FastQCFastQC Report
Thu 26 May 2016
SRR1512286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285049
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55730.24388973715662116No Hit
GTCCTACAGTGGACATTTCTAAATT54380.2379817675682228No Hit
GTCCTAAAGTGTGTATTTCTCATTT49830.21806972191843588No Hit
CTGTAGGACGTGGAATATGGCAAGA46640.20410940859473908No Hit
CTTTAGGACGTGAAATATGGCGAGG41620.18214051427343572No Hit
GTCCTACAGTGTGCATTTCTCATTT34630.15155036062683994No Hit
GGTATCAACGCAGAGTACTTTTTTT34090.1491871727914806No Hit
TATCAACGCAGAGTACTTTTTTTTT30430.1331700107962674No Hit
CTGTAGGACCTGGAATATGGCGAGA25030.1095381324426741No Hit
CTGAAGGACCTGGAATATGGCGAGA23210.10157331418275933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10350.013.8611714
GGTATCA12250.013.5729691
GCGACTA1850.011.82281316
AGGACCT20450.011.5680415
GGACCTG20200.011.5228246
CGACTAA1900.011.5119417
TAAGCGA1950.011.206413
GTCCTAC28700.011.1232141
GGCGAGG11950.011.05535319
TGTAGGA32300.011.030422
AAGCGAC2000.010.9353814
TCCTACA31600.010.7937092
GTAGGAC31650.010.7766563
AGTCGTC2650.010.734458
GACTGCG803.770469E-410.6880067
GTCTTAG2500.010.6412071
CTGTAGG30400.010.5949521
CCTACAG31500.010.4358753
CATTTCT37900.010.38716814
GTCCTAA14200.010.3039871
Produced by FastQC (version 0.11.5)