##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512282_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817579 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.05830723176269 31.0 30.0 34.0 16.0 34.0 2 29.93638130722241 31.0 30.0 34.0 23.0 34.0 3 30.59937807380037 33.0 31.0 34.0 26.0 34.0 4 34.2495886011007 37.0 35.0 37.0 31.0 37.0 5 34.42240034683499 37.0 35.0 37.0 32.0 37.0 6 34.57753913309958 37.0 35.0 37.0 32.0 37.0 7 34.519641787234555 37.0 35.0 37.0 32.0 37.0 8 34.51256314030917 37.0 35.0 37.0 32.0 37.0 9 36.10385738391564 39.0 37.0 39.0 32.0 39.0 10 36.02734461610747 39.0 37.0 39.0 32.0 39.0 11 36.04983882406212 39.0 37.0 39.0 32.0 39.0 12 35.996762176499615 39.0 37.0 39.0 32.0 39.0 13 35.96840357420503 39.0 37.0 39.0 32.0 39.0 14 37.199311831837846 40.0 38.0 41.0 32.0 41.0 15 37.13873894889851 40.0 38.0 41.0 31.0 41.0 16 37.07215477291496 40.0 38.0 41.0 31.0 41.0 17 37.04420385578839 40.0 38.0 41.0 31.0 41.0 18 36.831135813078824 40.0 38.0 41.0 30.0 41.0 19 36.936225055417125 40.0 38.0 41.0 31.0 41.0 20 36.87908311000512 40.0 38.0 41.0 31.0 41.0 21 36.82063613190954 40.0 38.0 41.0 31.0 41.0 22 36.7499195358221 40.0 38.0 41.0 31.0 41.0 23 36.62316521042552 40.0 38.0 41.0 30.0 41.0 24 36.52009788845492 40.0 37.0 41.0 30.0 41.0 25 36.40052619445977 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0456380774120468 1101 2 0.1392494158572113 1101 3 -0.43657334481247645 1101 4 -0.4421344851386806 1101 5 -0.7648443653059402 1101 6 -0.7453182376055807 1101 7 -0.8997833887517075 1101 8 -1.123661843421182 1101 9 -0.9062352059936174 1101 10 -1.0146548622485696 1101 11 -0.9820852288360982 1101 12 -1.1848519217540598 1101 13 -1.1323474541428666 1101 14 -1.3236478924236366 1101 15 -1.6518419671944784 1101 16 -1.5690662148593688 1101 17 -1.7920561771306183 1101 18 -1.6843664878696956 1101 19 -1.6682929113272493 1101 20 -1.8524088127909337 1101 21 -1.935259384050859 1101 22 -1.7843241236491991 1101 23 -1.9581392670876738 1101 24 -2.2850633413896873 1101 25 -2.1817327085666136 1102 1 1.0703352047919985 1102 2 0.35738074516113727 1102 3 -0.19029554257362236 1102 4 -0.20468301814112522 1102 5 -0.3178517436565471 1102 6 -0.32585166700423684 1102 7 -0.37326402224915256 1102 8 -0.49610310347802056 1102 9 -0.7137049520876246 1102 10 -0.5920291434264371 1102 11 -0.7894602438463068 1102 12 -1.0514778940819127 1102 13 -1.0022086264066772 1102 14 -1.2928339389352672 1102 15 -1.2759533629435822 1102 16 -1.1662569712403723 1102 17 -1.2757697690397833 1102 18 -1.178502500358185 1102 19 -1.1678789631758377 1102 20 -1.42735611028656 1102 21 -1.4342253475727205 1102 22 -1.3798367388942339 1102 23 -1.6251598410779877 1102 24 -1.5037757324822962 1102 25 -1.5373108350259415 1103 1 1.0651580176380655 1103 2 0.28476169754209124 1103 3 -0.2090386212004205 1103 4 -0.2730661853615004 1103 5 -0.39321165506297007 1103 6 -0.4766822317882955 1103 7 -0.6630757608980957 1103 8 -0.5407321178744766 1103 9 -0.7133173551884013 1103 10 -0.7499748798605452 1103 11 -0.8396817277887152 1103 12 -0.9448887467950939 1103 13 -0.9855142742472083 1103 14 -1.145907028636259 1103 15 -1.111307737251444 1103 16 -1.239402043222654 1103 17 -1.2680732020408954 1103 18 -1.18736185805475 1103 19 -1.4637538247483732 1103 20 -1.3054568854803605 1103 21 -1.574867651005718 1103 22 -1.707051578318378 1103 23 -1.7534267292285932 1103 24 -1.6887978808765425 1103 25 -1.5405500376837509 1104 1 1.3374205189382131 1104 2 0.546925265269067 1104 3 0.10971409381337693 1104 4 -0.03129871902766723 1104 5 -0.0858011205035254 1104 6 -0.17381517721880613 1104 7 -0.20497415972827326 1104 8 -0.2558230058292992 1104 9 -0.2801767495950145 1104 10 -0.35967979227649494 1104 11 -0.4343336859637361 1104 12 -0.38504464212251577 1104 13 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11 -1.1277824706018222 2314 12 -0.8113378152025419 2314 13 -0.6057389314975694 2314 14 -0.3870007189343596 2314 15 -0.5422834323030301 2314 16 -0.5901497134317637 2314 17 -0.6707514091866642 2314 18 -1.7346587177751118 2314 19 -1.2011771634569044 2314 20 -1.8748049973621335 2314 21 -1.1542942536881355 2314 22 -0.8177158358614562 2314 23 -0.6299526694020301 2314 24 -0.42756987546248837 2314 25 -0.50479689513746 2315 1 -2.4213022654902545 2315 2 -1.0005206008517682 2315 3 -0.33066028883320797 2315 4 -0.16844524040306652 2315 5 -0.13410310665102543 2315 6 0.007823681117955061 2315 7 0.12847524807957456 2315 8 0.018996194021219992 2315 9 0.09948615314448261 2315 10 0.02150382763338854 2315 11 -0.25962362443011244 2315 12 -0.12350209728874972 2315 13 -0.10415033493142545 2315 14 -0.2348700292414705 2315 15 -0.7035729936391135 2315 16 -0.5252303906136646 2315 17 -0.4679216021556343 2315 18 -0.24341678811536838 2315 19 -0.22429665618479078 2315 20 -0.1601631638946941 2315 21 -0.2592704827996499 2315 22 -0.2061237631868167 2315 23 -0.09239121944525408 2315 24 -0.040332138109455684 2315 25 0.13420436794851298 2316 1 1.2959786909062458 2316 2 0.5832906571051417 2316 3 -0.01741565998161576 2316 4 -0.22920999940100018 2316 5 -0.2906243785628462 2316 6 -0.38613615560211656 2316 7 -0.29083909562914556 2316 8 -0.41586377180044565 2316 9 -0.39039720767579666 2316 10 -0.5169817286601273 2316 11 -0.5335762971314679 2316 12 -0.4331012853666252 2316 13 -0.3575879445371797 2316 14 -0.5873372128119883 2316 15 -0.5685600461677893 2316 16 -0.5172323607188076 2316 17 -0.627792799069141 2316 18 -0.41769870717688207 2316 19 -0.5449223138193204 2316 20 -0.5541465821763794 2316 21 -0.5710508160757684 2316 22 -0.5421228079843203 2316 23 -0.37419461190661707 2316 24 -0.44617469387313236 2316 25 -0.5394275656649157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50108.0 3 3.0 4 425.0 5 2058.0 6 2490.0 7 2250.0 8 2109.0 9 2022.0 10 1942.0 11 1952.0 12 1969.0 13 1943.0 14 1958.0 15 2014.0 16 2015.0 17 2184.0 18 2548.0 19 3119.0 20 3605.0 21 4468.0 22 5642.0 23 6737.0 24 7950.0 25 9150.0 26 10233.0 27 11562.0 28 13404.0 29 16050.0 30 20549.0 31 26345.0 32 35295.0 33 48098.0 34 67283.0 35 98325.0 36 172310.0 37 291294.0 38 886170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.429430198738096 19.831517622298243 12.073521389907842 24.665530789055822 2 13.922310584384087 20.08373649987611 43.62630807239946 22.367644843340344 