FastQCFastQC Report
Thu 26 May 2016
SRR1512278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682110
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60150.3575866025408564No Hit
GTCCTACAGTGGACATTTCTAAATT59270.3523550778486544No Hit
GTCCTAAAGTGTGTATTTCTCATTT58700.34896647662756897No Hit
CTGTAGGACGTGGAATATGGCAAGA55800.331726224800994No Hit
CTTTAGGACGTGAAATATGGCGAGG51390.30550915219575414No Hit
GGTATCAACGCAGAGTACTTTTTTT39380.234110729976042No Hit
TATCAACGCAGAGTACTTTTTTTTT37680.2240043754570153No Hit
GTCCTACAGTGTGCATTTCTCATTT29400.17478048403493232No Hit
CTGAAGGACCTGGAATATGGCGAGA27150.16140442658327933No Hit
GTCCTTCAGTGTGCATTTCTCATTT23800.14148896326637378No Hit
ATTTAGAAATGTCCACTGTAGGACG22420.1332849813626933No Hit
GAATATGGCAAGAAAACTGAAAATC21450.1275184143724251No Hit
TTTCTAAATTTTCCACCTTTTTCAG20820.12377311828596228No Hit
CTGTAGGACCTGGAATATGGCGAGA20650.1227624828340596No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16830.10005290973836432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAT350.002168401816.287847
GGTATCA12300.014.8303181
TAGGACC9050.013.0182744
CGCTCTG550.003065614712.0924875
GTATAGT1201.0002623E-811.875841
AGGACCT18450.011.380895
GGCGAGG14350.011.32779119
CCACCTT7400.011.043632513
AAATGTC8250.010.9408237
TCGAACT700.001483578910.86407919
GTCCTAA18600.010.8287241
CGACTAA803.734326E-410.69880817
CACCTTT7200.010.69752814
TCCAACG1252.2367021E-710.64107118
GTCCTAT2150.010.6054011
GGACCTG18350.010.5626866
AATGTCC8200.010.5223598
GTGTAGG3350.010.493281
ATGTCCA8350.010.4496949
TAGGACA8850.010.4137894
Produced by FastQC (version 0.11.5)