FastQCFastQC Report
Thu 26 May 2016
SRR1512277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436351
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83580.5818911951187419No Hit
GGTATCAACGCAGAGTACTTTTTTT53890.3751868449981933No Hit
TATCAACGCAGAGTACTTTTTTTTT52600.3662057533290958No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26210.1824762888736806No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19760.13757083052819263No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17620.12267196527868188No Hit
CTGTAGGACGTGGAATATGGCAAGA15610.1086781712826461No Hit
GTCCTACAGTGGACATTTCTAAATT15380.10707689137265194No Hit
GTGGTATCAACGCAGAGTACATGGG14820.10317812289614448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3650.014.3187444
GGTATCA15350.013.8173651
GGACCGT500.001493018913.3075886
ATGTCGA500.00150640513.29183517
AGGACCG550.003061555312.0944365
GTGGTAT4550.011.9149491
GGCGAGG3450.011.83372419
TGGTATC4150.011.4483232
GCCTAAG752.0513214E-411.4132681
GTAGGAC9800.011.2477853
GGTCTAT951.3424618E-511.0128021
CCTACAC2250.010.9805973
GCACCGT700.001484202610.86333756
GGATAGT803.750474E-410.6935986
TAAGACT1605.4205884E-1010.6902454
GTCCTAA4400.010.5918581
GTCTCGC909.401462E-510.567841
TGGCGAG8750.010.52496418
TCTATAC1909.094947E-1210.5026963
GTGTAGA2450.010.4815721
Produced by FastQC (version 0.11.5)