3 18.6100731337105 25.287098109562578 29.817579688146818 26.285249068580104 4 11.783233494598553 16.91546324841798 39.209524471803974 32.09177878517949 5 12.251821538855449 38.55520680894645 36.49826399235406 12.694707659844035 6 30.86105281157006 37.68653937908001 18.65468254865675 12.797725260693177 7 27.438148681696028 31.63228289023603 22.94932794132994 17.980240486738 8 23.938798489832866 38.961208133707196 20.804980902126154 16.29501247433378 9 26.696846058621425 14.70633016975632 20.88446287228667 37.712360899335586 10 15.285613909690257 28.681511287890572 35.33765501753272 20.695219784886458 11 33.6270723854586 21.605154480522597 26.641484831051322 18.126288302967488 12 24.412748299897572 24.052843414071535 32.82167085222719 18.7127374338037 13 29.63644398200839 22.56679960477165 25.97169957569776 21.8250568375222 14 19.860910683630554 23.655278708202594 27.976458413320447 28.5073521948464 15 21.76554608903924 33.98510031311096 23.241545976405426 21.007807621444375 16 20.492516023675595 28.03880817663223 30.5632069228024 20.905468876889778 17 20.514777062180357 29.715852079088005 29.12927829765415 20.64009256107749 18 20.42497215437548 26.79542307171116 32.960626121674636 19.818978652238723 19 23.458700630738775 26.30641817611875 28.32451782832821 21.910363364814263 20 23.602984709318285 27.427253774160082 29.40283763476781 19.566923881753826 21 21.895869097115416 26.676942379930164 27.963997517070144 23.46319100588428 22 22.999136510189675 29.563808149867004 27.898931465800125 19.538123874143192 23 20.711325921137117 29.024010573301528 29.58764785963457 20.677015645926787 24 23.27066562787983 26.47288617277911 30.273366215979685 19.983081983361373 25 21.76544315327622 27.984354259685922 29.859810322189347 20.39039226484851 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 885.0 1 885.0 2 1352.0 3 1819.0 4 1819.0 5 1819.0 6 3042.0 7 4265.0 8 4265.0 9 4265.0 10 4859.0 11 5453.0 12 5453.0 13 5453.0 14 7237.5 15 9022.0 16 9022.0 17 9022.0 18 15732.0 19 22442.0 20 22442.0 21 22442.0 22 36896.0 23 51350.0 24 51350.0 25 51350.0 26 76968.0 27 102586.0 28 102586.0 29 102586.0 30 134334.0 31 166082.0 32 166082.0 33 166082.0 34 183093.5 35 200105.0 36 200105.0 37 200105.0 38 206709.5 39 213314.0 40 213314.0 41 213314.0 42 219272.0 43 225230.0 44 225230.0 45 225230.0 46 229684.0 47 234138.0 48 234138.0 49 234138.0 50 219382.0 51 204626.0 52 204626.0 53 204626.0 54 175246.0 55 145866.0 56 145866.0 57 145866.0 58 124180.5 59 102495.0 60 102495.0 61 102495.0 62 84180.0 63 65865.0 64 65865.0 65 65865.0 66 50699.0 67 35533.0 68 35533.0 69 35533.0 70 25909.0 71 16285.0 72 16285.0 73 16285.0 74 11388.0 75 6491.0 76 6491.0 77 6491.0 78 4454.0 79 2417.0 80 2417.0 81 2417.0 82 1629.0 83 841.0 84 841.0 85 841.0 86 559.0 87 277.0 88 277.0 89 277.0 90 192.5 91 108.0 92 108.0 93 108.0 94 76.5 95 45.0 96 45.0 97 45.0 98 42.0 99 39.