FastQCFastQC Report
Thu 26 May 2016
SRR1512284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2467196
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78870.31967464279287094No Hit
CTGTAGGACGTGGAATATGGCAAGA69820.28299332521615633No Hit
GTCCTAAAGTGTGTATTTCTCATTT54760.2219523702210931No Hit
GTATCAACGCAGAGTACTTTTTTTT51050.2069150566067714No Hit
GTCCTACAGTGTGCATTTCTCATTT47270.19159402009406631No Hit
CTTTAGGACGTGAAATATGGCGAGG44170.17902914887994306No Hit
CTGTAGGACCTGGAATATGGCGAGA34330.13914581573575832No Hit
GGTATCAACGCAGAGTACTTTTTTT31740.1286480684955715No Hit
TATCAACGCAGAGTACTTTTTTTTT28240.11446192357640009No Hit
CTGAAGGACCTGGAATATGGCGAGA26330.10672034163479512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14000.013.6388184
CCGCGCG500.001516326613.2808829
CCAACGA2100.013.12822219
GGTATCA10950.013.0135161
TATTGCG701.0810018E-412.22681416
AAGCGTG550.00306963812.090747
ACCGTCT951.062459E-611.9793418
TGTAGGA40200.011.911012
TCCAACG3550.011.77760118
AGGACCT24500.011.7098025
AACCGTC658.027267E-411.6921447
TTAGACT1800.011.6107134
GTAGGAC39250.011.4247423
GGACCTG23450.011.38270956
CTGTAGG37750.011.349511
GTCCTAC37450.011.1106591
GTGTAGG6000.011.0831791
TTAGACG600.00587880311.0829544
GTGCGAT600.00590469311.07641211
GGCGAGG13600.011.04448319