FastQCFastQC Report
Thu 26 May 2016
SRR1512280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487064
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT195294.0095346812739185No Hit
GGTATCAACGCAGAGTACTTTTTTT130642.682193715815581No Hit
TATCAACGCAGAGTACTTTTTTTTT126542.598015866498037No Hit
GTACTGGTTCACTATCGGTCAGTCA95261.9558004697534617No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64371.3215922342854327No Hit
GGATACCACGTGTCCCGCCCTACTC60081.2335134602434177No Hit
ATCATTAACTGAATCCATAGGTTAA58421.1994316968611929No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52691.0817880196442358No Hit
GTACATGGGGGTTAAGCGACTAAGC46630.9573690521163544No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43860.9004976758701115No Hit
GGTTAATGAGGCGAACCGGGGGAAC39630.8136507727937191No Hit
GTATTTAGCCTTGGAGGATGGTCCC38340.7871655470328335No Hit
ATACAGGGTGACAGCCCCGTACACA36370.7467191169948918No Hit
GTGGATGCCCTGGCAGTCAGAGGCG34760.7136639127506857No Hit
TATTCAGACAGGATACCACGTGTCC33570.6892318052658377No Hit
CCCATATTCAGACAGGATACCACGT33420.6861521278517813No Hit
AGCGTACACGGTGGATGCCCTGGCA32660.6705484289538952No Hit
CTATCGGTCAGTCAGGAGTATTTAG32100.6590509666080844No Hit
GTTCACTATCGGTCAGTCAGGAGTA31540.6475535042622735No Hit
GTATCAACGCAGAGTACATGGGGGT30250.6210682785013879No Hit
GTCAGGAGTATTTAGCCTTGGAGGA28110.5771315473941824No Hit
GGTATCAACGCAGAGTACATGGGGG26980.5539313108749568No Hit
GCAGAGTACTTTTTTTTTTTTTTTT26970.5537259990473531No Hit
GCCTTGGAGGATGGTCCCCCCATAT26290.5397647947702972No Hit
ACCCTGTATCGCGCGCCTTTCCAGA26180.5375063646666557No Hit
TCTAAGTACCCCGAGGAAAAGAAAT26140.5366851173562406No Hit
CGGTACTGGTTCACTATCGGTCAGT24480.5026033539740157No Hit
GTTAATGATAGTGTGTCGAAACACA22680.46564722500533806No Hit
ACTTAGATGTTTCAGTTCCCCCGGT22120.45414976265952733No Hit
TCACGGTACTGGTTCACTATCGGTC21970.45107008524547076No Hit
GGTTCACTATCGGTCAGTCAGGAGT21890.44942759062464066No Hit
GCCCAGAGCCTGAATCAGTGTGTGT21030.4317707734507169No Hit
GAGTACATGGGGGTTAAGCGACTAA20730.425611418622604No Hit
GTTATAACCGGCGATTTCCGAATGG20440.4196573756220948No Hit
GTGGTATCAACGCAGAGTACATGGG19960.4098024078971142No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG19920.4089811605866991No Hit
TATCAACGCAGAGTACATGGGGGTT19150.3931721498612092No Hit
GATATGAACCGTTATAACCGGCGAT19130.3927615262060017No Hit
ACGCAGAGTACATGGGGGTTAAGCG19030.39070840792996403No Hit
GTGCTAATCTGCGATAAGCGTCGGT18670.3833171821362285No Hit
CATCTAAGTACCCCGAGGAAAAGAA18600.38187999934300215No Hit
GGTGATATGAACCGTTATAACCGGC18250.3746940853768704No Hit
GGAGTATTTAGCCTTGGAGGATGGT18140.37243565527322897No Hit
GTGGTATCAACGCAGAGTACTTTTT18100.3716144079628139No Hit
CACTATCATTAACTGAATCCATAGG17450.35826913916856923No Hit
ATCAACGCAGAGTACTTTTTTTTTT16890.3467716768227584No Hit
GCCTGAATCAGTGTGTGTGTTAGTG16620.3412282574774568No Hit
ATCCATAGGTTAATGAGGCGAACCG16110.33075735426966474No Hit
GAATCAGTGTGTGTGTTAGTGGAAG15350.31515365537177864No Hit
CTATCATTAACTGAATCCATAGGTT14990.3077624295780431No Hit
GTACTTAGATGTTTCAGTTCCCCCG14960.30714649409523187No Hit
GCGTACACGGTGGATGCCCTGGCAG14540.29852339733587374No Hit
CACACACACTGATTCAGGCTCTGGG14490.29749683819785494No Hit
