FastQCFastQC Report
Thu 26 May 2016
SRR1512275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610839
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59720.3707384785195789No Hit
GGTATCAACGCAGAGTACTTTTTTT41330.25657436900894504No Hit
TATCAACGCAGAGTACTTTTTTTTT40450.2511113773629767No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18220.11310875885175366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.014.8834181
ACCGTCC604.0757138E-412.6727188
TAATACT1751.8189894E-1211.4040284
TTTAGAC700.00148706410.8609783
CTAACCC1252.2286986E-710.6437593
CCGTACA1252.2613676E-710.632524517
TGCACCG909.4806266E-510.5592845
TAGACAG2100.010.4084385
TAAGACT1606.1336323E-910.0973164
ATTACTC856.571837E-410.0623783
TCGAAAC856.6359545E-410.05175716
CTCCTAA1054.0427138E-59.9661181
CTAATAC1151.0219124E-59.9165463
CATGACT1554.039066E-89.80991654
CGCCCTA2054.1836756E-119.7248716
CTTACAC2251.8189894E-129.7145413
GTATCAA25500.09.6250311
TAAGGGT1201.6928632E-59.5033574
TAGGACT1002.7452185E-49.5033554
ACCGGGG1606.696973E-89.49391615
Produced by FastQC (version 0.11.5)