FastQCFastQC Report
Thu 26 May 2016
SRR1512279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874335
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63590.3392669933603118No Hit
GTCCTACAGTGGACATTTCTAAATT56660.3022938802295214No Hit
GTCCTAAAGTGTGTATTTCTCATTT55060.2937575193335236No Hit
CTGTAGGACGTGGAATATGGCAAGA52290.27897894453232747No Hit
CTTTAGGACGTGAAATATGGCGAGG47460.25320980507753416No Hit
GGTATCAACGCAGAGTACTTTTTTT42100.2246129960759416No Hit
TATCAACGCAGAGTACTTTTTTTTT37220.19857709534314835No Hit
GTCCTACAGTGTGCATTTCTCATTT35870.19137454083715025No Hit
CTGAAGGACCTGGAATATGGCGAGA24950.13311387772196537No Hit
CTGTAGGACCTGGAATATGGCGAGA24310.12969933336356626No Hit
ATTTAGAAATGTCCACTGTAGGACG22850.12190990404596831No Hit
GTCCTTCAGTGTGCATTTCTCATTT21390.11412047472837032No Hit
TTTCTAAATTTTCCACCTTTTTCAG19690.10505059127637267No Hit
GAATATGGCAAGAAAACTGAAAATC18840.10051564955037386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10550.014.4072584
GGTATCA12250.013.5711221
GTCGAGG604.0694728E-412.67521819
GGCGAGG13250.011.90992119
ATAGGAC2500.011.4000473
CACCTTT6600.011.38285914
AGGACCT19500.011.3507225
GGACCTG18600.011.3381496
ACACCGT600.00587856411.0827876
CGCCCTA1657.8216544E-1110.95177516
AGGACGT31000.010.9091395
GACGTGG15300.010.8654777
AGGGCCG700.001492259610.85660655
TGTAGGA33100.010.8202882
GGACGTG31000.010.755926
TGGCGAG31500.010.73856918
GGACTGT3100.010.7252766
AAATGTC8550.010.666147
TTAGGAC18550.010.6523363
GTCCTAT2950.010.6268791
Produced by FastQC (version 0.11.5)