FastQCFastQC Report
Thu 26 May 2016
SRR1512276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999793
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82360.41184262571176117No Hit
GGTATCAACGCAGAGTACTTTTTTT54090.2704779944724279No Hit
TATCAACGCAGAGTACTTTTTTTTT53590.2679777356956445No Hit
GTCCTACAGTGGACATTTCTAAATT32410.1620667739110998No Hit
CTGTAGGACGTGGAATATGGCAAGA31030.15516605968717762No Hit
GTCCTAAAGTGTGTATTTCTCATTT28790.143964900367188No Hit
CTTTAGGACGTGAAATATGGCGAGG27700.1385143362338002No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27620.13811429482951484No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22480.11241163460418153No Hit
GTCCTACAGTGTGCATTTCTCATTT20700.10351071335883263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC551.1353244E-515.53575715
GGTATCA13150.014.460991
TAGGACC6700.011.7716184
AAGGCGT1657.2759576E-1211.5212196
GTATACG600.00583948911.0927841
GTATCAA30650.010.7644721
CGCCCTA1951.8189894E-1210.71153116
GCACCGT803.753373E-410.6931326
TGGTATC4100.010.6609512
GTACCGT1553.6325218E-910.4248456
GTCGAGG750.002660776710.12751919
CTGTAGG19100.010.1054681
CTTGGAC2450.010.0839163
TGTAGGA19100.010.04939652
GTAGGAC18450.010.0429253
ACCGTAT951.6356376E-410.005278
TCGAAAC951.6543255E-49.99401216
GGCGAGG7800.09.98144819
GTGCGCC1054.1080042E-59.95189611
GTGGTAT4400.09.9402871
Produced by FastQC (version 0.11.5)