FastQCFastQC Report
Thu 26 May 2016
SRR1512285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725303
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT108530.39823094899906547No Hit
CTGTAGGACGTGGAATATGGCAAGA97480.35768499869555787No Hit
GTCCTAAAGTGTGTATTTCTCATTT91160.3344949167120133No Hit
CTTTAGGACGTGAAATATGGCGAGG78700.28877522976344283No Hit
GTATCAACGCAGAGTACTTTTTTTT66330.24338578132413166No Hit
GTCCTACAGTGTGCATTTCTCATTT52610.19304275524593045No Hit
CTGAAGGACCTGGAATATGGCGAGA43250.15869795028295935No Hit
GGTATCAACGCAGAGTACTTTTTTT39460.1447912397263717No Hit
CTGTAGGACCTGGAATATGGCGAGA38030.1395441167459178No Hit
TATCAACGCAGAGTACTTTTTTTTT36550.13411352792698647No Hit
GTCCTTCAGTGTGCATTTCTCATTT35820.13143492668521628No Hit
ATTTAGAAATGTCCACTGTAGGACG34080.12505031550620244No Hit
TTTCTAAATTTTCCACCTTTTTCAG30990.11371212668829851No Hit
GAATATGGCAAGAAAACTGAAAATC29580.10853839004323555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTCG602.5505988E-514.26125214
TAGGACC14200.013.3152934
GGTATCA14050.013.1876771
TCGAACT1457.2759576E-1212.45488719
TACGATA802.8639917E-511.8767062
CGATACT658.017136E-411.6939874
TCCAACG2450.011.63389518
TAGAAAT13800.011.4980294
ACGATAC752.0721352E-411.4016383
GGCGAGG19800.011.23323519
GTCCTAC48850.011.106611
AATGTCC13350.011.0810588
TCCTACA52600.011.03675652
AAATGTC13000.010.9631127
GTCGAGA700.001485428310.86290919
TGTAGGA53650.010.7499132
CCTACAG52700.010.7093183
CACCTTT11400.010.5916914
GAAATGT13750.010.5031336
AGGACCT31000.010.4819575
Produced by FastQC (version 0.11.5)