FastQCFastQC Report
Thu 26 May 2016
SRR1512273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359770
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82180.3482542790187179No Hit
GGTATCAACGCAGAGTACTTTTTTT52750.22353873470719604No Hit
GTCCTACAGTGGACATTTCTAAATT49880.21137653245867183No Hit
TATCAACGCAGAGTACTTTTTTTTT49540.20993571407382922No Hit
CTGTAGGACGTGGAATATGGCAAGA47750.2023502290477462No Hit
GTCCTAAAGTGTGTATTTCTCATTT47200.2000194934252067No Hit
CTTTAGGACGTGAAATATGGCGAGG39510.1674315717209728No Hit
GTCCTACAGTGTGCATTTCTCATTT25290.1071714616254974No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24610.10428982485581222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGT250.006016939419.008766
TAACCCG402.755299E-416.6301985
GGTATCA17600.013.99517251
ACCGACC655.4291148E-513.160478
TAGGACC7750.012.383994
TAGACAG3500.012.2181035
GCGTGCG1251.4024408E-912.165359
GTCTACG550.003044607112.1039321
AATAGCG550.00306207812.0946895
GGCGTGC1603.6379788E-1211.88098058
ACGAAAT1701.8189894E-1211.73473212
TAGGACT3550.011.2424744
GTCCTAA14350.011.0013851
CGTGCAA700.001492231910.85685210
GTAGGAC25800.010.7173293
TGTAGGA27750.010.614582
CGCCCTA2450.010.46267316
CTGTAGG27750.010.3498751
CGAAATC1752.3646862E-1010.31291613
ACCGTGC1201.514667E-610.2968498
Produced by FastQC (version 0.11.5)