FastQCFastQC Report
Thu 26 May 2016
SRR1512280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487064
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT174323.5789957787888245No Hit
GGTATCAACGCAGAGTACTTTTTTT109592.250012318709656No Hit
TATCAACGCAGAGTACTTTTTTTTT102262.099518749076097No Hit
GTACTGGTTCACTATCGGTCAGTCA93731.9243877601300856No Hit
GGATACCACGTGTCCCGCCCTACTC55801.1456399980290066No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54051.1097104281983476No Hit
ATCATTAACTGAATCCATAGGTTAA53751.1035510733702347No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48620.9982261058095034No Hit
GTACATGGGGGTTAAGCGACTAAGC47140.9678399553241464No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38630.7931195900333426No Hit
GTATTTAGCCTTGGAGGATGGTCCC38440.7892186653088711No Hit
GGTTAATGAGGCGAACCGGGGGAAC35130.721260450372025No Hit
ATACAGGGTGACAGCCCCGTACACA34430.7068886224397615No Hit
GTGGATGCCCTGGCAGTCAGAGGCG33210.6818405794721022No Hit
GTTCACTATCGGTCAGTCAGGAGTA31380.6442685150206133No Hit
CTATCGGTCAGTCAGGAGTATTTAG30710.6305126225711611No Hit
CCCATATTCAGACAGGATACCACGT30670.629691375260746No Hit
TATTCAGACAGGATACCACGTGTCC30390.6239426440878406No Hit
GTATCAACGCAGAGTACATGGGGGT29930.6144983000180675No Hit
AGCGTACACGGTGGATGCCCTGGCA28740.5900661925332195No Hit
GCCTTGGAGGATGGTCCCCCCATAT27330.5611172248410887No Hit
GTCAGGAGTATTTAGCCTTGGAGGA26240.5387382356322783No Hit
ACCCTGTATCGCGCGCCTTTCCAGA25640.5264195259760523No Hit
GGTATCAACGCAGAGTACATGGGGG25110.5155379991130529No Hit
TCTAAGTACCCCGAGGAAAAGAAAT23470.48186685938603546No Hit
CGGTACTGGTTCACTATCGGTCAGT22770.467495031453772No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22370.4592825583496214No Hit
GTTAATGATAGTGTGTCGAAACACA22200.45579225728035744No Hit
GGTTCACTATCGGTCAGTCAGGAGT20930.4297176551746793No Hit
GCCCAGAGCCTGAATCAGTGTGTGT20760.42622735410541535No Hit
ACTTAGATGTTTCAGTTCCCCCGGT20680.42458485948458524No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG20270.41616707455283086No Hit
TCACGGTACTGGTTCACTATCGGTC19780.4061067950002464No Hit
GAGTACATGGGGGTTAAGCGACTAA19620.40282180575858617No Hit
GTTATAACCGGCGATTTCCGAATGG19310.39645713910286945No Hit
GTGCTAATCTGCGATAAGCGTCGGT19080.39173496706798283No Hit
GTGGTATCAACGCAGAGTACATGGG18850.38701279503309627No Hit
ACGCAGAGTACATGGGGGTTAAGCG18220.3740781498940591No Hit
TATCAACGCAGAGTACATGGGGGTT17810.36566036496230475No Hit
GATATGAACCGTTATAACCGGCGAT17590.36114350475502194No Hit
GGTGATATGAACCGTTATAACCGGC17480.3588850746513805No Hit
CATCTAAGTACCCCGAGGAAAAGAA17190.3529310316508713No Hit
GGAGTATTTAGCCTTGGAGGATGGT16990.34882479509879605No Hit
GCCTGAATCAGTGTGTGTGTTAGTG16610.34102294564985297No Hit
CACTATCATTAACTGAATCCATAGG16030.32911485964883463No Hit
GAATCAGTGTGTGTGTTAGTGGAAG15380.31576959085458994No Hit
GTGGTATCAACGCAGAGTACTTTTT14960.30714649409523187No Hit
GTACTTAGATGTTTCAGTTCCCCCG14880.3055039994744017No Hit
ATCAACGCAGAGTACTTTTTTTTTT14760.30304025754315655No Hit
