##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512281_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1912290 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.131029812423847 31.0 30.0 34.0 16.0 34.0 2 30.004364400796952 31.0 30.0 34.0 23.0 34.0 3 30.657985451997344 33.0 31.0 34.0 26.0 34.0 4 34.31909961355234 37.0 35.0 37.0 32.0 37.0 5 34.48718395222482 37.0 35.0 37.0 32.0 37.0 6 34.64221535436571 37.0 35.0 37.0 32.0 37.0 7 34.58507653127925 37.0 35.0 37.0 32.0 37.0 8 34.58424036103311 37.0 35.0 37.0 32.0 37.0 9 36.17833749065257 39.0 37.0 39.0 32.0 39.0 10 36.11405801421333 39.0 37.0 39.0 32.0 39.0 11 36.1338865966982 39.0 37.0 39.0 32.0 39.0 12 36.07840128850749 39.0 37.0 39.0 32.0 39.0 13 36.055342024483735 39.0 37.0 39.0 32.0 39.0 14 37.29508442757113 40.0 38.0 41.0 32.0 41.0 15 37.22750471947246 40.0 38.0 41.0 32.0 41.0 16 37.1598742868498 40.0 38.0 41.0 31.0 41.0 17 37.12856209047791 40.0 38.0 41.0 31.0 41.0 18 36.915456860622605 40.0 38.0 41.0 31.0 41.0 19 37.02441313817465 40.0 38.0 41.0 31.0 41.0 20 36.96769789101025 40.0 38.0 41.0 31.0 41.0 21 36.91308797305848 40.0 38.0 41.0 31.0 41.0 22 36.839297386902615 40.0 38.0 41.0 31.0 41.0 23 36.71828174596949 40.0 38.0 41.0 31.0 41.0 24 36.600020394396246 40.0 38.0 41.0 30.0 41.0 25 36.48712642956874 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9199169844400323 1101 2 0.10243809098005485 1101 3 -0.33511714327538655 1101 4 -0.5091522605267471 1101 5 -0.8137923867742387 1101 6 -0.7846095846527845 1101 7 -0.7675771654776611 1101 8 -0.9534949827232779 1101 9 -1.0758072119524655 1101 10 -1.0252675833905442 1101 11 -1.13425386326508 1101 12 -1.1554477228195736 1101 13 -1.172160815286162 1101 14 -1.4527392493879532 1101 15 -1.6741112868714794 1101 16 -1.7368567633354033 1101 17 -1.6694853134130412 1101 18 -1.5035898467103124 1101 19 -1.6686106161068466 1101 20 -1.6767103267631924 1101 21 -1.687802650045306 1101 22 -1.6425926893264204 1101 23 -1.8588839512556277 1101 24 -1.9681293473557773 1101 25 -1.8878714629170688 1102 1 0.7703510070255639 1102 2 0.09681795321099429 1102 3 -0.3298137791273241 1102 4 -0.36024760145206614 1102 5 -0.4483610558489559 1102 6 -0.5984693766197964 1102 7 -0.5664890877625339 1102 8 -0.6214933396508542 1102 9 -0.8371073021627637 1102 10 -0.7596785370439392 1102 11 -0.6924816155639775 1102 12 -0.9996024255345475 1102 13 -0.9489486331126429 1102 14 -1.4048649285591424 1102 15 -1.3582577742243416 1102 16 -1.5527652078243364 1102 17 -1.510665762071909 1102 18 -1.4072076578106945 1102 19 -1.4329820066270713 1102 20 -1.6506262468654427 1102 21 -1.6742448611373817 1102 22 -1.557318939544622 1102 23 -1.7251920554112843 1102 24 -1.682007083724443 1102 25 -1.7189792428527895 1103 1 0.8378173311914416 1103 2 0.018910991280236544 1103 3 -0.4573105855545414 1103 4 -0.5176971094160194 1103 5 -0.6684184880427182 1103 6 -0.7767900263830185 1103 7 -0.8101187352361094 1103 8 -0.7980793842879095 1103 9 -1.0410109888718253 1103 10 -0.9940650641623847 1103 11 -0.9645421677885935 1103 12 -1.09956038502974 1103 13 -1.1999961713110139 1103 14 -1.4609079326369567 1103 15 -1.5240076760719177 1103 16 -1.5790078058580477 1103 17 -1.553135357985063 1103 18 -1.3983953657693249 1103 19 -1.5712098545817383 1103 20 -1.5645787144577739 1103 21 -1.5548365088587985 1103 22 -1.7574734355044512 1103 23 -1.7084534489092462 1103 24 -1.6246345342052493 1103 25 -1.6356463920780513 1104 1 1.2651739235864774 1104 2 0.49211159575930097 1104 3 -0.19194227728243618 1104 4 -0.008809140442906482 1104 5 -0.3278998165750906 1104 6 -0.2947157514205614 1104 7 -0.44950499408208344 1104 8 -0.4351987078221953 1104 9 -0.49814370023473487 1104 10 -0.5721746594484927 1104 11 -0.6464146535796473 