCGGT14950.3383080073771512No Hit
GAGTACATGGGGGTTAAGCGACTAA14890.3369502494880121No Hit
ACGCAGAGTACATGGGGGTTAAGCG14620.3308403389868863No Hit
TATCAACGCAGAGTACATGGGGGTT14600.33038775302384No Hit
GTACTTAGATGTTTCAGTTCCCCCG14560.32948258109774725No Hit
GTGGTATCAACGCAGAGTACTTTTT14370.3251830144488069No Hit
GCCTGAATCAGTGTGTGTGTTAGTG13880.31409465835417116No Hit
GTTATAACCGGCGATTTCCGAATGG13720.3104739706498003No Hit
CATCTAAGTACCCCGAGGAAAAGAA13470.3048166461117208No Hit
GATATGAACCGTTATAACCGGCGAT13380.30278000927801224No Hit
TCATTAACCTATGGATTCAGTTAAT13180.29825414964754865No Hit
GGTGATATGAACCGTTATAACCGGC12680.2869395005713898No Hit
GGAGTATTTAGCCTTGGAGGATGGT12290.27811407429198587No Hit
GAATCAGTGTGTGTGTTAGTGGAAG11970.27087269888324417No Hit
CACTATCATTAACTGAATCCATAGG11560.2615946866407938No Hit
ATCCATAGGTTAATGAGGCGAACCG10870.24598047091569453No Hit
GCGTACACGGTGGATGCCCTGGCAG10730.24281236917437005No Hit
GGGTACTTAGATGTTTCAGTTCCCC10190.23059254817211847No Hit
CCTATGGATTCAGTTAATGATAGTG10050.22742444643079396No Hit
CTATCATTAACTGAATCCATAGGTT10010.22651927450470125No Hit
TCGGTAAGGTGATATGAACCGTTAT9820.22221970785576087No Hit
CCCCAGTAGCGGCGAGCGAACGGGG9730.22018307102205226No Hit
CACACACACTGATTCAGGCTCTGGG9610.2174675552437741No Hit
GTCCCGCCCTACTCATCGAGCTCAC9590.21701496928072775No Hit
CCGTTATAACCGGCGATTTCCGAAT9510.21520462542854232No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG9190.20796325001980065No Hit
GCCCTACTCATCGAGCTCACAGCAT9130.20660549213066154No Hit
GGTATCAACGCAGAGTACATGGGGA8780.19868523777735034No Hit
TTTGTGTACGGGGCTGTCACCCTGT7910.17899774838483384No Hit
AGCTCACAGCATGTGCATTTTTGTG7800.1765085255880789No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA7740.1751507676989398No Hit
CATTAACTGAATCCATAGGTTAATG7650.1731141308652312No Hit
TAAGTACCCCGAGGAAAAGAAATCA7630.17266154490218485No Hit
GTCCCCCCATATTCAGACAGGATAC7600.17198266595761533No Hit
CAGTAGCGGCGAGCGAACGGGGAGC7530.1703986150869531No Hit
ATGATAGTGTGTCGAAACACACTGG7480.1692671501793372No Hit
AGTCAGGAGTATTTAGCCTTGGAGG7470.169040857197814No Hit
GGGAAACCCAGTGTGTTTCGACACA7250.1640624116043041No Hit
GTACATGGGCATCTAAGTACCCCGA7210.16315723967821139No Hit
TAATGATAGTGTGTCGAAACACACT7160.1620257747705955No Hit
GGGTGACAGCCCCGTACACAAAAAT7090.16044172389993325No Hit
CCCTGGCAGTCAGAGGCGATGAAGG7070.15998913793688688No Hit
GTGTTTCGACACACTATCATTAACT6890.15591586426946968No Hit
GGGTTTCCCCATTCGGAAATCGCCG6860.15523698532490016No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA6550.14822190289768164No Hit
TCCCCCAGTAGCGGCGAGCGAACGG6480.14663785202701937No Hit
GTGGTATCCTGTCTGAATATGGGGG6480.14663785202701937No Hit
TTCCAGACGCTTCCACTAACACACA6440.14573268010092666No Hit