CCGTTATAACCGGCGATTTCCGAAT14460.29688090271504364No Hit
TCATTAACCTATGGATTCAGTTAAT14310.2938012253009872No Hit
TCGGTAAGGTGATATGAACCGTTAT13850.284356881231214No Hit
GTCCCGCCCTACTCATCGAGCTCAC13600.2792240855411199No Hit
GCCCTACTCATCGAGCTCACAGCAT13360.2742966016786295No Hit
CCCCAGTAGCGGCGAGCGAACGGGG12330.25314948343544175No Hit
TTTGTGTACGGGGCTGTCACCCTGT11690.2400095264688008No Hit
AGCTCACAGCATGTGCATTTTTGTG11450.23508204260631044No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG11400.23405548346829164No Hit
AGTCAGGAGTATTTAGCCTTGGAGG11170.22933331143340507No Hit
CATTAACTGAATCCATAGGTTAATG11170.22933331143340507No Hit
GTCCCCCCATATTCAGACAGGATAC10970.22522707488132976No Hit
GGGAAACCCAGTGTGTTTCGACACA10850.2227633329500846No Hit
GTACATGGGCATCTAAGTACCCCGA10370.21290836522510387No Hit
CCTATGGATTCAGTTAATGATAGTG10360.21270305339750012No Hit
GGGTACTTAGATGTTTCAGTTCCCC10340.21229242974229262No Hit
CAGTAGCGGCGAGCGAACGGGGAGC10320.21188180608708507No Hit
GTGTTTCGACACACTATCATTAACT10260.21064993512146246No Hit
GATTCAGGCTCTGGGCTGCTCCCCG10060.2065436985693872No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA9700.19915247277565168No Hit
GGTATCAACGCAGAGTACATGGGGA9640.19792060181002907No Hit
GGGTGACAGCCCCGTACACAAAAAT9630.19771528998242532No Hit
TAAGTACCCCGAGGAAAAGAAATCA9590.19689404267201027No Hit
CCCTGGCAGTCAGAGGCGATGAAGG9580.1966887308444065No Hit
GGGGAAACCCAGTGTGTTTCGACAC9500.19504623622357636No Hit
ATTTAGCCTTGGAGGATGGTCCCCC9460.1942249889131613No Hit
TTCCAGACGCTTCCACTAACACACA8760.1798531609808978No Hit
AGGTTAATGAGGCGAACCGGGGGAA8740.17944253732569024No Hit
GGAGGATGGTCCCCCCATATTCAGA8660.17780004270486013No Hit
CACTGATTCAGGCTCTGGGCTGCTC8620.17697879539444508No Hit
GTGCATTTTTGTGTACGGGGCTGTC8550.17554161260121873No Hit
CCACGTGTCCCGCCCTACTCATCGA8430.17307787066997354No Hit
GGGGTTAAGCGACTAAGCGTACACG8420.1728725588423698No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA8370.17184599970435097No Hit
AAGTACCCCGAGGAAAAGAAATCAA8300.1704088169111246No Hit
GTGGTATCCTGTCTGAATATGGGGG8150.16732913949706815No Hit
GTATCGCGCGCCTTTCCAGACGCTT8040.16507070939342675No Hit
GGGTTTCCCCATTCGGAAATCGCCG7990.16404415025540792No Hit
TCCCCCAGTAGCGGCGAGCGAACGG7960.16342821477259661No Hit
ATGATAGTGTGTCGAAACACACTGG7840.16096447284135146No Hit
GGGCTGTCACCCTGTATCGCGCGCC7810.16034853735854016No Hit
GGTATCAACGCAGAGTACATGGGCA7790.15993791370333263No Hit
GTCAGAGGCGATGAAGGACGTGCTA7780.15973260187572885No Hit
ATTCAGACAGGATACCACGTGTCCC7660.1572688599444837No Hit
GTACGGGGCTGTCACCCTGTATCGC7570.15542105349604982No Hit
TAATGATAGTGTGTCGAAACACACT7480.15357324704761594No Hit
CGGTGGATGCCCTGGCAGTCAGAGG7460.1531626233924084No Hit
AACACACACACTGATTCAGGCTCTG7400.1519307524267858No Hit
GCATGTGCATTTTTGTGTACGGGGC7220.14823513952991804No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC6860.14084391373618252No Hit
GTATCAACGCAGAGTACATGGGCAT6840.140433290080975No Hit
GTACATGGGATAGGTTAATGAGGCG6770.13899610728774864No Hit
TATTTAGCCTTGGAGGATGGTCCCC6640.13632705352889968No Hit
ACCCAGTGTGTTTCGACACACTATC6590.13530049439088088No Hit
CATTAACCTATGGATTCAGTTAATG6430.1320155051492206No Hit
