FastQCFastQC Report
Thu 26 May 2016
SRR1512282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817579
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83820.46116289855901726No Hit
CTGTAGGACGTGGAATATGGCAAGA82170.45208488874486336No Hit
GTCCTACAGTGGACATTTCTAAATT78550.43216828539502267No Hit
GTCCTAAAGTGTGTATTTCTCATTT73170.4025684715767513No Hit
CTTTAGGACGTGAAATATGGCGAGG65000.3576185684363651No Hit
GGTATCAACGCAGAGTACTTTTTTT54900.30205014472548375No Hit
TATCAACGCAGAGTACTTTTTTTTT53840.2962182111479061No Hit
CTGAAGGACCTGGAATATGGCGAGA39230.21583656061167078No Hit
GTCCTACAGTGTGCATTTCTCATTT37890.20846411627775188No Hit
GTCCTTCAGTGTGCATTTCTCATTT30340.16692534409783563No Hit
ATTTAGAAATGTCCACTGTAGGACG30080.16549486982409017No Hit
TTTCTAAATTTTCCACCTTTTTCAG27740.15262060136038103No Hit
CTGTAGGACCTGGAATATGGCGAGA26370.1450831023025684No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26270.14453291988958938No Hit
GAATATGGCAAGAAAACTGAAAATC25270.13903109575979916No Hit
GGAATATGGCGAGAAAACTGAAAAT22540.12401111588547184No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22380.1231308240247054No Hit
ATTCCAGGTCCTTCAGTGTGCATTT20750.11416285069314731No Hit
TTCCAGGTCCTTCAGTGTGCATTTC19370.10657033339403679No Hit
GCCATATTCCACGTCCTACAGTGGA19050.10480974967250392No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18680.10277407474448153No Hit
ACAGTGGACATTTCTAAATTTTCCA18270.10051832685126755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGA250.00605572218.98734517
CGTGCAA400.005270123514.2538419
TAGGACC10400.013.8883584
GGTATCA14150.013.2395581
GCGTTGA655.401868E-513.1671831
TACACCG604.082372E-412.670435
CCACCTT9050.012.2810213
CACCTTT8750.012.04704814
TAGAAAT9650.011.9151294
AAATGTC9500.011.9048477
GTCCCGC1603.6379788E-1211.87493112
ACCTTTT9000.011.60529215
CTTAGAC1157.048948E-811.5683363
GGACCTG22850.011.56269556
AATGTCC9550.011.5439678
AGGACCT22400.011.4967185
TGTCGAG2200.011.21948518
GTCCTAT2650.011.1244751
TTCCACC9650.011.12428911
TTGCGAG600.00590673111.07564618
Produced by FastQC (version 0.11.5)