##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512279_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1874335 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.075801817711348 31.0 30.0 34.0 16.0 34.0 2 29.96315599932776 31.0 30.0 34.0 23.0 34.0 3 30.618329167411375 33.0 31.0 34.0 26.0 34.0 4 34.2755622660837 37.0 35.0 37.0 31.0 37.0 5 34.45446144899391 37.0 35.0 37.0 32.0 37.0 6 34.6065890035666 37.0 35.0 37.0 32.0 37.0 7 34.55336586042517 37.0 35.0 37.0 32.0 37.0 8 34.55362195125205 37.0 35.0 37.0 32.0 37.0 9 36.15016525861172 39.0 37.0 39.0 32.0 39.0 10 36.07680430659407 39.0 37.0 39.0 32.0 39.0 11 36.101217231711516 39.0 37.0 39.0 32.0 39.0 12 36.04432078577202 39.0 37.0 39.0 32.0 39.0 13 36.01939888013616 39.0 37.0 39.0 32.0 39.0 14 37.260443837414336 40.0 38.0 41.0 32.0 41.0 15 37.19248053309574 40.0 38.0 41.0 31.0 41.0 16 37.1194807758485 40.0 38.0 41.0 31.0 41.0 17 37.084933589779844 40.0 38.0 41.0 31.0 41.0 18 36.871033726628376 40.0 38.0 41.0 31.0 41.0 19 36.981924789325284 40.0 38.0 41.0 31.0 41.0 20 36.91841533130417 40.0 38.0 41.0 31.0 41.0 21 36.85876804306594 40.0 38.0 41.0 31.0 41.0 22 36.785468979664785 40.0 38.0 41.0 31.0 41.0 23 36.66881747393075 40.0 38.0 41.0 30.0 41.0 24 36.55486345823986 40.0 37.0 41.0 30.0 41.0 25 36.43983652868884 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.05989956832574 1101 2 0.1257540356595115 1101 3 -0.22497244023225704 1101 4 -0.3450831551418503 1101 5 -0.4843486665463814 1101 6 -0.6370587055721444 1101 7 -0.6488172363901938 1101 8 -0.9075544699323146 1101 9 -0.8077611620561385 1101 10 -0.9807831306447028 1101 11 -1.0349915569829093 1101 12 -1.0761233104324717 1101 13 -1.1298001052900943 1101 14 -1.3974910530578626 1101 15 -1.5296985478660687 1101 16 -1.6073849810716823 1101 17 -1.6347089036861604 1101 18 -1.592233457643438 1101 19 -1.6064296433563712 1101 20 -1.7652115319698964 1101 21 -1.9128983484986009 1101 22 -1.828898448834245 1101 23 -1.799418776869416 1101 24 -1.928265605836664 1101 25 -2.04073221748596 1102 1 0.7626688417539711 1102 2 0.10149872625311573 1102 3 -0.4394814258707527 1102 4 -0.37292534267961486 1102 5 -0.6008939050928532 1102 6 -0.767194650797208 1102 7 -0.7828469158988156 1102 8 -0.8273037954847524 1102 9 -1.0728343178347686 1102 10 -0.9693296055193841 1102 11 -1.1044577481126368 1102 12 -1.282084523814838 1102 13 -1.0563463741100705 1102 14 -1.4062280267757146 1102 15 -1.4490379899157304 1102 16 -1.5125095106119133 1102 17 -1.5478822859977655 1102 18 -1.6220538339094972 1102 19 -1.4610930301070582 1102 20 -1.5624409865990643 1102 21 -1.5780562792514985 1102 22 -1.8626259147811624 1102 23 -1.802560475090857 1102 24 -1.701024156788776 1102 25 -1.9718075559180903 1103 1 0.7796735970660755 1103 2 -0.020320331111598477 1103 3 -0.4504686045862165 1103 4 -0.730169669411687 1103 5 -0.872074907921963 1103 6 -0.8538117578820206 1103 7 -0.9295621389169355 1103 8 -1.028264488917607 1103 9 -1.2748730826574928 1103 10 -1.3010776341716408 1103 11 -1.2004374026000875 1103 12 -1.2050930111440366 1103 13 -1.2655674660260416 1103 14 -1.4927999884924361 1103 15 -1.5083439551236992 1103 16 -1.6986974357308 1103 17 -1.6829957513562164 1103 18 -1.5592746850501698 1103 19 -1.7564544940208577 1103 20 -1.6950564082568036 1103 21 -1.8118021409260905 1103 22 -1.8548789696177366 