CTAACACACACACTGATTCAGGCTC6340.13016769870078673No Hit
ACATGGGGGTTAAGCGACTAAGCGT6320.1297570750455792No Hit
GGTATCAACGCAGAGTACATGGGAT6260.12852520407995663No Hit
CACGTGTCCCGCCCTACTCATCGAG6260.12852520407995663No Hit
GAGTAGGGCGGGACACGTGGTATCC6240.1281145804247491No Hit
CAACGCAGAGTACTTTTTTTTTTTT6180.12688270945912652No Hit
GACTAAGCGTACACGGTGGATGCCC6170.12667739763152275No Hit
GTATCAACGCAGAGTACATGGGGAA6150.12626677397631522No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT6110.12544552666590017No Hit
GCGATAAGCGTCGGTAAGGTGATAT6100.1252402148382964No Hit
GAATATGGGGGGACCATCCTCCAAG6090.12503490301069264No Hit
AATCTGCGATAAGCGTCGGTAAGGT6080.12482959118308888No Hit
CCCTCACGGTACTGGTTCACTATCG5970.12257116107944746No Hit
GATGAAGGACGTGCTAATCTGCGAT5890.12092866645861734No Hit
GTATCAACGCAGAGTACATGGGGAG5880.12072335463101358No Hit
ATGAAGGACGTGCTAATCTGCGATA5850.12010741914820228No Hit
GGATGGTCCCCCCATATTCAGACAG5850.12010741914820228No Hit
CTACTGGGGGAATCTCGGTTGATTT5830.11969679549299475No Hit
GCATTTTTGTGTACGGGGCTGTCAC5820.119491483665391No Hit
CTGTCACCCTGTATCGCGCGCCTTT5810.11928617183778722No Hit
CCCAGTGTGTTTCGACACACTATCA5790.1188755481825797No Hit
GTACATGGGGAGCGAACGGGGAGCA5750.11805430087216465No Hit
GAGCAGCCCAGAGCCTGAATCAGTG5730.11764367721695712No Hit
GTCTGAATATGGGGGGACCATCCTC5720.11743836538935334No Hit
TCAACGCAGAGTACTTTTTTTTTTT5680.11661711807893829No Hit
CATGGGGGTTAAGCGACTAAGCGTA5640.11579587076852324No Hit
CACTAACACACACACTGATTCAGGC5590.11476931163050441No Hit
AACGCAGAGTACTTTTTTTTTTTTT5520.11333212883727806No Hit
CTACTCATCGAGCTCACAGCATGTG5490.11271619335446675No Hit
CGTGGTATCCTGTCTGAATATGGGG5420.1112790105612404No Hit
TTTCAGTTCCCCCGGTTCGCCTCAT5400.11086838690603287No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT5190.10655683852635384No Hit
GTCACCCTGTATCGCGCGCCTTTCC5170.10614621487114631No Hit
CCATAGGTTAATGAGGCGAACCGGG5160.10594090304354253No Hit
GTACATGGGCGAGCGAACGGGGAGC5160.10594090304354253No Hit
CAGTTAATGATAGTGTGTCGAAACA5120.10511965573312748No Hit
CAGGAGTATTTAGCCTTGGAGGATG5070.10409309659510865No Hit
GGTTAAGCGACTAAGCGTACACGGT5070.10409309659510865No Hit
GCAGAGTACATGGGGGTTAAGCGAC4990.10245060197427855No Hit
AACCCAGTGTGTTTCGACACACTAT4960.10183466649146725No Hit
GCTGTCACCCTGTATCGCGCGCCTT4950.10162935466386347No Hit
GTGTGTTTCGACACACTATCATTAA4950.10162935466386347No Hit
AGATTCCCCCAGTAGCGGCGAGCGA4880.10019217187063713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGAAC250.006012153319.0077088
GGTGATC552.8408977E-818.99209218
TAACGCC250.006040766418.99209219
CGGTGAT607.689414E-817.40941817
GTGATCT607.689414E-817.40941819
CCGGTGA607.689414E-817.40941816
AGACCCG702.4781912E-816.2923225
GACCCGA702.4781912E-816.2923226
TAGACCC702.4781912E-816.2923224
TATAGAC702.4812834E-816.2906482
AAACCCG702.5032932E-816.27893612
CAACTTA350.002173357216.27893614
TATGCGT350.002173357216.27893610
CGAAACC702.5032932E-816.27893610
AACTTAA350.00217484916.27726615
GTATAGA807.557901E-915.4548821
ACCCGAA755.7882062E-815.2061677
CCCGAAA755.7882062E-815.2061678
ATAGACC755.7882062E-815.2061673
ACCCGGT704.438698E-714.922358514
Produced by FastQC (version 0.11.5)