FastQCFastQC Report
Thu 26 May 2016
SRR1512283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656503
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67270.40609645741661804No Hit
CTGTAGGACGTGGAATATGGCAAGA57920.3496522493469677No Hit
GTCCTACAGTGGACATTTCTAAATT56180.3391481935136852No Hit
GTCCTAAAGTGTGTATTTCTCATTT54480.32888561022829416No Hit
CTTTAGGACGTGAAATATGGCGAGG50070.30226326182325053No Hit
GGTATCAACGCAGAGTACTTTTTTT44050.26592164336557195No Hit
TATCAACGCAGAGTACTTTTTTTTT42670.2575908404633134No Hit
CTGAAGGACCTGGAATATGGCGAGA27620.16673679431911684No Hit
GTCCTACAGTGTGCATTTCTCATTT24020.14500426500887714No Hit
ATTTAGAAATGTCCACTGTAGGACG23160.13981260522920874No Hit
GTCCTTCAGTGTGCATTTCTCATTT22890.1381826655309408No Hit
TTTCTAAATTTTCCACCTTTTTCAG21840.13184401114878752No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20810.12562609304058006No Hit
GAATATGGCAAGAAAACTGAAAATC18870.11391467446783979No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17670.10667049803109321No Hit
GGAATATGGCGAGAAAACTGAAAAT17270.10425577255217769No Hit
ATTCCAGGTCCTTCAGTGTGCATTT16740.10105626129261462No Hit
CTGTAGGACCTGGAATATGGCGAGA16690.10075442060775018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.013.3266771
TCTAGAC801.989829E-613.0682143
TAGGACC6800.012.7187974
TAGAAAT7600.012.5054684
GGCGAGG11850.012.09725319
CCCGTAC658.071875E-411.68400316
GAAATGT7950.011.5980196
AAATGTC7700.011.4807817
GTCTAAC752.0476549E-411.4156831
CCCCGTA752.0871166E-411.39224715
TTAGAAA8200.011.0109123
ATTTAGA8400.010.985331
AATCCCG1303.2985554E-810.95408319
AATGTCC8350.010.9285858
AGGACCT17850.010.9154675
GTATCAA24400.010.8776481
CCCTTAT700.00147448210.8720791
TTTAGAA8900.010.7856152
TAGGACA6750.010.7009754
TTATACC803.7557428E-410.6921764
Produced by FastQC (version 0.11.5)