1103 23 -1.9758584947140463 1103 24 -2.202170247199902 1103 25 -2.2150375565200235 1104 1 1.088785444315743 1104 2 0.3476328087721008 1104 3 -0.2825879727708127 1104 4 -0.25075485217467275 1104 5 -0.4864795598121461 1104 6 -0.49244036018237836 1104 7 -0.5862489467136811 1104 8 -0.6001194159119336 1104 9 -0.8061328748010297 1104 10 -0.7130809894469579 1104 11 -0.6814255027465279 1104 12 -0.7557767195762963 1104 13 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16 1.0655590980306897 1201 17 1.0447192904182288 1201 18 1.23278370997852 1201 19 1.1431809830403594 1201 20 1.190732549017305 1201 21 1.163341914019945 1201 22 1.1288512953006489 1201 23 1.25286032311368 1201 24 1.2807970765595016 1201 25 1.1965962555098244 1202 1 2.5163918068381754 1202 2 1.7647381514319136 1202 3 1.2881201638229136 1202 4 1.123163467753784 1202 5 0.9046900878849513 1202 6 0.8527582961131799 1202 7 0.931226455883639 1202 8 0.7751794340472742 1202 9 0.8822994518803071 1202 10 0.9587325734229992 1202 11 1.0734283303075571 1202 12 1.0482830867829591 1202 13 1.0312519663622552 1202 14 1.167881498656115 1202 15 1.2432707515808303 1202 16 1.2164229868147771 1202 17 1.2739452070218675 1202 18 1.291067382657161 1202 19 1.3734785636966294 1202 20 1.1593892949314153 1202 21 1.215068344147987 1202 22 1.3073332153489403 1202 23 1.3321937055537205 1202 24 1.2934824768360542 1202 25 1.245505338034846 1203 1 2.4027771183419198 1203 2 1.634111960187532 1203 3 1.1015220806159576 1203 4 0.7774726988050915 1203 5 0.619524838038096 1203 6 0.5786446672114778 1203 7 0.6263943920265405 1203 8 0.607436590752144 1203 9 0.6741453352382081 1203 10 0.7065689680441523 1203 11 0.7857470586235351 1203 12 0.7180090987988095 1203 13 0.6444657002995555 1203 14 0.8652368472989664 1203 15 0.9686372035806983 1203 16 1.0499286315897862 1203 17 0.8995093647259225 1203 18 1.0446982173561494 1203 19 0.9587229811044011 1203 20 0.8218711197934425 1203 21 0.8765501277917096 1203 22 0.9221868690668984 1203 23 0.910063458570832 1203 24 0.9802995855571339 1203 25 0.9514969152885939 1204 1 2.6060550784962473 1204 2 1.7905697183432885 1204 3 1.2740693316072829 1204 4 0.9624979854292093 1204 5 0.8694522368547979 1204 6 0.7698570467503885 1204 7 0.7921137861111163 1204 8 0.7494993743818767 1204 9 0.8179660712067758 1204 10 0.8595734525891316 1204 11 0.9888451552011759 1204 12 0.8990886282093129 1204 13 0.9469079104267806 1204 14 1.171125372029536 1204 15 1.1593870767795522 1204 16 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-0.14625650962813808 2315 25 -0.018081948061826836 2316 1 1.1903084272347542 2316 2 0.4426403432142223 2316 3 -0.09661092893053791 2316 4 -0.32932576224148136 2316 5 -0.44777298113390884 2316 6 -0.5633003141408324 2316 7 -0.4816028144426241 2316 8 -0.4748421653731967 2316 9 -0.6455789050170324 2316 10 -0.7674387344506144 2316 11 -0.7213379035449847 2316 12 -0.729338544135409 2316 13 -0.7008807815613309 2316 14 -0.8260799276991264 2316 15 -0.8472816719748408 2316 16 -0.7210091814387667 2316 17 -0.7794743387116085 2316 18 -0.5706824301982039 2316 19 -0.733717195123738 2316 20 -0.6621042329960858 2316 21 -0.624574335984633 2316 22 -0.7022897544314048 2316 23 -0.6875402097253982 2316 24 -0.7528258750808376 2316 25 -0.731209177641702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49847.0 