FastQCFastQC Report
Thu 26 May 2016
SRR1512277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436351
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71910.500643644902952No Hit
GGTATCAACGCAGAGTACTTTTTTT41540.28920507591807293No Hit
TATCAACGCAGAGTACTTTTTTTTT40500.28196450589027333No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20340.14160884073600394No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18280.12726694241170855No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14930.10394395241831557No Hit
GTCCTACAGTGGACATTTCTAAATT14660.10206418904571375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.014.292521
GTCTACG400.005272450414.2525981
TATGCCG400.00527367414.2520995
CTAGTGC751.475619E-512.6689753
TGGTATC2950.012.2395192
TACCGCC550.003064053812.0931137
CCTAGAC951.0372823E-612.0021873
TAAGTAG802.8616678E-511.8767495
GTTCTAA1604.5474735E-1111.2833061
CTAGACT1451.2132659E-911.139964
GTATTAG1201.2714554E-711.0853531
GTATCAA29800.011.0322111
GTAAGAC951.3571285E-511.0020053
TAGGACC3550.010.973834
GGACGTG8350.010.9237646
TAGGACG8850.010.6290564
GACGTGG4300.010.6065857
GTAGGAC8700.010.5938853
GTTAGCC909.495366E-510.557483
GTACTAG909.495366E-510.557481
Produced by FastQC (version 0.11.5)