ATCCATAGGTTAATGAGGCGAACCG14600.29975526830149635No Hit
GTCCCGCCCTACTCATCGAGCTCAC14300.2935959134733834No Hit
CTATCATTAACTGAATCCATAGGTT14250.2925693543353646No Hit
GCGTACACGGTGGATGCCCTGGCAG13820.2837409457484027No Hit
CCGTTATAACCGGCGATTTCCGAAT13160.2701903651265542No Hit
GCCCTACTCATCGAGCTCACAGCAT13150.26998505329895045No Hit
CACACACACTGATTCAGGCTCTGGG12840.26362038664323373No Hit
TCATTAACCTATGGATTCAGTTAAT12500.2566397845047057No Hit
TCGGTAAGGTGATATGAACCGTTAT11810.242473268400046No Hit
AGCTCACAGCATGTGCATTTTTGTG10860.22296864477768835No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG10840.2225580211224808No Hit
CCCCAGTAGCGGCGAGCGAACGGGG10680.2192730318808206No Hit
CATTAACTGAATCCATAGGTTAATG10630.2182464727428018No Hit
GGGTACTTAGATGTTTCAGTTCCCC10320.21188180608708507No Hit
GTCCCCCCATATTCAGACAGGATAC10300.2114711824318775No Hit
GTGTTTCGACACACTATCATTAACT10290.21126587060427376No Hit
AGTCAGGAGTATTTAGCCTTGGAGG10230.21003399963865121No Hit
TTTGTGTACGGGGCTGTCACCCTGT10050.20633838674178345No Hit
GGGAAACCCAGTGTGTTTCGACACA9880.20284808567251944No Hit
GGTATCAACGCAGAGTACATGGGGA9820.20161621470689683No Hit
CCTATGGATTCAGTTAATGATAGTG9660.19833122546523663No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA9550.1960727953615952No Hit
GATTCAGGCTCTGGGCTGCTCCCCG9520.19545685987878392No Hit
TTCCAGACGCTTCCACTAACACACA9390.19278780611993496No Hit
GTACATGGGCATCTAAGTACCCCGA9390.19278780611993496No Hit
CCCTGGCAGTCAGAGGCGATGAAGG9350.1919665588095199No Hit
GGGTGACAGCCCCGTACACAAAAAT9130.18744969860223706No Hit
ATTTAGCCTTGGAGGATGGTCCCCC8940.18354877387776555No Hit
TAAGTACCCCGAGGAAAAGAAATCA8820.18108503194652037No Hit
GGGGTTAAGCGACTAAGCGTACACG8570.17595223625642625No Hit
GTGGTATCCTGTCTGAATATGGGGG8230.16897163411789826No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA8170.16773976315227568No Hit
GGGGAAACCCAGTGTGTTTCGACAC8100.16630258035904932No Hit
GTGCATTTTTGTGTACGGGGCTGTC8070.16568664487623802No Hit
GTATCGCGCGCCTTTCCAGACGCTT7960.16342821477259661No Hit
GGAGGATGGTCCCCCCATATTCAGA7840.16096447284135146No Hit
CCACGTGTCCCGCCCTACTCATCGA7830.16075916101374768No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC7750.15911666639291758No Hit
AAGTACCCCGAGGAAAAGAAATCAA7710.1582954190825025No Hit
CACTGATTCAGGCTCTGGGCTGCTC7700.15809010725489875No Hit
AGGTTAATGAGGCGAACCGGGGGAA7620.15644761263406862No Hit
GTACGGGGCTGTCACCCTGTATCGC7500.15398387070282346No Hit
CAGTAGCGGCGAGCGAACGGGGAGC7090.1455660857710691No Hit
TCCCCCAGTAGCGGCGAGCGAACGG7080.14536077394346533No Hit
ATTCAGACAGGATACCACGTGTCCC7020.14412890297784275No Hit
ATGATAGTGTGTCGAAACACACTGG6970.14310234383982393No Hit
GCATGTGCATTTTTGTGTACGGGGC6950.1426917201846164No Hit
TAATGATAGTGTGTCGAAACACACT6930.14228109652940887No Hit
GTATCAACGCAGAGTACATGGGCAT6870.1410492255637863No Hit
GTCAGAGGCGATGAAGGACGTGCTA6840.140433290080975No Hit
GGGTTTCCCCATTCGGAAATCGCCG6790.13940673094295616No Hit
GGTATCAACGCAGAGTACATGGGCA6790.13940673094295616No Hit
