FastQCFastQC Report
Thu 26 May 2016
SRR1512274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543279
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61780.40031646902471946No Hit
GTCCTACAGTGGACATTTCTAAATT40500.2624282453140359No Hit
GGTATCAACGCAGAGTACTTTTTTT37780.24480343476455005No Hit
CTGTAGGACGTGGAATATGGCAAGA37200.2410452031032626No Hit
GTCCTAAAGTGTGTATTTCTCATTT37090.24033243502957014No Hit
TATCAACGCAGAGTACTTTTTTTTT35560.23041847909548435No Hit
CTTTAGGACGTGAAATATGGCGAGG33540.21732946537858674No Hit
GTCCTACAGTGTGCATTTCTCATTT20280.13140851394984318No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17920.11611639891425983No Hit
CTGAAGGACCTGGAATATGGCGAGA17910.11605160181665142No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16120.10445292134474712No Hit
GTCCTTCAGTGTGCATTTCTCATTT15820.10250900841649502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1900.015.50257618
GGTATCA10300.013.9272321
CCAACGA1001.0024451E-813.30437819
CCAACGT1207.366907E-1012.670835519
TAGGACC6050.012.2491724
CACCTTT5350.011.90654214
AAGTCGT1406.7848305E-1011.5372567
AGTCGTC1406.9667294E-1011.5188748
CCACCTT5800.011.4659313
GTATAGA1701.0913936E-1111.176511
CCGGTTT2050.011.11692212
TCTCGGA600.005861227411.08698119
GGCGAGG9200.010.94925519
CGGTTTC2000.010.92539313
TAGGACA6700.010.9190144
GTATCAA24500.010.8959561
AAGACCG700.001490127410.8582415
TGTAGGA22450.010.8347632
CAAGTCG1355.6763383E-810.55662256
ACCTTTT5700.010.50824815
Produced by FastQC (version 0.11.5)