FastQCFastQC Report
Thu 26 May 2016
SRR1512276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999793
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71020.3551367566543137No Hit
GGTATCAACGCAGAGTACTTTTTTT44670.22337311911782867No Hit
TATCAACGCAGAGTACTTTTTTTTT41750.2087716078614137No Hit
GTCCTACAGTGGACATTTCTAAATT32170.16086664969824377No Hit
GTCCTAAAGTGTGTATTTCTCATTT29110.14556506598432936No Hit
CTGTAGGACGTGGAATATGGCAAGA28720.14361486413843833No Hit
CTTTAGGACGTGAAATATGGCGAGG25660.12831328042452395No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20750.10376073923651098No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20590.10296065642794029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC901.364242E-1015.81822910
GGTATCA12750.013.4121021
CGTCTAC604.092054E-412.66698551
AAGGCGT1251.4133548E-912.1593896
GTATTAA2900.011.4658051
GTCCTAT2300.011.1524531
GTCCTAG2400.011.0836121
GCGCCAC1553.110472E-1011.03558513
GCGTGCG1159.003761E-710.7218879
TGCGCCA1659.367795E-1010.35921112
TAGGACC5450.010.2841454
GGCGAGG8800.010.26290419
GTATCAA27150.010.1125931
AGGACGT18700.010.1093715
GTCCTAC17250.010.0785141
GTCCTAA9750.010.0361491
CGCCCTA2400.09.90628916
GACGTGA9850.09.8370697
GGACGTG18550.09.832396
TTAGGAC11250.09.7960483
Produced by FastQC (version 0.11.5)