FastQCFastQC Report
Thu 26 May 2016
SRR1512285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725303
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT111930.41070662601552926No Hit
CTGTAGGACGTGGAATATGGCAAGA104820.3846177837840416No Hit
GTCCTAAAGTGTGTATTTCTCATTT93570.3433379701266245No Hit
CTTTAGGACGTGAAATATGGCGAGG84220.3090298583313489No Hit
GTATCAACGCAGAGTACTTTTTTTT77090.2828676297644702No Hit
GTCCTACAGTGTGCATTTCTCATTT55080.20210596766671451No Hit
CTGAAGGACCTGGAATATGGCGAGA47390.1738889217088889No Hit
GGTATCAACGCAGAGTACTTTTTTT47130.17293489934880635No Hit
TATCAACGCAGAGTACTTTTTTTTT45540.1671006856852247No Hit
CTGTAGGACCTGGAATATGGCGAGA40990.15040529438378045No Hit
ATTTAGAAATGTCCACTGTAGGACG36580.13422360743007292No Hit
GTCCTTCAGTGTGCATTTCTCATTT35970.1319853242006485No Hit
TTTCTAAATTTTCCACCTTTTTCAG34650.12714182606484492No Hit
GAATATGGCAAGAAAACTGAAAATC31290.11481292171916296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.014.6167791
ACCGCGC500.001494142813.306978
CACGCGG500.001500774113.29915110
TAGGACC15650.012.6895854
CGTGCGC1301.9826984E-1012.42228410
ACCGGGC550.003057446612.0972458
TATAGCG658.065817E-411.68555516
ACCTTTT12450.011.515223515
GGCGAGG21750.011.48051219
GACCGCT752.0648362E-411.4059747
CACCTTT12500.011.24171114
TAGAAAT13400.011.0620424
GTCCTAC48450.010.9930121
TTATCCC1658.0035534E-1110.94170954
TGTAGGA53350.010.775082
GAAATGT12200.010.7511386
AAATGTC12250.010.7076497
AGTACCG803.7639876E-410.6901565
TCCAACG2150.010.59833218
TCCTACA51100.010.5615352
Produced by FastQC (version 0.11.5)