FastQCFastQC Report
Thu 26 May 2016
SRR1512279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874335
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73260.39085862452549835No Hit
GTCCTACAGTGGACATTTCTAAATT56150.2995729151939221No Hit
GTCCTAAAGTGTGTATTTCTCATTT55960.29855922233752236No Hit
CTGTAGGACGTGGAATATGGCAAGA55540.29631842760232296No Hit
CTTTAGGACGTGAAATATGGCGAGG51760.27615127498552816No Hit
GGTATCAACGCAGAGTACTTTTTTT48330.2578514513147329No Hit
TATCAACGCAGAGTACTTTTTTTTT45670.24365975132513662No Hit
GTCCTACAGTGTGCATTTCTCATTT35680.1903608479807505No Hit
CTGAAGGACCTGGAATATGGCGAGA28140.15013324725836097No Hit
CTGTAGGACCTGGAATATGGCGAGA26540.1415968863623632No Hit
ATTTAGAAATGTCCACTGTAGGACG23440.1250576871263675No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22880.12206996081276826No Hit
TTTCTAAATTTTCCACCTTTTTCAG22170.11828195066516925No Hit
GTCCTTCAGTGTGCATTTCTCATTT21110.11262661157157071No Hit
GAATATGGCAAGAAAACTGAAAATC19240.1026497397743733No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19110.1019561604515735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.013.9947411
TCCAACG1301.4551915E-1113.14311518
CGGGACC604.0812875E-412.6708684
TAGGACC11150.012.3583584
CCAACGT701.0994753E-412.20432219
AGTCGTC1750.011.9516078
GGCGAGG14400.011.86531319
AAGTCGT1850.011.8194657
GGACCGA657.975616E-411.7008736
ATACGGC907.5241333E-611.60256815
AGGACCT20500.011.4513625
GCGACTA1001.9491254E-611.390716
CTCGAAC1501.7826096E-1011.390718
CGACTAA1001.9491254E-611.390717
AATACGG855.359674E-511.16914114
TGTAGGA30750.011.0941442
CCACCTT7250.011.00437513
GTCTTAT1303.220339E-810.9722071
CACCTTT7850.010.88457714
GCGCCAC700.001494502510.85465613
Produced by FastQC (version 0.11.5)