1104 12 -0.690826256700376 1104 13 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1108 21 -1.1656932175532049 1108 22 -1.388401784059809 1108 23 -1.3090922894450046 1108 24 -1.4580321043681863 1108 25 -1.4844834421964634 1109 1 0.6545028655213443 1109 2 0.15738458657260068 1109 3 -0.4314677790371597 1109 4 -0.3644835124153687 1109 5 -0.5282714467133758 1109 6 -0.5148504340432467 1109 7 -0.475501007339723 1109 8 -0.5139746959811049 1109 9 -0.6999281080054374 1109 10 -0.7035685087259012 1109 11 -0.6981834908870255 1109 12 -0.7204867954660514 1109 13 -0.6522820573097263 1109 14 -0.9649209775165346 1109 15 -0.81390271991053 1109 16 -0.9336803071786122 1109 17 -0.9544375235042324 1109 18 -0.9342702038759967 1109 19 -1.007250282560726 1109 20 -1.2439400025544742 1109 21 -1.286756904953286 1109 22 -1.2708255220994147 1109 23 -1.3913631651407954 1109 24 -1.254243768427564 1109 25 -1.459957782581391 1110 1 0.6335290805868752 1110 2 0.15967682778034487 1110 3 -0.12694397882108532 1110 4 -0.07935846976902639 1110 5 -0.04749317751540616 1110 6 -0.16406267603284164 1110 7 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16 1.1551085849588247 1201 17 1.0816751065103958 1201 18 1.258305114743223 1201 19 1.07267695801837 1201 20 1.0798420009720218 1201 21 1.1194827650333536 1201 22 1.1378816098772901 1201 23 1.19170672193966 1201 24 1.2931444300232613 1201 25 1.12036001475591 1202 1 2.48110364185629 1202 2 1.7573746387134506 1202 3 1.319191141027268 1202 4 0.993572846728469 1202 5 0.9042913629639884 1202 6 0.8952834701699217 1202 7 0.7962167266786366 1202 8 0.8354631997351873 1202 9 0.9884549529005824 1202 10 0.9669958262898604 1202 11 1.0196025276481464 1202 12 1.002266872430198 1202 13 1.1351799222740482 1202 14 1.2417498156694862 1202 15 1.2152965075728943 1202 16 1.1450770946817386 1202 17 1.1320390151231052 1202 18 1.35421670665054 1202 19 1.2381940501238233 1202 20 1.105402182698782 1202 21 1.2334751816151979 1202 22 1.2182536879802797 1202 23 1.2363125828829453 1202 24 1.283558598760976 1202 25 1.385232427519405 1203 1 2.614563495499631 1203 2 1.7664631202789955 1203 3 1.2649421143011779 1203 4 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-0.5479549758252205 2314 14 -0.5358468381742014 2314 15 -0.5701561568583955 2314 16 -0.8441530819593623 2314 17 -0.8897203835959147 2314 18 -2.2121725951306814 2314 19 -1.495740597112487 2314 20 -1.8275843210715692 2314 21 -1.1835718946955396 2314 22 -0.8756107867630547 2314 23 -0.6467299699933378 2314 24 -0.5532046764821104 2314 25 -0.4554439148196465 2315 1 -2.2070528577308615 2315 2 -0.988535660709772 2315 3 -0.46254504897247983 2315 4 -0.35014685656531697 2315 5 -0.18435287338684248 2315 6 -0.10898370619408837 2315 7 -0.19238130542139942 2315 8 -0.1611206751704941 2315 9 -0.20333980032482657 2315 10 -0.1419060034892894 2315 11 -0.36841876490536407 2315 12 -0.24480780438602068 2315 13 -0.34662975556766185 2315 14 -0.31095178963495584 2315 15 -0.8223497396656398 2315 16 -0.714629619957627 2315 17 -0.6103686917913365 2315 18 -0.480335747850944 2315 19 -0.37503020434542833 2315 20 -0.3719921700141029 2315 21 -0.41040073064440463 2315 22 -0.4434530539048396 2315 23 -0.37521373159204074 2315 24 -0.28193072355396964 2315 25 -0.11105041969172902 2316 1 1.3598316074439438 2316 2 0.4966620673104565 2316 3 0.004181425126684957 2316 4 -0.061723283734700374 2316 5 -0.23943489713198574 2316 6 -0.3397768857144854 2316 7 -0.31825649247164023 2316 8 -0.3168036379436785 2316 9 -0.41188220968602707 2316 10 -0.4802462161637635 2316 11 -0.5463587137028512 2316 12 -0.510009580826214 2316 13 -0.5417760297375835 2316 14 -0.5871507827188651 2316 15 -0.5341548025688425 2316 16 -0.4644146996652907 2316 17 -0.48190094848278164 2316 18 -0.2632144987539391 2316 19 -0.59319301622984 2316 20 -0.5211306375979632 2316 21 -0.4475589019801376 2316 22 -0.5979579751133457 2316 23 -0.5614732104485043 2316 24 -0.6698220561786954 2316 25 -0.665398295717111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49922.0 3 3.0 4 391.0 5 2003.0 6 2444.0 7 2265.0 8 2050.0 9 2032.0 10 1964.0 11 1970.0 12 1980.0 13 1957.0 14 2076.0 15 2078.0 16 2135.0 17 2360.0 18 2622.0 19 3269.0 20 3923.0 21 4739.0 22 5715.0 23 6865.0 24 8167.0 25 9757.0 26 10889.0 27 12123.0 28 13883.0 29 17133.0 30 21471.0 31 28040.0 32 37207.0 33 50420.0 34 70948.0 35 102740.0 36 178211.0 37 302385.0 38 944153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.756706962226914 19.59180739152497 12.217546698823353 24.433938947424764 2 14.33478515366847 20.062499606623827 42.69967250122209 22.903042738485617 3 18.638841236615832 25.184479873253697 29.873516003294565 26.303162886835906 4 12.152109883365638 17.064181684182365 38.79956042457321 31.984148007878787 5 12.715517015315514 38.175291892668675 36.066510934633456 13.042680157382359 6 31.0161683763314 37.3344747990683 18.60314790806133 13.046208916538967 7 27.36033437458014 31.634746993819697 22.867280918312506 18.137637713287653 8 24.34863536590996 37.94193747403145 20.894923384913692 16.8145037751449 9 26.649682049647232 14.760991937987097 20.80446053304922 37.78486547931645 10 15.546925247425367 28.57590655364679 34.88554824093551 20.991619957992334 11 33.54786215631874 21.74175175471156 26.171100891458483 18.539285197511212 12 24.14802737636207 24.163650041808978 32.37330830910859 19.31501427272036 13 29.408054212802238 22.448196288600116 25.90203921975764 22.241710278840007 14 20.375067633599954 23.15129583880344 27.90426018278898 28.56937634480763 15 22.33169991404345 32.819448219838854 23.34932467960458 21.499527186513113 16 21.132877355369818 27.726810181057488 29.590365443969425 21.549947019603273 17 20.961265371801176 29.210098438346844 28.64427750958965 21.18435868026233 18 20.903271811904848 26.61464954146885 31.90959009681615 20.57248854981016 19 23.48703856620209 26.37915027953467 28.124795460840797 22.00901569342244 20 23.720778656014655 27.122919048402462 29.04409568788372 20.112206607699168 21 22.43174379290164 26.462958606420163 27.767330807547598 23.337966793130605 22 23.28899419390858 28.76969100867614 27.730083262891398 20.21123153452388 23 21.449018538713197 28.35159592316081 29.01271915107793 21.186666387048067 24 23.285022836274173 26.456019114982205 29.62370921128657 20.635248837457052 25 22.22345304063188 27.551980932231196 29.29616728397168 20.92839874316524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 956.0 1 956.0 2 1475.5 3 1995.0 4 1995.0 5 1995.0 6 3131.0 7 4267.0 8 4267.0 9 4267.0 10 4868.5 11 5470.0 12 5470.0 13 5470.0 14 7419.5 15 9369.0 16 9369.0 17 9369.0 18 16510.0 19 23651.0 20 23651.0 21 23651.0 22 37557.0 23 51463.0 24 51463.0 25 51463.0 26 76837.0 27 102211.0 28 102211.0 29 102211.0 30 133171.0 31 164131.0 32 164131.0 33 164131.0 34 180749.0 35 197367.0 36 197367.0 37 197367.0 38 206208.5 39 215050.0 40 215050.0 41 215050.0 42 224061.0 43 233072.0 44 233072.0 45 233072.0 46 239709.5 47 246347.0 48 246347.0 49 246347.0 50 234290.5 51 222234.0 52 222234.0 53 222234.0 54 194815.5 55 167397.0 56 167397.0 57 167397.0 58 142600.5 59 117804.0 60 117804.0 61 117804.0 62 97273.5 63 76743.0 64 76743.0 65 76743.0 66 58928.5 67 41114.0 68 41114.0 69 41114.0 70 30206.5 71 19299.0 72 19299.0 73 19299.0 74 13513.5 75 7728.0 76 7728.0 77 7728.0 78 5371.5 79 3015.0 80 3015.0 81 3015.0 82 2037.5 83 1060.0 84 1060.0 85 1060.0 86 699.0 87 338.0 88 338.0 89 338.0 90 229.0 91 120.0 92 120.0 93 120.0 94 88.5 95 57.0 96 57.0 97 57.0 98 44.5 99 32.