FastQCFastQC Report
Thu 26 May 2016
SRR1512278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682110
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69060.41055579004940224No Hit
CTGTAGGACGTGGAATATGGCAAGA59650.35461414532937796No Hit
GTCCTAAAGTGTGTATTTCTCATTT59440.35336571330055705No Hit
GTCCTACAGTGGACATTTCTAAATT59180.3518200355505883No Hit
CTTTAGGACGTGAAATATGGCGAGG56600.33648215633935946No Hit
GGTATCAACGCAGAGTACTTTTTTT49970.2970673737151554No Hit
TATCAACGCAGAGTACTTTTTTTTT45460.2702558096676198No Hit
GTCCTACAGTGTGCATTTCTCATTT30520.181438788188644No Hit
CTGAAGGACCTGGAATATGGCGAGA29970.17816908525601774No Hit
GTCCTTCAGTGTGCATTTCTCATTT24090.14321298844903127No Hit
ATTTAGAAATGTCCACTGTAGGACG23520.13982438722794585No Hit
CTGTAGGACCTGGAATATGGCGAGA22820.13566294713187604No Hit
GAATATGGCAAGAAAACTGAAAATC22540.13399837109344812No Hit
TTTCTAAATTTTCCACCTTTTTCAG21960.1305503207281331No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21500.12781566009357295No Hit
GGAATATGGCGAGAAAACTGAAAAT18560.11033761169007973No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17920.10653286645938731No Hit
ATTCCAGGTCCTTCAGTGTGCATTT16910.10052850289220087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.014.2258511
GTCGAGG1550.013.47743619
GGACGTT655.43485E-513.1585226
TAGGACC8300.012.3647374
TGTCGAG1850.012.31807618
AGGACCG802.8571481E-511.8788685
CCAACGA802.8807264E-511.86933219
GGCGAGG14050.011.82709319
TAGAAAT7650.011.8005274
AAATGTC7850.011.7437267
GTATTAA2050.011.59569551
ATGTCGA2000.011.3942217
TATGTCG1950.011.19911516
TGTAGGA32200.011.0957912
TCCAACG1201.2842247E-711.07771518
GACGTGG16500.011.0015357
GAAATGT8850.010.9530456
AGGACGT36200.010.9206845
CTGTAGG31850.010.8668241
AATGTCC8000.010.8107368
Produced by FastQC (version 0.11.5)