3 6.0 4 408.0 5 1904.0 6 2254.0 7 2225.0 8 2123.0 9 2000.0 10 2027.0 11 1974.0 12 2034.0 13 1994.0 14 2164.0 15 2043.0 16 2098.0 17 2397.0 18 2581.0 19 3182.0 20 3752.0 21 4657.0 22 5756.0 23 6808.0 24 8082.0 25 9439.0 26 10848.0 27 12228.0 28 13865.0 29 16884.0 30 21539.0 31 28088.0 32 36559.0 33 50358.0 34 70541.0 35 100917.0 36 174820.0 37 295700.0 38 920233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.818940527461116 19.617345777982393 11.931606178784318 24.632107515772176 2 14.250980908337441 20.251737490576165 42.962527776573886 22.534753824512517 3 18.697158563644127 25.413767378336928 29.815869101480175 26.07320495653877 4 11.837589137505338 17.116113406773113 39.232756581700414 31.813540874021136 5 12.410782502990859 38.46935412417577 36.364784262194036 12.755079110639327 6 30.65996515183766 37.88373891474394 18.715538237247745 12.74075769617066 7 27.346542997623246 31.624379642474885 23.0741141516743 17.954963208227564 8 24.1486326585331 38.11232541972318 21.01053596017881 16.72850596156491 9 26.674639932690802 14.845625838088855 20.594026148104845 37.885708081115496 10 15.066091921753838 28.716123177652754 35.36034121067546 20.857443689917954 11 33.82669829308275 21.823350242947566 26.087995956667886 18.261955507301796 12 23.922900432668907 24.273207151605263 32.7457094112173 19.05818300450853 13 29.5756498817327 22.336204341923537 25.814311252135603 22.273834524208155 14 20.255829327308785 23.060902270116234 28.067363254967148 28.61590514760783 15 22.309629792211478 32.782513965433715 23.404273543165036 21.503582699189778 16 21.085573854439065 27.86204096412131 29.635031726357358 21.41735345508227 17 20.949732342995272 29.276297461437245 28.725071896657393 21.048898298910085 18 20.76599345033563 26.917721109928284 31.820247981339172 20.496037458396916 19 23.474828479804607 26.40879474042129 28.15137475246229 21.965002027311815 20 23.698207328911526 27.283709871237292 28.95690875423899 20.06117404561219 21 22.39555327021078 26.670543675682495 27.59386335039859 23.340039703708136 22 23.12007215571614 28.89616288041515 27.75467697706876 20.22908798679995 23 21.37855528373274 28.42125024469402 28.97551562244275 21.224678849130495 24 23.099831740948513 26.58305642593791 29.653208619274686 20.663903213838893 25 22.176312154659144 27.734114119871872 29.175755699647116 20.91381802582187 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 976.0 1 976.0 2 1361.5 3 1747.0 4 1747.0 5 1747.0 6 2813.0 7 3879.0 8 3879.0 9 3879.0 10 4512.5 11 5146.0 12 5146.0 13 5146.0 14 7110.5 15 9075.0 16 9075.0 17 9075.0 18 16089.5 19 23104.0 20 23104.0 21 23104.0 22 37356.0 23 51608.0 24 51608.0 25 51608.0 26 75923.0 27 100238.0 28 100238.0 29 100238.0 30 131257.0 31 162276.0 32 162276.0 33 162276.0 34 179163.5 35 196051.0 36 196051.0 37 196051.0 38 206249.5 39 216448.0 40 216448.0 41 216448.0 42 223878.0 43 231308.0 44 231308.0 45 231308.0 46 236867.5 47 242427.0 48 242427.0 49 242427.0 50 230317.5 51 218208.0 52 218208.0 53 218208.0 54 189310.5 55 160413.0 56 160413.0 57 160413.0 58 136951.0 59 113489.0 60 113489.0 61 113489.0 62 92478.5 63 71468.0 64 71468.0 65 71468.0 66 54616.5 67 37765.0 68 37765.0 69 37765.0 70 27735.0 71 17705.0 72 17705.0 73 17705.0 74 12424.0 75 7143.0 76 7143.0 77 7143.0 78 4832.0 79 2521.0 80 2521.0 81 2521.0 82 1702.5 83 884.0 84 884.0 85 884.0 86 576.5 87 269.0 88 269.0 89 269.0 90 187.0 91 105.0 92 105.0 93 105.0 94 78.0 95 51.0 96 51.0 97 51.0 98 41.0 99 31.