FastQCFastQC Report
Thu 26 May 2016
SRR1512284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2467196
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79360.3216607030815549No Hit
CTGTAGGACGTGGAATATGGCAAGA74300.3011515907126957No Hit
GTATCAACGCAGAGTACTTTTTTTT62140.25186487007923164No Hit
GTCCTAAAGTGTGTATTTCTCATTT57270.2321258627202703No Hit
CTTTAGGACGTGAAATATGGCGAGG49600.2010379394259718No Hit
GTCCTACAGTGTGCATTTCTCATTT47990.19451231276315298No Hit
GGTATCAACGCAGAGTACTTTTTTT40190.16289747551471387No Hit
TATCAACGCAGAGTACTTTTTTTTT37770.1530887695991725No Hit
CTGTAGGACCTGGAATATGGCGAGA37380.15150802773675054No Hit
CTGAAGGACCTGGAATATGGCGAGA29430.11928521284891835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2450.013.95008618
TAGGACC12550.013.780764
GGTATCA13750.013.2094491
CCAACGA1900.012.49185319
GCGCCAC1251.4169927E-912.157248513
TAGTACG550.00306287612.0943054
GGCGAAA658.07037E-411.68468819
AGGACCT21900.011.4993615
GGACCTG21650.011.4588946
TGTAGGA40350.011.4453592
GTAGGAC39150.011.2864653
GTCTTAC2300.011.1632321
GGACGTG34100.011.0940156
GTCCTAC37950.011.02541351
CTGTAGG40250.011.0096631
TTAGGAC18300.011.0083313
AGGACGT34450.011.0066625
TAGGACA10600.010.9370334
CCGTTTC2000.010.92252813
GGCGAGG15050.010.91314919
Produced by FastQC (version 0.11.5)