FastQCFastQC Report
Thu 26 May 2016
SRR1512281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85470.44695103776100903No Hit
CTGTAGGACGTGGAATATGGCAAGA64660.3381286311176652No Hit
GTCCTACAGTGGACATTTCTAAATT63930.3343112184867358No Hit
GTCCTAAAGTGTGTATTTCTCATTT62070.3245846602764225No Hit
GGTATCAACGCAGAGTACTTTTTTT58440.3056021837691982No Hit
CTTTAGGACGTGAAATATGGCGAGG57390.3001113847795052No Hit
TATCAACGCAGAGTACTTTTTTTTT54920.28719493382279887No Hit
CTGAAGGACCTGGAATATGGCGAGA33110.17314319480831883No Hit
GTCCTACAGTGTGCATTTCTCATTT30900.16158637026810785No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25990.13591034832582924No Hit
GTCCTTCAGTGTGCATTTCTCATTT25690.13434154861448838No Hit
ATTTAGAAATGTCCACTGTAGGACG24920.13031496268871354No Hit
TTTCTAAATTTTCCACCTTTTTCAG24360.12738653656087728No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22610.11823520491138896No Hit
GAATATGGCAAGAAAACTGAAAATC22320.11671869852375948No Hit
CTGTAGGACCTGGAATATGGCGAGA21310.11143707282891192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT250.00603819118.99693910
GGTATCA15050.014.0959051
AGCCGTC500.00149390813.30691057
TAGGACC7900.012.8720884
TCCAACG1551.8189894E-1212.24871818
GATAGAC550.003059971612.0956083
TCGAAAC752.0875153E-411.39220216
AATGTCC7900.011.309678
CGACTAA1701.2732926E-1111.16882417
ATGTCCA7950.011.1175269
CGTGCCA600.005883548411.08154810
GGCGAAA600.005908804511.07517119
AAATGTC7900.011.0690397
ACCGGGG951.3704979E-510.99276315
CCAACGA951.3716903E-510.991899519
TAGAAAT8600.010.9402964
CGGTTTC1053.4769346E-610.85454213
GGCGAGG14400.010.81147819
GAAATGT8200.010.7799876
TGTAGGA33700.010.6875692
Produced by FastQC (version 0.11.5)