FastQCFastQC Report
Thu 26 May 2016
SRR1512286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285049
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64280.2813068778831439No Hit
GTCCTACAGTGGACATTTCTAAATT56100.24550895845121923No Hit
GTCCTAAAGTGTGTATTTCTCATTT51330.2246341325722118No Hit
CTGTAGGACGTGGAATATGGCAAGA51000.2231899622283811No Hit
CTTTAGGACGTGAAATATGGCGAGG43140.18879245040259532No Hit
GGTATCAACGCAGAGTACTTTTTTT42080.18415360020726032No Hit
TATCAACGCAGAGTACTTTTTTTTT39630.17343172947275967No Hit
GTCCTACAGTGTGCATTTCTCATTT35540.15553276975679733No Hit
CTGTAGGACCTGGAATATGGCGAGA26260.11492094917877034No Hit
CTGAAGGACCTGGAATATGGCGAGA23470.10271114536274714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAT250.006039353618.99649611
CTAACGT508.693086E-515.2048554
GGTATCA13650.013.4471281
TAGGACC9600.012.8686924
TATACCG550.00306086912.09535
TAGAAAT7950.011.2362924
GGCGAGG12600.011.07765319
GTGGTAT4600.010.7510381
AGGACCT18950.010.7321285
GTCCTAA14950.010.6874231
TGTAGGA30650.010.4794632
TTTAGAA9150.010.3856022
GTAGGAC30650.010.2316513
GCCGGTT1303.853038E-710.22888311
TAGTACG750.002641380510.1365694
AGGACGT29400.010.1176195
TTAGGAC17950.010.1118623
GGACGTG29250.010.1054096
CTGTAGG30550.010.0864581
GATATAC5000.010.0811651
Produced by FastQC (version 0.11.5)