FastQCFastQC Report
Thu 26 May 2016
SRR1512272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905164
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65990.34637438036830426No Hit
GTCCTACAGTGGACATTTCTAAATT54140.2841750106552507No Hit
CTGTAGGACGTGGAATATGGCAAGA51770.27173513671264No Hit
GTCCTAAAGTGTGTATTTCTCATTT48500.2545712600070125No Hit
CTTTAGGACGTGAAATATGGCGAGG40390.21200274622027288No Hit
GGTATCAACGCAGAGTACTTTTTTT39270.20612398722629652No Hit
TATCAACGCAGAGTACTTTTTTTTT36820.19326420192697322No Hit
GTCCTACAGTGTGCATTTCTCATTT28390.1490160427133832No Hit
CTGAAGGACCTGGAATATGGCGAGA22120.11610549013103333No Hit
CTGTAGGACCTGGAATATGGCGAGA21130.11090908709171494No Hit
GTCCTTCAGTGTGCATTTCTCATTT19900.10445294998225874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.013.5714484
GGTATCA11950.013.4348111
CGCCCTA1354.660251E-911.26997716
TCGAACT855.2940177E-511.18268119
TTGCGAG600.005866029311.0859818
GTATTAG2150.011.0462361
GTCCCGC1658.0035534E-1110.9353712
GTACTAG1053.467956E-610.8568721
CTAGGAC3250.010.815973
GGCGAGG11550.010.7808819
GTCCTAC24450.010.7625131
ATAGGAC2300.010.7397123
TCCTATA2300.010.7397122
TGTAGGA30050.010.6544882
GTCCTAA14300.010.6291061
AGGACCT17500.010.585735
AAATGTC6700.010.4925537
CCGCCCT1451.4235411E-810.49218415
GTCCTAT2450.010.4691271
GTGGTAT3600.010.29140951
Produced by FastQC (version 0.11.5)