CGGTGGATGCCCTGGCAGTCAGAGG6670.13694298901171098No Hit
GGGCTGTCACCCTGTATCGCGCGCC6610.1357111180460884No Hit
ACCCAGTGTGTTTCGACACACTATC6310.12955176321797546No Hit
GTACATGGGATAGGTTAATGAGGCG6250.12831989225235285No Hit
AACACACACACTGATTCAGGCTCTG6190.12708802128673027No Hit
GAATATGGGGGGACCATCCTCCAAG6090.12503490301069264No Hit
GACTAAGCGTACACGGTGGATGCCC6090.12503490301069264No Hit
ACATGGGGGTTAAGCGACTAAGCGT6080.12482959118308888No Hit
GAGTAGGGCGGGACACGTGGTATCC6030.12380303204507005No Hit
GCGATAAGCGTCGGTAAGGTGATAT5990.12298178473465499No Hit
GTACATGGGGAGCGAACGGGGAGCA5990.12298178473465499No Hit
CCCTCACGGTACTGGTTCACTATCG5890.12092866645861734No Hit
GGATGGTCCCCCCATATTCAGACAG5890.12092866645861734No Hit
CATTAACCTATGGATTCAGTTAATG5880.12072335463101358No Hit
GCATTTTTGTGTACGGGGCTGTCAC5850.12010741914820228No Hit
CACGTGTCCCGCCCTACTCATCGAG5820.119491483665391No Hit
AATCTGCGATAAGCGTCGGTAAGGT5810.11928617183778722No Hit
CTAACACACACACTGATTCAGGCTC5770.11846492452737217No Hit
GTATCAACGCAGAGTACATGGGGAA5710.1172330535617496No Hit
GATGAAGGACGTGCTAATCTGCGAT5680.11661711807893829No Hit
GTCTGAATATGGGGGGACCATCCTC5660.11620649442373077No Hit
CTGTCACCCTGTATCGCGCGCCTTT5630.11559055894091946No Hit
GGTATCAACGCAGAGTACATGGGAT5610.11517993528571194No Hit
TATTTAGCCTTGGAGGATGGTCCCC5520.11333212883727806No Hit
GTATCAACGCAGAGTACATGGGGAG5460.11210025787165548No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT5390.11066307507842912No Hit
GAGCAGCCCAGAGCCTGAATCAGTG5290.10860995680239147No Hit
CCATAGGTTAATGAGGCGAACCGGG5290.10860995680239147No Hit
GCAGAGTACATGGGGGTTAAGCGAC5210.10696746218156136No Hit
CCCAGTGTGTTTCGACACACTATCA5180.10635152669875006No Hit
CAACGCAGAGTACTTTTTTTTTTTT5140.10553027938833501No Hit
ATGAAGGACGTGCTAATCTGCGATA5120.10511965573312748No Hit
CTACTCATCGAGCTCACAGCATGTG5070.10409309659510865No Hit
GTATCAACGCAGAGTACATGGGCGA5050.10368247293990113No Hit
CGTCTGGAAAGGCGCGCGATACAGG5010.10286122562948607No Hit
CACTAACACACACACTGATTCAGGC5010.10286122562948607No Hit
AACCCAGTGTGTTTCGACACACTAT4970.102039978319071No Hit
CGTGGTATCCTGTCTGAATATGGGG4970.102039978319071No Hit
CATGGGGGTTAAGCGACTAAGCGTA4890.10039748369824089No Hit
GTACACGGTGGATGCCCTGGCAGTC4880.10019217187063713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC250.005991315519.01911416
TGGTTCG250.006019980619.0034075
GCGCAGA654.8567017E-1018.9994851
ATATCAA552.8281647E-818.9994831
GTCATTA402.7567692E-416.6245481
GGTATCA22700.014.8982731
CCGCAGA456.7518424E-414.7773761
AGTATCA456.7518424E-414.7773761
CTATCAA456.7518424E-414.7773761
GATCAAC851.7074854E-814.5290171
GGTGATC400.00525148414.25844318
AGACCCG400.00526589714.2525555
GACCCGA400.00526589714.2525556
TGTAGGC400.00531296914.23345410
AAATCGC1152.5465852E-1114.05905717
ATCGCCG1257.2759576E-1213.692348519
GAAATCG1205.0931703E-1113.47187116
AATCGCC1301.4551915E-1113.1616418
ACAGGGT5900.012.562873
AGGGTGA6200.012.1070095
Produced by FastQC (version 0.11.5)