FastQCFastQC Report
Thu 26 May 2016
SRR1512273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359770
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70120.29714760336812485No Hit
GTCCTACAGTGGACATTTCTAAATT48310.20472334168160455No Hit
CTGTAGGACGTGGAATATGGCAAGA44830.18997614174262745No Hit
GTCCTAAAGTGTGTATTTCTCATTT43730.1853146704975485No Hit
GGTATCAACGCAGAGTACTTTTTTT43130.18277204981841452No Hit
TATCAACGCAGAGTACTTTTTTTTT40900.17332197629429988No Hit
CTTTAGGACGTGAAATATGGCGAGG36660.15535412349508637No Hit
GTCCTACAGTGTGCATTTCTCATTT23690.10039113981447345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.013.2944461
ATTAGAC957.386552E-813.00087453
TAGGACC8200.012.6283614
GTCTTAC2200.011.2275491
GTGGTAT5550.011.12641
GGCGAGG10950.011.11263419
CGTGCGC1053.507961E-610.84678410
ACCGTCC909.7030905E-510.535628
TGTAGGA27900.010.2834082
TCTATAC2151.8189894E-1210.1634743
TCAATAC1502.4436304E-810.1340153
GTCCTAC23250.010.0927221
GTCCTAA12900.010.0893961
ATAGGAC2550.010.05953
TAGAACG951.6442251E-410.0002474
GTAGGAC27000.09.9932643
TTTAGAA8300.09.95807652
ACTATCG4100.09.95623911
TCCTACA25750.09.9245172
ATAGTAC1356.4398955E-79.8525143
Produced by FastQC (version 0.11.5)