FastQCFastQC Report
Thu 26 May 2016
SRR1512274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543279
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71270.4618089146550947No Hit
GGTATCAACGCAGAGTACTTTTTTT47270.3062958803949254No Hit
TATCAACGCAGAGTACTTTTTTTTT45180.29275328699476894No Hit
GTCCTACAGTGGACATTTCTAAATT41290.2675472160250998No Hit
CTGTAGGACGTGGAATATGGCAAGA40260.26087311497143423No Hit
GTCCTAAAGTGTGTATTTCTCATTT38430.2490152461090963No Hit
CTTTAGGACGTGAAATATGGCGAGG35230.22828017487440702No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21760.14099848439588694No Hit
CTGAAGGACCTGGAATATGGCGAGA19980.12946460102159105No Hit
GTCCTACAGTGTGCATTTCTCATTT19950.12927020972876582No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17910.11605160181665142No Hit
CTGTAGGACCTGGAATATGGCGAGA15990.10361055907583787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.013.6063421
TAGGACC6750.013.2335144
GGCGAGG9400.011.91782419
TCCAACG1950.011.68475118
CCAACGA1157.136077E-811.55774119
TGGTATC4300.011.4913722
GTATTAC1751.8189894E-1211.41223051
ATTTAGA6550.011.0347271
ATTTCCG803.7875588E-410.682322514
CCCGTTT1252.2412678E-710.63932812
AAATGTC6000.010.6135137
TGTAGGA22750.010.5675952
GATATAC5050.010.5459561
CCGTTTC1451.4446414E-810.48175713
ACTGTTC5400.010.3846988
TAGAAAT6550.010.3007344
TGTCCCG1201.5244295E-610.29101811
CGCCCTA1201.5371606E-610.284348516
GAACAGT4900.010.2805286
GTGGTAT5300.010.2279421
Produced by FastQC (version 0.11.5)