FastQCFastQC Report
Thu 26 May 2016
SRR1512281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912290
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74250.38827792855686116No Hit
GTCCTACAGTGGACATTTCTAAATT63010.3295002327052905No Hit
GTCCTAAAGTGTGTATTTCTCATTT60860.3182571681073477No Hit
CTGTAGGACGTGGAATATGGCAAGA59390.3105700495217775No Hit
CTTTAGGACGTGAAATATGGCGAGG52960.27694544237537194No Hit
GGTATCAACGCAGAGTACTTTTTTT49030.2563941661568067No Hit
TATCAACGCAGAGTACTTTTTTTTT43890.2295153977691668No Hit
CTGAAGGACCTGGAATATGGCGAGA28500.14903597257738105No Hit
GTCCTACAGTGTGCATTTCTCATTT28120.1470488262763493No Hit
GTCCTTCAGTGTGCATTTCTCATTT25350.1325635756083021No Hit
ATTTAGAAATGTCCACTGTAGGACG22980.12017005788870934No Hit
TTTCTAAATTTTCCACCTTTTTCAG21620.11305816586396414No Hit
GAATATGGCAAGAAAACTGAAAATC21430.11206459271344826No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21170.11070496629695285No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21100.11033891303097332No Hit
CTGTAGGACCTGGAATATGGCGAGA19890.10401142086189856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.013.8420521
TCCAACG1450.013.76006718
GCCGGTT1102.7739588E-912.94603911
CCAACGA905.3744407E-712.67299519
AGCTCGT550.003089476612.08011510
CGACTAA1459.640644E-1111.80551317
TACACCG658.017802E-411.6935365
TAGGACC8550.011.6678934
CGGTTTC1251.8295395E-811.40119813
CACCTTT7950.011.36295114
GGCGAGG14000.011.13413119
AATGTCC7900.010.9229448
ACCTTTT8300.010.76922415
AAATGTC8150.010.7250547
GTTAGAC803.7660208E-410.6891853
GTATCAA27500.010.676511
CCACCTT8400.010.6320713
GTCCTAG2600.010.5981031
CCTACAC2250.010.55721953
TATACAG3450.010.4648695
Produced by FastQC (version 0.11.5)