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3377197779479122 2 0.28831558926232503 3 0.3080025715787199 4 0.29839916557072244 5 0.2831404204691264 6 0.24056532050033747 7 0.3554327268071076 8 0.3084827418791198 9 0.25363662312233404 10 0.2287744720127405 11 0.24488685320393633 12 0.24248600170193696 13 0.25827826935953285 14 0.2481413407955355 15 0.3377197779479122 16 0.3965673158746969 17 0.4143869692450923 18 0.11620121269676979 19 0.12793870892876674 20 0.19244158594914998 21 0.18550579272115175 22 0.15082682658116078 23 0.24824804530673544 24 0.34049409523911145 25 0.38189544558470073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1874335.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.08534661253502 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6662615091639 53.96943198383024 2 12.088200445805725 15.977058327657431 3 3.281387123906908 6.505548163598923 4 1.2043728937271694 3.1836560053080722 5 0.5380691066837597 1.7779241708346671 6 0.3187341097961288 1.263819247386898 7 0.1945918369985606 0.9001768297213855 8 0.13126023935386588 0.6939502731315629 9 0.08698869473107135 0.5173810238407364 >10 0.4300045771621903 5.112639093300541 >50 0.029681709987528268 1.401731813830764 >100 0.02526609324573203 3.517349193245841 >500 0.0026717931474746015 1.239763485932008 >1k 0.0021860125752064903 2.721797442033628 >5k 3.23853714845406E-4 1.217772946347265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6359 0.3392669933603118 No Hit GTCCTACAGTGGACATTTCTAAATT 5666 0.3022938802295214 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5506 0.2937575193335236 No Hit CTGTAGGACGTGGAATATGGCAAGA 5229 0.27897894453232747 No Hit CTTTAGGACGTGAAATATGGCGAGG 4746 0.25320980507753416 No Hit GGTATCAACGCAGAGTACTTTTTTT 4210 0.2246129960759416 No Hit TATCAACGCAGAGTACTTTTTTTTT 3722 0.19857709534314835 No Hit GTCCTACAGTGTGCATTTCTCATTT 3587 0.19137454083715025 No Hit CTGAAGGACCTGGAATATGGCGAGA 2495 0.13311387772196537 No Hit CTGTAGGACCTGGAATATGGCGAGA 2431 0.12969933336356626 No Hit ATTTAGAAATGTCCACTGTAGGACG 2285 0.12190990404596831 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2139 0.11412047472837032 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1969 0.10505059127637267 No Hit GAATATGGCAAGAAAACTGAAAATC 1884 0.10051564955037386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.0670451119997226E-4 0.0 11 0.0 0.0 0.0 1.0670451119997226E-4 0.0 12 0.0 0.0 0.0 1.0670451119997226E-4 0.0 13 0.0 0.0 0.0 1.0670451119997226E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1055 0.0 14.407258 4 GGTATCA 1225 0.0 13.571122 1 GTCGAGG 60 4.0694728E-4 12.675218 19 GGCGAGG 1325 0.0 11.909921 19 ATAGGAC 250 0.0 11.400047 3 CACCTTT 660 0.0 11.382859 14 AGGACCT 1950 0.0 11.350722 5 GGACCTG 1860 0.0 11.338149 6 ACACCGT 60 0.005878564 11.082787 6 CGCCCTA 165 7.8216544E-11 10.951775 16 AGGACGT 3100 0.0 10.909139 5 GACGTGG 1530 0.0 10.865477 7 AGGGCCG 70 0.0014922596 10.8566065 5 TGTAGGA 3310 0.0 10.820288 2 GGACGTG 3100 0.0 10.75592 6 TGGCGAG 3150 0.0 10.738569 18 GGACTGT 310 0.0 10.725276 6 AAATGTC 855 0.0 10.66614 7 TTAGGAC 1855 0.0 10.652336 3 GTCCTAT 295 0.0 10.626879 1 >>END_MODULE