FastQCFastQC Report
Thu 26 May 2016
SRR1512275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610839
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58560.3635372622589843No Hit
GGTATCAACGCAGAGTACTTTTTTT39190.24328936659715836No Hit
TATCAACGCAGAGTACTTTTTTTTT34540.21442242210425747No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16860.10466595358071167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.014.66546251
CGGTTTG550.003049662312.10086314
TCTTATA1302.6120688E-911.69317152
TAAGCGA1604.5474735E-1111.282083513
CGCCCTA2050.011.13249816
ACCGCTT600.005946604511.065518
TAGGACG700.001490139910.8582844
ACCGTGC1053.533305E-610.8396848
GGACCGT803.7671524E-410.6886236
GCGACTA1553.603418E-910.42923516
TGGTATC3200.010.3913932
GTGGTAT3850.010.3627851
GTAGGAC1201.5212845E-610.2927483
CTTATAC1201.5212845E-610.2927483
TCTATAC750.002645342410.1343983
GTCATAA951.6452573E-49.999181
GTACTAA1054.1081297E-59.9515651
GTGTAGA2201.8189894E-129.9310041
GTCTTAC1151.0272048E-59.91223051
TCGAAAC1252.5382487E-69.88936916
Produced by FastQC (version 0.11.5)