FastQCFastQC Report
Thu 26 May 2016
SRR1512282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817579
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78380.43123297529295834No Hit
CTGTAGGACGTGGAATATGGCAAGA73640.4051543289177527No Hit
GTCCTAAAGTGTGTATTTCTCATTT73060.40196327092247436No Hit
GTATCAACGCAGAGTACTTTTTTTT73060.40196327092247436No Hit
CTTTAGGACGTGAAATATGGCGAGG60540.33308043281750066No Hit
GGTATCAACGCAGAGTACTTTTTTT45880.25242369107477586No Hit
TATCAACGCAGAGTACTTTTTTTTT42230.2323420330010415No Hit
GTCCTACAGTGTGCATTTCTCATTT37910.2085741527603477No Hit
CTGAAGGACCTGGAATATGGCGAGA34310.18876758589310286No Hit
GTCCTTCAGTGTGCATTTCTCATTT30460.16758556299341046No Hit
ATTTAGAAATGTCCACTGTAGGACG29440.16197370238102443No Hit
TTTCTAAATTTTCCACCTTTTTCAG25690.14134186189431105No Hit
CTGTAGGACCTGGAATATGGCGAGA24370.13407945404298793No Hit
GAATATGGCAAGAAAACTGAAAATC23680.1302831953934327No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21430.11790409110140466No Hit
GGAATATGGCGAGAAAACTGAAAAT21100.11608848913857389No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20530.11295244938459346No Hit
ATTCCAGGTCCTTCAGTGTGCATTT18930.1041495307769291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA551.9460342E-413.830193516
CATTTCG551.946491E-413.82981214
GGTATCA12200.013.7825661
ATTTCGC500.001490235613.31119415
TAGGACC10300.012.729364
GTCCTAG2150.012.3718951
AATGTCC9050.012.2602218
ACACCGT951.0392341E-612.0008036
AAATGTC9600.011.4799367
TAGGACT3500.011.4010784
GTGGTAT3250.011.1075141
TAGAAAT10300.011.0690084
TGTCGAG951.35733535E-511.00225718
GAAATGT10300.010.9764626
TAAGCGA1658.0035534E-1110.94103313
ATGTCCA10350.010.9080579
GGGCCGT700.001490774610.8578696
GTATTAG1408.352799E-910.8569691
GGCGAGG17800.010.841289519
GTCCTAA17650.010.7108761
Produced by FastQC (version 0.11.5)