AGGTTAATGAGGCGAACCGGGGGAA6440.14573268010092666No Hit
ATTTAGCCTTGGAGGATGGTCCCCC6420.14528009413788032No Hit
GGTATCAACGCAGAGTACATGGGCA6350.14369604326721805No Hit
GGAGGATGGTCCCCCCATATTCAGA6290.142338285378079No Hit
GGGGAAACCCAGTGTGTTTCGACAC6000.13577578891390685No Hit
CCACGTGTCCCGCCCTACTCATCGA5980.13532320295086048No Hit
GGGGTTAAGCGACTAAGCGTACACG5950.13464432400629092No Hit
AAGTACCCCGAGGAAAAGAAATCAA5900.13351285909867505No Hit
GTACATGGGATAGGTTAATGAGGCG5900.13351285909867505No Hit
CATTAACCTATGGATTCAGTTAATG5800.13124992928344326No Hit
GATTCAGGCTCTGGGCTGCTCCCCG5690.12876070648668833No Hit
GTATCGCGCGCCTTTCCAGACGCTT5670.12830812052364196No Hit
CGGTGGATGCCCTGGCAGTCAGAGG5570.12604519070841017No Hit
GTCAGAGGCGATGAAGGACGTGCTA5550.12559260474536382No Hit
GTGCATTTTTGTGTACGGGGCTGTC5500.12446113983774792No Hit
GGGCTGTCACCCTGTATCGCGCGCC5230.11835122933662214No Hit
GGTATCAACGCAGAGTACATGGGAT5200.1176723503920526No Hit
GTATCAACGCAGAGTACATGGGGAA5190.11744605741052941No Hit
CAGTTAATGATAGTGTGTCGAAACA5130.11608829952139035No Hit
CAACGCAGAGTACTTTTTTTTTTTT5050.11427795566920493No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT5040.11405166268768174No Hit
ATTCAGACAGGATACCACGTGTCCC4960.11224131883549632No Hit
AACACACACACTGATTCAGGCTCTG4940.11178873287244996No Hit
GTACGGGGCTGTCACCCTGTATCGC4920.11133614690940359No Hit
GTATCAACGCAGAGTACATGGGGAG4900.11088356094635726No Hit
GAATATGGGGGGACCATCCTCCAAG4860.10997838902026454No Hit
CCCTCACGGTACTGGTTCACTATCG4860.10997838902026454No Hit
GTATCAACGCAGAGTACATGGGCAT4860.10997838902026454No Hit
GAGTAGGGCGGGACACGTGGTATCC4820.10907321709417184No Hit
TTTCAGTTCCCCCGGTTCGCCTCAT4760.10771545920503275No Hit
GACTAAGCGTACACGGTGGATGCCC4760.10771545920503275No Hit
GCATGTGCATTTTTGTGTACGGGGC4620.10454735746370826No Hit
GCGATAAGCGTCGGTAAGGTGATAT4570.10341589255609238No Hit
ACATGGGGGTTAAGCGACTAAGCGT4540.10273701361152283No Hit
CGTGGTATCCTGTCTGAATATGGGG4440.10047408379629105No Hit
TATTTAGCCTTGGAGGATGGTCCCC4430.1002477908147679No Hit
ATGAAGGACGTGCTAATCTGCGATA4420.10002149783324471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC504.5455963E-617.1007144
TTAGGAC453.528378E-516.8895933
CTTAGGA453.528378E-516.8895932
GGTATCA24600.015.3932151
GTATAGA400.00521847514.2715981
GAGGTTA400.005261819314.2538219
AAATCGC954.9003575E-913.99266417
AATCGCC954.9003575E-913.99266418
GACCGTT551.9488399E-413.8234537
AGGACCG551.9544657E-413.8187585
AGGGTGA4800.013.4588945
GAAATCG1001.0071744E-813.29303116
ATCGCCG1051.9934305E-812.66002819
ACAGGGT5100.012.4809123
CCGAATG2700.012.30836118
CAGGGTG5450.012.0280254
CGAATGG2800.011.86877719
TACAGGG5450.011.6793862
CGGAAAT1157.0729584E-811.55915614
GGGAGCC1001.9103245E-611.4043497
Produced by FastQC (version 0.11.5)