FastQCFastQC Report
Thu 26 May 2016
SRR1512283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656503
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58990.3561116400030667No Hit
GTCCTACAGTGGACATTTCTAAATT54930.3316021763920741No Hit
CTGTAGGACGTGGAATATGGCAAGA54080.3264708847493787No Hit
GTCCTAAAGTGTGTATTTCTCATTT53560.32333174162678846No Hit
CTTTAGGACGTGAAATATGGCGAGG46660.2816777271154957No Hit
GGTATCAACGCAGAGTACTTTTTTT36920.22287916170390273No Hit
TATCAACGCAGAGTACTTTTTTTTT33590.20277657209193103No Hit
CTGAAGGACCTGGAATATGGCGAGA25900.1563534747597801No Hit
GTCCTTCAGTGTGCATTTCTCATTT24250.14639273215925355No Hit
GTCCTACAGTGTGCATTTCTCATTT22760.13739787975029322No Hit
ATTTAGAAATGTCCACTGTAGGACG21460.1295500219438178No Hit
TTTCTAAATTTTCCACCTTTTTCAG19250.11620866367280952No Hit
GAATATGGCAAGAAAACTGAAAATC18860.11385430633086689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7400.013.8680544
GGTATCA9200.013.73352051
TACACCG500.00149701713.303065
CCGCTCT550.003113086612.0676839
AGGACCT16150.011.5909015
GTATAAG1001.9299168E-611.3998551
GGCGAGG14250.011.2080219
ATACGGC700.001478102510.86887815
CTAAGAC1408.3182385E-910.859643
TTTATAC2100.010.859643
CCACCTT7500.010.76685513
ATTAGAC803.762927E-410.6899593
GGACCTG16900.010.6826066
ACCGTGC803.8460564E-410.6647098
AATGTCC6250.010.617318
TGTAGGA30500.010.5614462
TAGGACT3600.010.5579834
GTAATAC1451.4377292E-810.485173
ACCTTTT8200.010.438115
CACCTTT8150.010.385447514
Produced by FastQC (version 0.11.5)