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34942085048297766 2 0.3007297069343341 3 0.3207013285254726 4 0.31123819102223343 5 0.295723046976225 6 0.25440434776150034 7 0.35772860491896086 8 0.3223518757644097 9 0.267553707431699 10 0.2419152069868765 11 0.25484449369188356 12 0.2530839099703507 13 0.27267040387240393 14 0.2633723210930584 15 0.35211674430657486 16 0.4096108064628828 17 0.4262263153348492 18 0.12175536799225785 19 0.13237388856275298 20 0.19437944650548888 21 0.19768054098336305 22 0.1568019876990216 23 0.25919093475441785 24 0.35717842250598186 25 0.3986621764446002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1817579.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.27683812833615 #Duplication Level Percentage of deduplicated Percentage of total 1 83.68174037412224 54.62479420699029 2 10.908453905843784 14.241387596843616 3 2.7696650890514447 5.423849390631446 4 1.0255366188555244 2.6777515145485293 5 0.47577727350544763 1.552861803387811 6 0.25308977886936396 0.9912540316315117 7 0.1618611901674023 0.7396050696872148 8 0.11771987147764286 0.6147504795947702 9 0.08242187673257771 0.4842215555135535 >10 0.44825547043120656 5.260773163639716 >50 0.03780064844349883 1.7106290698889617 >100 0.030143115346189277 4.12384832156124 >500 0.004233026490725242 1.8850453870612234 >1k 0.002878458013693164 3.687029407463021 >5k 4.2330264907252416E-4 1.982199001557079 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7838 0.43123297529295834 No Hit CTGTAGGACGTGGAATATGGCAAGA 7364 0.4051543289177527 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7306 0.40196327092247436 No Hit GTATCAACGCAGAGTACTTTTTTTT 7306 0.40196327092247436 No Hit CTTTAGGACGTGAAATATGGCGAGG 6054 0.33308043281750066 No Hit GGTATCAACGCAGAGTACTTTTTTT 4588 0.25242369107477586 No Hit TATCAACGCAGAGTACTTTTTTTTT 4223 0.2323420330010415 No Hit GTCCTACAGTGTGCATTTCTCATTT 3791 0.2085741527603477 No Hit CTGAAGGACCTGGAATATGGCGAGA 3431 0.18876758589310286 No Hit GTCCTTCAGTGTGCATTTCTCATTT 3046 0.16758556299341046 No Hit ATTTAGAAATGTCCACTGTAGGACG 2944 0.16197370238102443 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2569 0.14134186189431105 No Hit CTGTAGGACCTGGAATATGGCGAGA 2437 0.13407945404298793 No Hit GAATATGGCAAGAAAACTGAAAATC 2368 0.1302831953934327 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2143 0.11790409110140466 No Hit GGAATATGGCGAGAAAACTGAAAAT 2110 0.11608848913857389 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2053 0.11295244938459346 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1893 0.1041495307769291 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 5.501824129790232E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCGCA 55 1.9460342E-4 13.8301935 16 CATTTCG 55 1.946491E-4 13.829812 14 GGTATCA 1220 0.0 13.782566 1 ATTTCGC 50 0.0014902356 13.311194 15 TAGGACC 1030 0.0 12.72936 4 GTCCTAG 215 0.0 12.371895 1 AATGTCC 905 0.0 12.260221 8 ACACCGT 95 1.0392341E-6 12.000803 6 AAATGTC 960 0.0 11.479936 7 TAGGACT 350 0.0 11.401078 4 GTGGTAT 325 0.0 11.107514 1 TAGAAAT 1030 0.0 11.069008 4 TGTCGAG 95 1.35733535E-5 11.002257 18 GAAATGT 1030 0.0 10.976462 6 TAAGCGA 165 8.0035534E-11 10.941033 13 ATGTCCA 1035 0.0 10.908057 9 GGGCCGT 70 0.0014907746 10.857869 6 GTATTAG 140 8.352799E-9 10.856969 1 GGCGAGG 1780 0.0 10.8412895 19 GTCCTAA 1765 0.0 10.710876 1 >>END_MODULE