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3462340962929263 2 0.29901322498156657 3 0.32003514111353404 4 0.30879207651559126 5 0.29263343948878046 6 0.2510602471382478 7 0.35988265378159173 8 0.32097642094033857 9 0.2653363245114496 10 0.2375685696207165 11 0.25383179329495004 12 0.2512694204330933 13 0.26873539055268814 14 0.25906112566608624 15 0.3491102290970512 16 0.40851544483315816 17 0.4263997615424439 18 0.1191241914144821 19 0.1312039491918067 20 0.19181191137327497 21 0.1939559376454408 22 0.1524873319423309 23 0.25780608589701354 24 0.35397350820220785 25 0.3924613944537701 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1912290.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.80177227154056 #Duplication Level Percentage of deduplicated Percentage of total 1 83.42306252478451 55.728084249751454 2 11.266053235516289 15.05184645276024 3 2.731916929086907 5.4749067768489 4 1.0063355967834915 2.689000054603026 5 0.45319200025983836 1.5137014398320836 6 0.26504368399870226 1.0623232692294882 7 0.1665172902314508 0.7786555070920781 8 0.12081113661595037 0.6456318428867753 9 0.07712381670126847 0.46368068759911557 >10 0.42307510099809836 5.130377190835805 >50 0.03391074598445784 1.5685827476847454 >100 0.026587791769025627 3.7153563311823117 >500 0.003303039325100618 1.4509765330467301 >1k 0.002673888977462401 3.095808868509997 >5k 3.9321896727388255E-4 1.6310680481372162 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7425 0.38827792855686116 No Hit GTCCTACAGTGGACATTTCTAAATT 6301 0.3295002327052905 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6086 0.3182571681073477 No Hit CTGTAGGACGTGGAATATGGCAAGA 5939 0.3105700495217775 No Hit CTTTAGGACGTGAAATATGGCGAGG 5296 0.27694544237537194 No Hit GGTATCAACGCAGAGTACTTTTTTT 4903 0.2563941661568067 No Hit TATCAACGCAGAGTACTTTTTTTTT 4389 0.2295153977691668 No Hit CTGAAGGACCTGGAATATGGCGAGA 2850 0.14903597257738105 No Hit GTCCTACAGTGTGCATTTCTCATTT 2812 0.1470488262763493 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2535 0.1325635756083021 No Hit ATTTAGAAATGTCCACTGTAGGACG 2298 0.12017005788870934 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2162 0.11305816586396414 No Hit GAATATGGCAAGAAAACTGAAAATC 2143 0.11206459271344826 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2117 0.11070496629695285 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2110 0.11033891303097332 No Hit CTGTAGGACCTGGAATATGGCGAGA 1989 0.10401142086189856 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.229332371136177E-5 0.0 3 0.0 0.0 0.0 1.0458664742272354E-4 0.0 4 0.0 0.0 0.0 1.0458664742272354E-4 0.0 5 0.0 0.0 0.0 1.0458664742272354E-4 0.0 6 0.0 0.0 0.0 1.568799711340853E-4 0.0 7 0.0 0.0 0.0 1.568799711340853E-4 0.0 8 0.0 0.0 0.0 1.568799711340853E-4 0.0 9 0.0 0.0 0.0 2.0917329484544707E-4 0.0 10 0.0 0.0 0.0 2.614666185568089E-4 0.0 11 0.0 0.0 0.0 5.229332371136178E-4 0.0 12 0.0 0.0 0.0 5.229332371136178E-4 0.0 13 0.0 0.0 0.0 5.229332371136178E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1215 0.0 13.842052 1 TCCAACG 145 0.0 13.760067 18 GCCGGTT 110 2.7739588E-9 12.946039 11 CCAACGA 90 5.3744407E-7 12.672995 19 AGCTCGT 55 0.0030894766 12.080115 10 CGACTAA 145 9.640644E-11 11.805513 17 TACACCG 65 8.017802E-4 11.693536 5 TAGGACC 855 0.0 11.667893 4 CGGTTTC 125 1.8295395E-8 11.401198 13 CACCTTT 795 0.0 11.362951 14 GGCGAGG 1400 0.0 11.134131 19 AATGTCC 790 0.0 10.922944 8 ACCTTTT 830 0.0 10.769224 15 AAATGTC 815 0.0 10.725054 7 GTTAGAC 80 3.7660208E-4 10.689185 3 GTATCAA 2750 0.0 10.67651 1 CCACCTT 840 0.0 10.63207 13 GTCCTAG 260 0.0 10.598103 1 CCTACAC 225 0.0 10.5572195 3 TATACAG 345 0.0 10.464869 5 >>END_MODULE